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IGVFDS9498BKLC

released
File Set Type
experimental data
Summary
mixed CRISPR FlowFISH screen sorted on the expression of KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Assay Term
in vitro CRISPR screen using flow cytometry
Targeted Genes
Sample Summaries
  • Homo sapiens (male) HCT116 cell line (sorting details: FACS bin E for 80-90% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
Protocols
Aliases
jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinE-PCR1_S29
jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinE-PCR2_S35
jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinE-PCR3_S125
jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinE-PCR4_S131
Attribution
Jesse Engreitz, Stanford

Sequencing Results

4 items
Read Names
fastq
reads
1
TCGCCTTG:CTCT
8.1 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TTACGCAC:CTCT
12 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TCGCCTTG:ATTG
16 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TTACGCAC:ATTG
20 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Associated Construct Library Sets
Summary
InVitroSystem
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin E for 80-90% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Associated Construct Library Sets

1 item
Accession
Summary
Library Design Files
guide (sgRNA) library targeting accessible genome regions in many genomic loci

Auxiliary Datasets

1 item
Accession
Type
Summary
Files
Aliases
Lab
AuxiliarySet
cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets
  • jesse-engreitz:auxiliary_sortparams_CRUDO_KITLG-Auxin0hrs-FF3
Jesse Engreitz, Stanford

Documents

1 item
Page
Description
Type
Download
Preview
overview of experimental design
experimental protocol