IGVFDS9381QGYL

released
{
    "@context": "/terms/",
    "@id": "/measurement-sets/IGVFDS9381QGYL/",
    "@type": [
        "MeasurementSet",
        "FileSet",
        "Item"
    ],
    "accession": "IGVFDS9381QGYL",
    "aliases": [
        "jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-BioRep1-FFrep1-BinC_AmpliconSequencing"
    ],
    "assay_term": {
        "@id": "/assay-terms/OBI_0003661/",
        "status": "released",
        "term_name": "in vitro CRISPR screen using flow cytometry"
    },
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "missing sequencing kit",
                "detail": "Measurement set [IGVFDS9381QGYL](/measurement-sets/IGVFDS9381QGYL/) has sequence file(s) [IGVFFI6632MKGP](/sequence-files/IGVFFI6632MKGP/), [IGVFFI7110RRRE](/sequence-files/IGVFFI7110RRRE/), [IGVFFI6966HDZC](/sequence-files/IGVFFI6966HDZC/), [IGVFFI9484UUOC](/sequence-files/IGVFFI9484UUOC/), [IGVFFI9524QHTO](/sequence-files/IGVFFI9524QHTO/), [IGVFFI6494YIJY](/sequence-files/IGVFFI6494YIJY/), [IGVFFI3640WYRU](/sequence-files/IGVFFI3640WYRU/), [IGVFFI8260HFWJ](/sequence-files/IGVFFI8260HFWJ/), [IGVFFI1366FCMB](/sequence-files/IGVFFI1366FCMB/), [IGVFFI5420TYCM](/sequence-files/IGVFFI5420TYCM/), [IGVFFI9725WUTH](/sequence-files/IGVFFI9725WUTH/), [IGVFFI2781TBIG](/sequence-files/IGVFFI2781TBIG/), [IGVFFI6983PXUZ](/sequence-files/IGVFFI6983PXUZ/), [IGVFFI0206BXVL](/sequence-files/IGVFFI0206BXVL/), [IGVFFI3338KEAW](/sequence-files/IGVFFI3338KEAW/), [IGVFFI1585QWET](/sequence-files/IGVFFI1585QWET/), [IGVFFI4270YUDC](/sequence-files/IGVFFI4270YUDC/), [IGVFFI6047UCAM](/sequence-files/IGVFFI6047UCAM/), [IGVFFI3535BCRZ](/sequence-files/IGVFFI3535BCRZ/), [IGVFFI3620RRIV](/sequence-files/IGVFFI3620RRIV/), [IGVFFI0614TWOQ](/sequence-files/IGVFFI0614TWOQ/), [IGVFFI8362EEHI](/sequence-files/IGVFFI8362EEHI/), [IGVFFI1966ORWS](/sequence-files/IGVFFI1966ORWS/), [IGVFFI1687AQIM](/sequence-files/IGVFFI1687AQIM/), [IGVFFI3879ALXZ](/sequence-files/IGVFFI3879ALXZ/), [IGVFFI6748CQCZ](/sequence-files/IGVFFI6748CQCZ/), [IGVFFI8012RSML](/sequence-files/IGVFFI8012RSML/), [IGVFFI9808OVLJ](/sequence-files/IGVFFI9808OVLJ/), [IGVFFI5167VAFJ](/sequence-files/IGVFFI5167VAFJ/), [IGVFFI9402KQMY](/sequence-files/IGVFFI9402KQMY/), [IGVFFI4699UFBK](/sequence-files/IGVFFI4699UFBK/), [IGVFFI2924HYNO](/sequence-files/IGVFFI2924HYNO/), [IGVFFI8714LANI](/sequence-files/IGVFFI8714LANI/), [IGVFFI5356GWER](/sequence-files/IGVFFI5356GWER/), [IGVFFI5478QGDN](/sequence-files/IGVFFI5478QGDN/), [IGVFFI0634IPML](/sequence-files/IGVFFI0634IPML/), [IGVFFI8624OQLF](/sequence-files/IGVFFI8624OQLF/), [IGVFFI7389SJVG](/sequence-files/IGVFFI7389SJVG/), [IGVFFI1960ESWO](/sequence-files/IGVFFI1960ESWO/), [IGVFFI9497BBLJ](/sequence-files/IGVFFI9497BBLJ/), [IGVFFI4608HSLS](/sequence-files/IGVFFI4608HSLS/), [IGVFFI4852GHJP](/sequence-files/IGVFFI4852GHJP/), [IGVFFI4528QCPU](/sequence-files/IGVFFI4528QCPU/), [IGVFFI7577TTSA](/sequence-files/IGVFFI7577TTSA/), [IGVFFI0346PFTX](/sequence-files/IGVFFI0346PFTX/), [IGVFFI4969BYGH](/sequence-files/IGVFFI4969BYGH/), [IGVFFI4287YWHI](/sequence-files/IGVFFI4287YWHI/), [IGVFFI8386TEDH](/sequence-files/IGVFFI8386TEDH/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/measurement-sets/IGVFDS9381QGYL/"
            },
            {
                "category": "missing sequence specification",
                "detail": "Measurement set [IGVFDS9381QGYL](/measurement-sets/IGVFDS9381QGYL/) has sequence file(s): [IGVFFI6632MKGP](/sequence-files/IGVFFI6632MKGP/), [IGVFFI7110RRRE](/sequence-files/IGVFFI7110RRRE/), [IGVFFI6966HDZC](/sequence-files/IGVFFI6966HDZC/), [IGVFFI9484UUOC](/sequence-files/IGVFFI9484UUOC/), [IGVFFI9524QHTO](/sequence-files/IGVFFI9524QHTO/), [IGVFFI6494YIJY](/sequence-files/IGVFFI6494YIJY/), [IGVFFI3640WYRU](/sequence-files/IGVFFI3640WYRU/), [IGVFFI8260HFWJ](/sequence-files/IGVFFI8260HFWJ/), [IGVFFI1366FCMB](/sequence-files/IGVFFI1366FCMB/), [IGVFFI5420TYCM](/sequence-files/IGVFFI5420TYCM/), [IGVFFI9725WUTH](/sequence-files/IGVFFI9725WUTH/), [IGVFFI2781TBIG](/sequence-files/IGVFFI2781TBIG/), [IGVFFI6983PXUZ](/sequence-files/IGVFFI6983PXUZ/), [IGVFFI0206BXVL](/sequence-files/IGVFFI0206BXVL/), [IGVFFI3338KEAW](/sequence-files/IGVFFI3338KEAW/), [IGVFFI1585QWET](/sequence-files/IGVFFI1585QWET/), [IGVFFI4270YUDC](/sequence-files/IGVFFI4270YUDC/), [IGVFFI6047UCAM](/sequence-files/IGVFFI6047UCAM/), [IGVFFI3535BCRZ](/sequence-files/IGVFFI3535BCRZ/), [IGVFFI3620RRIV](/sequence-files/IGVFFI3620RRIV/), [IGVFFI0614TWOQ](/sequence-files/IGVFFI0614TWOQ/), [IGVFFI8362EEHI](/sequence-files/IGVFFI8362EEHI/), [IGVFFI1966ORWS](/sequence-files/IGVFFI1966ORWS/), [IGVFFI1687AQIM](/sequence-files/IGVFFI1687AQIM/), [IGVFFI3879ALXZ](/sequence-files/IGVFFI3879ALXZ/), [IGVFFI6748CQCZ](/sequence-files/IGVFFI6748CQCZ/), [IGVFFI8012RSML](/sequence-files/IGVFFI8012RSML/), [IGVFFI9808OVLJ](/sequence-files/IGVFFI9808OVLJ/), [IGVFFI5167VAFJ](/sequence-files/IGVFFI5167VAFJ/), [IGVFFI9402KQMY](/sequence-files/IGVFFI9402KQMY/), [IGVFFI4699UFBK](/sequence-files/IGVFFI4699UFBK/), [IGVFFI2924HYNO](/sequence-files/IGVFFI2924HYNO/), [IGVFFI8714LANI](/sequence-files/IGVFFI8714LANI/), [IGVFFI5356GWER](/sequence-files/IGVFFI5356GWER/), [IGVFFI5478QGDN](/sequence-files/IGVFFI5478QGDN/), [IGVFFI0634IPML](/sequence-files/IGVFFI0634IPML/), [IGVFFI8624OQLF](/sequence-files/IGVFFI8624OQLF/), [IGVFFI7389SJVG](/sequence-files/IGVFFI7389SJVG/), [IGVFFI1960ESWO](/sequence-files/IGVFFI1960ESWO/), [IGVFFI9497BBLJ](/sequence-files/IGVFFI9497BBLJ/), [IGVFFI4608HSLS](/sequence-files/IGVFFI4608HSLS/), [IGVFFI4852GHJP](/sequence-files/IGVFFI4852GHJP/), [IGVFFI4528QCPU](/sequence-files/IGVFFI4528QCPU/), [IGVFFI7577TTSA](/sequence-files/IGVFFI7577TTSA/), [IGVFFI0346PFTX](/sequence-files/IGVFFI0346PFTX/), [IGVFFI4969BYGH](/sequence-files/IGVFFI4969BYGH/), [IGVFFI4287YWHI](/sequence-files/IGVFFI4287YWHI/), [IGVFFI8386TEDH](/sequence-files/IGVFFI8386TEDH/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_missing_seqspec",
                "path": "/measurement-sets/IGVFDS9381QGYL/"
            },
            {
                "category": "mismatched status",
                "detail": "Released in vitro system [IGVFSM2175KOEY](/in-vitro-systems/IGVFSM2175KOEY/) has in progress subobject construct library set [IGVFDS2385DHZO](/construct-library-sets/IGVFDS2385DHZO/).",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_item_status",
                "path": "/in-vitro-systems/IGVFSM2175KOEY/"
            }
        ],
        "WARNING": [
            {
                "category": "missing auxiliary set",
                "detail": "Measurement set [IGVFDS9381QGYL](/measurement-sets/IGVFDS9381QGYL/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
                "level": 40,
                "level_name": "WARNING",
                "name": "audit_missing_auxiliary_set",
                "path": "/measurement-sets/IGVFDS9381QGYL/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization",
        "contact_pi": {
            "@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
            "title": "Jesse Engreitz"
        },
        "title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
    },
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            "@id": "/measurement-sets/IGVFDS5128CXIS/",
            "accession": "IGVFDS5128CXIS",
            "aliases": [
                "jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-THP1-PE2_Exp4_NegControl_AmpliconSequencing"
            ],
            "status": "released"
        }
    ],
    "control_for": [],
    "creation_timestamp": "2023-12-13T08:01:36.894323+00:00",
    "donors": [
        {
            "@id": "/human-donors/IGVFDO7359LVPI/",
            "accession": "IGVFDO7359LVPI",
            "aliases": [
                "igvf:donor_of_THP1"
            ],
            "sex": "male",
            "status": "released",
            "taxa": "Homo sapiens"
        }
    ],
    "externally_hosted": false,
    "file_set_type": "experimental data",
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                "jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-BioRep1-FFrep1-BinC-PCRrep3_R2"
            ],
            "content_type": "reads",
            "controlled_access": false,
            "creation_timestamp": "2023-12-13T09:26:25.611439+00:00",
            "file_format": "fastq",
            "file_size": 13995649,
            "href": "/sequence-files/IGVFFI8386TEDH/@@download/IGVFFI8386TEDH.fastq.gz",
            "s3_uri": "s3://igvf-files/2023/12/13/0334cab9-cb6c-4b4a-a5f6-ec98306189ee/IGVFFI8386TEDH.fastq.gz",
            "sequencing_platform": {
                "@id": "/platform-terms/EFO_0008566/",
                "status": "released",
                "term_name": "Illumina NextSeq 550"
            },
            "status": "released",
            "submitted_file_name": "230523pPPIFEnformerTHP1-THP1-PE-pPPIF_Enformer-Amp-pPPIF-BioRep1-FFRep1_BinC-PCRrep3_S27_R2_001.fastq.gz",
            "upload_status": "validated"
        }
    ],
    "input_for": [
        "/analysis-sets/IGVFDS4359OODY/"
    ],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "notes": "The readout 2e41e624-f6c7-4f0c-a07e-bc36cefdca39 was removed from this measurement set. This measurement set previously used Variant FlowFISH as a preferred_assay_title, but this enum is now removed. So it has been defaulted to Variant-EFFECTS.",
    "preferred_assay_title": "Variant-EFFECTS",
    "protocols": [
        "https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
    ],
    "release_timestamp": "2023-12-20T23:07:31.364076+00:00",
    "samples": [
        {
            "@id": "/in-vitro-systems/IGVFSM2175KOEY/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM2175KOEY",
            "aliases": [
                "jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-BioRep1-FFrep1-BinC"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                {
                    "@id": "/construct-library-sets/IGVFDS2385DHZO/",
                    "accession": "IGVFDS2385DHZO",
                    "file_set_type": "guide library",
                    "status": "in progress",
                    "summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
                }
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0001253/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:THP-1"
                    ],
                    "status": "released",
                    "summary": "THP-1",
                    "term_name": "THP-1"
                }
            ],
            "status": "released",
            "summary": "THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        }
    ],
    "schema_version": "23",
    "status": "released",
    "submitted_by": {
        "@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
        "title": "Michael Montgomery"
    },
    "submitted_files_timestamp": "2023-12-13T08:16:46.764673+00:00",
    "summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
    "targeted_genes": [
        {
            "@id": "/genes/ENSG00000108179/",
            "geneid": "ENSG00000108179",
            "name": "peptidylprolyl isomerase F",
            "status": "released",
            "symbol": "PPIF",
            "synonyms": [
                "rotamase F",
                "CYP3",
                "cyclophilin F",
                "mitochondrial cyclophilin",
                "peptidyl-prolyl cis-trans isomerase, mitochondrial",
                "CypD",
                "Cyp-D",
                "cyclophilin 3",
                "cyclophilin D",
                "CyP-M",
                "peptidyl-prolyl cis-trans isomerase F, mitochondrial",
                "PPIase F"
            ]
        }
    ],
    "uuid": "477191d3-b8df-4695-86e6-3899aa507cb0"
}