in vitro CRISPR screen using single-cell RNA-seq

reads, seqspec

fastq, yaml

TAP-seq

Homo sapiens (male) HCT116 cell line treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

mixed CRISPR TAP-seq integrating a guide (sgRNA) library targeting accessible genome regions in 29 genomic loci