IGVF

/

MeasurementSet

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Assay

in vitro CRISPR screen using single-cell RNA-seq
1

Preferred Assay Title

Perturb-seq
1

Auxiliary Data

gRNA sequencing
1

Construct Library Data

guide library
1

Investigated Gene

BACH2
1
BATF
1
BATF3
1
BHLHE40
1
CREM
1
DNMT1
1
EOMES
1
FLI1
1
FOXD2
1
FOXO1
1

Sequencing Platform

Illumina NovaSeq 6000
1

auxiliary_sets.@id

/auxiliary-sets/IGVFDS9108NLTS/

1 item

/measurement-sets/IGVFDS6764PIJI/

IGVFDS6764PIJI

charles-gersbach:cd8-tcells-perturb-seq-crispra-gex-rep1

/assay-terms/OBI_0003660/

/awards/HG012053/

/human-donors/IGVFDO7605NZFO/

experimental data

/labs/charles-gersbach/

Perturb-seq

https://www.protocols.io/view/perturb-seq-characterizing-regulators-of-t-cell-fu-c5s9y6h6, https://www.protocols.io/view/crispra-tiling-screens-c5rfy53n

/primary-cells/IGVFSM8036VPRU/

released

CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes

Homo sapiens

c574903e-13e8-48c1-bf6d-1f387dafd55f