IGVFDS9546RHPY

released
File Set Type
experimental data
Summary
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Assay Term
Targeted Genes
Sample Summaries
  • HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
Construct Library Sets
  • IGVFDS5413RKIA guide (sgRNA) library targeting accessible genome regions in many genomic loci
Protocols
Aliases
jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinB-PCR1-Rep1_S14
jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinB-PCR2-Rep1_S20
jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinB-PCR3-Rep1_S68
jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinB-PCR4-Rep1_S74

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Auxiliary Datasets

2 items
Files
AuxiliarySet
cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets
  • jesse-engreitz:auxiliary_sortparams_CRUDO_SSFA2-Auxin0hrs-FF2
Jesse Engreitz, Stanford
AuxiliarySet
gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin0hrs-FF2-BinB-PCR1-Rep1_S14
  • jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin0hrs-FF2-BinB-PCR2-Rep1_S20
  • jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin0hrs-FF2-BinB-PCR3-Rep1_S68
  • jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin0hrs-FF2-BinB-PCR4-Rep1_S74
Jesse Engreitz, Stanford

Documents

1 item
Page
Description
Type
Download
Preview
overview of experimental design
experimental protocol

Attribution