in vitro CRISPR screen using single-cell RNA-seq

reads, seqspec

fastq, yaml

Perturb-seq

Homo sapiens astrocyte cell treated with 10 ng/mL Neurotrophin-3, 20 ng/mL Brain-Derived Neurotrophic Factor, modified with CRISPRa Sp-dCas9-2xVP64-2A-Puro, transduced (lentivirus) with a guide library

CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in many genes