IGVFDS9717YXWE

released

File Set Type

experimental data

Summary

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

MYC

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Construct Library Sets

IGVFDS7857SYCK guide (sgRNA) library targeting accessible genome regions in many genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinA-PCR1_Rep1_S135

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinA-PCR1_Rep2_S190

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinA-PCR2_Rep1_S141

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinA-PCR2_Rep2_S196

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinA-PCR3_Rep1_S147

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinA-PCR3_Rep2_S202

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinA-PCR4_Rep1_S153

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinA-PCR4_Rep2_S208

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM9689GGGO

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS3773NYHJ

AuxiliarySet

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFFI0625IJAQ, IGVFFI9925PDQT, IGVFFI5272TWLQ, IGVFFI1923OXRY, IGVFFI2912HULO, IGVFFI7847XCEY, IGVFFI0779HVAM, IGVFFI7772BVHA

jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinA-PCR1_Rep1_S135
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinA-PCR1_Rep2_S190
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinA-PCR2_Rep1_S141
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinA-PCR2_Rep2_S196
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinA-PCR3_Rep1_S147
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinA-PCR3_Rep2_S202
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinA-PCR4_Rep1_S153
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinA-PCR4_Rep2_S208

Jesse Engreitz, Stanford

IGVFDS5394TNHK

AuxiliarySet

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

IGVFFI9629PGSJ