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auxiliary_sets.@id
/auxiliary-sets/IGVFDS9190KTAF/
6 items
in vitro CRISPR screen using flow cytometry
seqspec, reads
yaml, fastq
CRISPR FlowFISH screen
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin E for 80-90% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
mixed CRISPR FlowFISH screen sorted on the expression of FAM3C integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
in vitro CRISPR screen using flow cytometry
reads, seqspec
fastq, yaml
CRISPR FlowFISH screen
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin B for 10-20% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
mixed CRISPR FlowFISH screen sorted on the expression of FAM3C integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
in vitro CRISPR screen using flow cytometry
seqspec, reads
yaml, fastq
CRISPR FlowFISH screen
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin D for 55-65% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
mixed CRISPR FlowFISH screen sorted on the expression of FAM3C integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
in vitro CRISPR screen using flow cytometry
seqspec, reads
yaml, fastq
CRISPR FlowFISH screen
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin C for 35-45% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
mixed CRISPR FlowFISH screen sorted on the expression of FAM3C integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
in vitro CRISPR screen using flow cytometry
seqspec, reads
yaml, fastq
CRISPR FlowFISH screen
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin F for 80-100% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
mixed CRISPR FlowFISH screen sorted on the expression of FAM3C integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
in vitro CRISPR screen using flow cytometry
seqspec, reads
yaml, fastq
CRISPR FlowFISH screen
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin A for 0-10% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
mixed CRISPR FlowFISH screen sorted on the expression of FAM3C integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci