in vitro CRISPR screen using single-cell RNA-seq

reads, seqspec

fastq, yaml

Perturb-seq

Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes