audit_missing_seqspec

audit_no_files

audit_missing_auxiliary_set

audit_biosample_age

Charles Gersbach

High-Throughput Functional Annotation of Gene Regulatory Elements and Variants Critical to Complex Cellular Phenotypes

Charles Gersbach, Duke

CRISPR FACS screen

guide (sgRNA) library targeting transcription start sites in many genes

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes

Accession

Aliases

Alternate Accessions

Assay Term

Award

Donors

Taxa

File Set Type

Functional Assay Mechanisms

Preferred Assay Title

Protocols

Samples

Sequencing Library Types

Status

Summary

Assay Targeted Genes

UUID

MeasurementSet

Measurement Set Details

Sample

Files

Provenance

Quality

Object Type

Assay

Auxiliary Data

Construct Library Data

Readout Genes

Classification

Modification

Audit category: WARNING

Audit Category: Internal Action

Externally Hosted

auxiliary_sets.@id

Report