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in vitro CRISPR screen using flow cytometry
seqspec, reads
yaml, fastq
CRISPR FlowFISH screen
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin C for 35-45% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
mixed CRISPR FlowFISH screen sorted on the expression of MYC integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci