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INDEXED
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MeasurementSet
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IGVFDS9813VHFD
IGVFDS9813VHFD
released
Json view
File Set Type
experimental data
Summary
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
Assay Term
in vitro CRISPR screen using single-cell RNA-seq
Sample Summaries
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
Construct Library Sets
IGVFDS0821YMMV
guide (sgRNA) library targeting transcription start sites in 24 genes
Protocols
https://www.protocols.io/view/perturb-seq-characterizing-regulators-of-t-cell-fu-c5s9y6h6
https://www.protocols.io/view/crispri-tiling-screens-c5rby52n
Aliases
charles-gersbach:cd8-tcells-perturb-seq-crispri-gex-rep1
Publications
Transcriptional and epigenetic regulators of human CD8+ T cell function identified through orthogonal CRISPR screens
Sequencing Results (Illumina)
8 items
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Associated seqspec Files
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI1399GORD
fastq
reads
R1
1
IGVFFI2302TGOE
Illumina NovaSeq 6000
3
7.1 GB
Charles Gersbach, Duke
validated
IGVFFI1822KONB
fastq
reads
R2
1
IGVFFI7623PTAA
Illumina NovaSeq 6000
1
18 GB
Charles Gersbach, Duke
validated
IGVFFI2739KXTR
fastq
reads
R1
1
IGVFFI7623PTAA
Illumina NovaSeq 6000
1
7.1 GB
Charles Gersbach, Duke
validated
IGVFFI6523JOQO
fastq
reads
R1
1
IGVFFI7648DJAF
Illumina NovaSeq 6000
4
7.1 GB
Charles Gersbach, Duke
validated
IGVFFI7019QDVE
fastq
reads
R1
1
IGVFFI3571CWKX
Illumina NovaSeq 6000
2
7.0 GB
Charles Gersbach, Duke
validated
IGVFFI7384WZTF
fastq
reads
R2
1
IGVFFI2302TGOE
Illumina NovaSeq 6000
3
18 GB
Charles Gersbach, Duke
validated
IGVFFI7645VURG
fastq
reads
R2
1
IGVFFI3571CWKX
Illumina NovaSeq 6000
2
17 GB
Charles Gersbach, Duke
validated
IGVFFI7716LYLL
fastq
reads
R2
1
IGVFFI7648DJAF
Illumina NovaSeq 6000
4
18 GB
Charles Gersbach, Duke
validated
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM3795JDHP
PrimaryCell
CD8-positive, alpha-beta memory T cell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO7605NZFO
charles-gersbach:cd8-tcells-donor-6
unspecified
Homo sapiens
File Sets Using This Measurement Set as an Input
1 item
Accession
Type
Summary
Aliases
Lab
IGVFDS3319RFFN
AnalysisSet
interference Perturb-seq: filtered feature barcode matrix
charles-gersbach:cd8-tcells-intermediate-analysis-set-perturb-seq-crispri
Charles Gersbach, Duke
Auxiliary Datasets
1 item
Accession
Type
Summary
Files
Aliases
Lab
IGVFDS8029DSPH
AuxiliarySet
gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
IGVFFI1875HEKG
,
IGVFFI9522NHQX
,
IGVFFI5512GZXV
,
IGVFFI4222KPGA
,
IGVFFI5492CFOS
,
IGVFFI7250XPAW
,
IGVFFI5465GDKG
,
IGVFFI3480BTJU
charles-gersbach:cd8-tcells-perturb-seq-crispri-grna-rep1
Charles Gersbach, Duke
Attribution
Award
HG012053
Principal Investigator(s)
Charles Gersbach
,
Gregory Crawford
,
Tim Reddy
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke