IGVF

/

MeasurementSet

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Assay

in vitro CRISPR screen using single-cell RNA-seq
1

Preferred Assay Title

Perturb-seq
1

Auxiliary Data

gRNA sequencing
1

Construct Library Data

guide library
1

Investigated Gene

BACH2
1
BATF
1
BATF3
1
BHLHE40
1
CREM
1
DNMT1
1
EOMES
1
FLI1
1
FOXD2
1
FOXO1
1

Sequencing Platform

Illumina NovaSeq 6000
1

auxiliary_sets.@id

/auxiliary-sets/IGVFDS8029DSPH/

1 item

/measurement-sets/IGVFDS9813VHFD/

IGVFDS9813VHFD

charles-gersbach:cd8-tcells-perturb-seq-crispri-gex-rep1

/assay-terms/OBI_0003660/

/awards/HG012053/

/human-donors/IGVFDO7605NZFO/

experimental data

/labs/charles-gersbach/

Perturb-seq

https://www.protocols.io/view/perturb-seq-characterizing-regulators-of-t-cell-fu-c5s9y6h6, https://www.protocols.io/view/crispri-tiling-screens-c5rby52n

/primary-cells/IGVFSM3795JDHP/

released

CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes

Homo sapiens

3d7040d7-c833-412d-af01-35875b358070