in vitro CRISPR screen using single-cell RNA-seq

reads, seqspec

fastq, yaml

TAP-seq

Homo sapiens (male) HCT116 cell line modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

mixed CRISPR TAP-seq integrating a guide (sgRNA) library targeting accessible genome regions in 29 genomic loci