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in vitro CRISPR screen using flow cytometry
seqspec, reads
yaml, fastq
CRISPR FlowFISH screen
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin E for 80-90% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
mixed CRISPR FlowFISH screen sorted on the expression of FAM3C integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci