IGVFDS9218EUHT

{
    "@context": "/terms/",
    "@id": "/measurement-sets/IGVFDS9218EUHT/",
    "@type": [
        "MeasurementSet",
        "FileSet",
        "Item"
    ],
    "accession": "IGVFDS9218EUHT",
    "aliases": [
        "jesse-engreitz:240426S024-PPIFenhancervalidation-THP1_Amp_PPIF_enhancer-BioRep2-FFrep1-BinC_AmpliconSequencing"
    ],
    "assay_term": {
        "@id": "/assay-terms/OBI_0003661/",
        "status": "released",
        "term_name": "in vitro CRISPR screen using flow cytometry"
    },
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "missing sequencing kit",
                "detail": "Measurement set [IGVFDS9218EUHT](/measurement-sets/IGVFDS9218EUHT/) has sequence file(s) [IGVFFI7328YPZL](/sequence-files/IGVFFI7328YPZL/), [IGVFFI0438ZRGH](/sequence-files/IGVFFI0438ZRGH/), [IGVFFI3328DDDR](/sequence-files/IGVFFI3328DDDR/), [IGVFFI1125AQON](/sequence-files/IGVFFI1125AQON/), [IGVFFI6701BOGO](/sequence-files/IGVFFI6701BOGO/), [IGVFFI8309EPYT](/sequence-files/IGVFFI8309EPYT/), [IGVFFI4121QQPN](/sequence-files/IGVFFI4121QQPN/), [IGVFFI8927YIGQ](/sequence-files/IGVFFI8927YIGQ/), [IGVFFI3825NAAQ](/sequence-files/IGVFFI3825NAAQ/), [IGVFFI7407RHDK](/sequence-files/IGVFFI7407RHDK/), [IGVFFI0063KOHU](/sequence-files/IGVFFI0063KOHU/), [IGVFFI1859YNNK](/sequence-files/IGVFFI1859YNNK/), [IGVFFI3556JYDE](/sequence-files/IGVFFI3556JYDE/), [IGVFFI9668HUXL](/sequence-files/IGVFFI9668HUXL/), [IGVFFI8711JHMZ](/sequence-files/IGVFFI8711JHMZ/), [IGVFFI2449ODYV](/sequence-files/IGVFFI2449ODYV/), [IGVFFI3543UJOQ](/sequence-files/IGVFFI3543UJOQ/), [IGVFFI7456ORSB](/sequence-files/IGVFFI7456ORSB/), [IGVFFI8940FVEX](/sequence-files/IGVFFI8940FVEX/), [IGVFFI1313PESQ](/sequence-files/IGVFFI1313PESQ/), [IGVFFI2014RTZR](/sequence-files/IGVFFI2014RTZR/), [IGVFFI1342IRHD](/sequence-files/IGVFFI1342IRHD/), [IGVFFI8877OZBZ](/sequence-files/IGVFFI8877OZBZ/), [IGVFFI4317JNQU](/sequence-files/IGVFFI4317JNQU/), [IGVFFI8097YPEG](/sequence-files/IGVFFI8097YPEG/), [IGVFFI0216MMNK](/sequence-files/IGVFFI0216MMNK/), [IGVFFI5513BRNR](/sequence-files/IGVFFI5513BRNR/), [IGVFFI4865PLTX](/sequence-files/IGVFFI4865PLTX/), [IGVFFI3179QQMW](/sequence-files/IGVFFI3179QQMW/), [IGVFFI6935NOCA](/sequence-files/IGVFFI6935NOCA/), [IGVFFI0813NKSM](/sequence-files/IGVFFI0813NKSM/), [IGVFFI4935YMBO](/sequence-files/IGVFFI4935YMBO/), [IGVFFI1425QLSN](/sequence-files/IGVFFI1425QLSN/), [IGVFFI6719CXZL](/sequence-files/IGVFFI6719CXZL/), [IGVFFI7991TUCB](/sequence-files/IGVFFI7991TUCB/), [IGVFFI4639LCKN](/sequence-files/IGVFFI4639LCKN/), [IGVFFI1066CTSU](/sequence-files/IGVFFI1066CTSU/), [IGVFFI5074YOVW](/sequence-files/IGVFFI5074YOVW/), [IGVFFI1222AUAS](/sequence-files/IGVFFI1222AUAS/), [IGVFFI0305ZNME](/sequence-files/IGVFFI0305ZNME/), [IGVFFI4319SJHS](/sequence-files/IGVFFI4319SJHS/), [IGVFFI6727XATW](/sequence-files/IGVFFI6727XATW/), [IGVFFI5974XZKD](/sequence-files/IGVFFI5974XZKD/), [IGVFFI5739ZVWW](/sequence-files/IGVFFI5739ZVWW/), [IGVFFI1978HGBT](/sequence-files/IGVFFI1978HGBT/), [IGVFFI6048PIVE](/sequence-files/IGVFFI6048PIVE/), [IGVFFI8973PNPZ](/sequence-files/IGVFFI8973PNPZ/), [IGVFFI2588NWZQ](/sequence-files/IGVFFI2588NWZQ/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/measurement-sets/IGVFDS9218EUHT/"
            },
            {
                "category": "missing sequence specification",
                "detail": "Measurement set [IGVFDS9218EUHT](/measurement-sets/IGVFDS9218EUHT/) has sequence file(s): [IGVFFI7328YPZL](/sequence-files/IGVFFI7328YPZL/), [IGVFFI0438ZRGH](/sequence-files/IGVFFI0438ZRGH/), [IGVFFI3328DDDR](/sequence-files/IGVFFI3328DDDR/), [IGVFFI1125AQON](/sequence-files/IGVFFI1125AQON/), [IGVFFI6701BOGO](/sequence-files/IGVFFI6701BOGO/), [IGVFFI8309EPYT](/sequence-files/IGVFFI8309EPYT/), [IGVFFI4121QQPN](/sequence-files/IGVFFI4121QQPN/), [IGVFFI8927YIGQ](/sequence-files/IGVFFI8927YIGQ/), [IGVFFI3825NAAQ](/sequence-files/IGVFFI3825NAAQ/), [IGVFFI7407RHDK](/sequence-files/IGVFFI7407RHDK/), [IGVFFI0063KOHU](/sequence-files/IGVFFI0063KOHU/), [IGVFFI1859YNNK](/sequence-files/IGVFFI1859YNNK/), [IGVFFI3556JYDE](/sequence-files/IGVFFI3556JYDE/), [IGVFFI9668HUXL](/sequence-files/IGVFFI9668HUXL/), [IGVFFI8711JHMZ](/sequence-files/IGVFFI8711JHMZ/), [IGVFFI2449ODYV](/sequence-files/IGVFFI2449ODYV/), [IGVFFI3543UJOQ](/sequence-files/IGVFFI3543UJOQ/), [IGVFFI7456ORSB](/sequence-files/IGVFFI7456ORSB/), [IGVFFI8940FVEX](/sequence-files/IGVFFI8940FVEX/), [IGVFFI1313PESQ](/sequence-files/IGVFFI1313PESQ/), [IGVFFI2014RTZR](/sequence-files/IGVFFI2014RTZR/), [IGVFFI1342IRHD](/sequence-files/IGVFFI1342IRHD/), [IGVFFI8877OZBZ](/sequence-files/IGVFFI8877OZBZ/), [IGVFFI4317JNQU](/sequence-files/IGVFFI4317JNQU/), [IGVFFI8097YPEG](/sequence-files/IGVFFI8097YPEG/), [IGVFFI0216MMNK](/sequence-files/IGVFFI0216MMNK/), [IGVFFI5513BRNR](/sequence-files/IGVFFI5513BRNR/), [IGVFFI4865PLTX](/sequence-files/IGVFFI4865PLTX/), [IGVFFI3179QQMW](/sequence-files/IGVFFI3179QQMW/), [IGVFFI6935NOCA](/sequence-files/IGVFFI6935NOCA/), [IGVFFI0813NKSM](/sequence-files/IGVFFI0813NKSM/), [IGVFFI4935YMBO](/sequence-files/IGVFFI4935YMBO/), [IGVFFI1425QLSN](/sequence-files/IGVFFI1425QLSN/), [IGVFFI6719CXZL](/sequence-files/IGVFFI6719CXZL/), [IGVFFI7991TUCB](/sequence-files/IGVFFI7991TUCB/), [IGVFFI4639LCKN](/sequence-files/IGVFFI4639LCKN/), [IGVFFI1066CTSU](/sequence-files/IGVFFI1066CTSU/), [IGVFFI5074YOVW](/sequence-files/IGVFFI5074YOVW/), [IGVFFI1222AUAS](/sequence-files/IGVFFI1222AUAS/), [IGVFFI0305ZNME](/sequence-files/IGVFFI0305ZNME/), [IGVFFI4319SJHS](/sequence-files/IGVFFI4319SJHS/), [IGVFFI6727XATW](/sequence-files/IGVFFI6727XATW/), [IGVFFI5974XZKD](/sequence-files/IGVFFI5974XZKD/), [IGVFFI5739ZVWW](/sequence-files/IGVFFI5739ZVWW/), [IGVFFI1978HGBT](/sequence-files/IGVFFI1978HGBT/), [IGVFFI6048PIVE](/sequence-files/IGVFFI6048PIVE/), [IGVFFI8973PNPZ](/sequence-files/IGVFFI8973PNPZ/), [IGVFFI2588NWZQ](/sequence-files/IGVFFI2588NWZQ/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_missing_seqspec",
                "path": "/measurement-sets/IGVFDS9218EUHT/"
            }
        ],
        "WARNING": [
            {
                "category": "missing auxiliary set",
                "detail": "Measurement set [IGVFDS9218EUHT](/measurement-sets/IGVFDS9218EUHT/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
                "level": 40,
                "level_name": "WARNING",
                "name": "audit_missing_auxiliary_set",
                "path": "/measurement-sets/IGVFDS9218EUHT/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization",
        "contact_pi": {
            "@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
            "title": "Jesse Engreitz"
        },
        "title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
    },
    "control_file_sets": [
        {
            "@id": "/measurement-sets/IGVFDS4103BJLT/",
            "accession": "IGVFDS4103BJLT",
            "aliases": [
                "jesse-engreitz:240426S024-PPIFenhancervalidation-THP1_Amp_PPIF_enhancer-THP1-PE2_Exp4_NegControl_AmpliconSequencing"
            ],
            "status": "preview"
        }
    ],
    "control_for": [],
    "creation_timestamp": "2024-11-25T23:53:32.660754+00:00",
    "donors": [
        {
            "@id": "/human-donors/IGVFDO7359LVPI/",
            "accession": "IGVFDO7359LVPI",
            "aliases": [
                "igvf:donor_of_THP1"
            ],
            "sex": "male",
            "status": "released",
            "taxa": "Homo sapiens"
        }
    ],
    "externally_hosted": false,
    "file_set_type": "experimental data",
    "files": [
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            "@id": "/sequence-files/IGVFFI7328YPZL/",
            "accession": "IGVFFI7328YPZL",
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                "jesse-engreitz:240426S024-PPIFenhancervalidation-THP1_Amp_PPIF_enhancer-BioRep2-FFrep1-BinC-PCRrep2_R1"
            ],
            "content_type": "reads",
            "controlled_access": false,
            "creation_timestamp": "2024-11-26T07:12:54.566554+00:00",
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                "status": "released",
                "term_name": "Illumina NovaSeq X"
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            "upload_status": "validated"
        },
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            "@id": "/sequence-files/IGVFFI0438ZRGH/",
            "accession": "IGVFFI0438ZRGH",
            "aliases": [
                "jesse-engreitz:240426S024-PPIFenhancervalidation-THP1_Amp_PPIF_enhancer-BioRep2-FFrep1-BinC-PCRrep3_R1"
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            "upload_status": "validated"
        },
        {
            "@id": "/sequence-files/IGVFFI3328DDDR/",
            "accession": "IGVFFI3328DDDR",
            "aliases": [
                "jesse-engreitz:240426S024-PPIFenhancervalidation-THP1_Amp_PPIF_enhancer-BioRep2-FFrep1-BinC-PCRrep8_R1"
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        },
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        },
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        },
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            "@id": "/sequence-files/IGVFFI4121QQPN/",
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            ],
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        },
        {
            "@id": "/sequence-files/IGVFFI8927YIGQ/",
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            "aliases": [
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            ],
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            },
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            "upload_status": "validated"
        },
        {
            "@id": "/sequence-files/IGVFFI3825NAAQ/",
            "accession": "IGVFFI3825NAAQ",
            "aliases": [
                "jesse-engreitz:240426S024-PPIFenhancervalidation-THP1_Amp_PPIF_enhancer-BioRep2-FFrep1-BinC-PCRrep11_R1"
            ],
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            },
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            "upload_status": "validated"
        },
        {
            "@id": "/sequence-files/IGVFFI7407RHDK/",
            "accession": "IGVFFI7407RHDK",
            "aliases": [
                "jesse-engreitz:240426S024-PPIFenhancervalidation-THP1_Amp_PPIF_enhancer-BioRep2-FFrep1-BinC-PCRrep1_R1"
            ],
            "content_type": "reads",
            "controlled_access": false,
            "creation_timestamp": "2024-11-26T07:11:29.306955+00:00",
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            "sequencing_platform": {
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            },
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            "upload_status": "validated"
        },
        {
            "@id": "/sequence-files/IGVFFI0063KOHU/",
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            "href": "/sequence-files/IGVFFI8973PNPZ/@@download/IGVFFI8973PNPZ.fastq.gz",
            "s3_uri": "s3://igvf-files/2024/11/26/10d3466b-e299-4c70-a6ee-141252ea173a/IGVFFI8973PNPZ.fastq.gz",
            "sequencing_platform": {
                "@id": "/platform-terms/EFO_0022840/",
                "status": "released",
                "term_name": "Illumina NovaSeq X"
            },
            "status": "preview",
            "submitted_file_name": "240426S024-Enhancer-THP1-PPIF_Enhancer_val_guides-Amp_PPIF_enhancer-BioRep2-FFrep1-BinC-PCRrep1_S631_I2_001.fastq.gz",
            "upload_status": "validated"
        },
        {
            "@id": "/sequence-files/IGVFFI2588NWZQ/",
            "accession": "IGVFFI2588NWZQ",
            "aliases": [
                "jesse-engreitz:240426S024-PPIFenhancervalidation-THP1_Amp_PPIF_enhancer-BioRep2-FFrep1-BinC-PCRrep6_I2"
            ],
            "content_type": "reads",
            "controlled_access": false,
            "creation_timestamp": "2024-11-26T17:56:12.949489+00:00",
            "file_format": "fastq",
            "file_size": 6824914,
            "href": "/sequence-files/IGVFFI2588NWZQ/@@download/IGVFFI2588NWZQ.fastq.gz",
            "s3_uri": "s3://igvf-files/2024/11/26/7eba60c3-d882-4e8f-a029-2db01b0cffd3/IGVFFI2588NWZQ.fastq.gz",
            "sequencing_platform": {
                "@id": "/platform-terms/EFO_0022840/",
                "status": "released",
                "term_name": "Illumina NovaSeq X"
            },
            "status": "preview",
            "submitted_file_name": "240426S024-Enhancer-THP1-PPIF_Enhancer_val_guides-Amp_PPIF_enhancer-BioRep2-FFrep1-BinC-PCRrep6_S636_I2_001.fastq.gz",
            "upload_status": "validated"
        }
    ],
    "input_for": [
        "/analysis-sets/IGVFDS4833ZRTZ/"
    ],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "notes": "This measurement set previously used Variant FlowFISH as a preferred_assay_title, but this enum is now removed. So it has been defaulted to Variant-EFFECTS.",
    "preferred_assay_title": "Variant-EFFECTS",
    "protocols": [
        "https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
    ],
    "samples": [
        {
            "@id": "/in-vitro-systems/IGVFSM4994IFXA/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM4994IFXA",
            "aliases": [
                "jesse-engreitz:240426S024-PPIFenhancervalidation-THP1_Amp_PPIF_enhancer-BioRep2-FFrep1-BinC"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                {
                    "@id": "/construct-library-sets/IGVFDS4955GEYO/",
                    "accession": "IGVFDS4955GEYO",
                    "file_set_type": "guide library",
                    "status": "preview",
                    "summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
                }
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0001253/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:THP-1"
                    ],
                    "status": "released",
                    "summary": "THP-1",
                    "term_name": "THP-1"
                }
            ],
            "status": "preview",
            "summary": "THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        }
    ],
    "schema_version": "23",
    "status": "preview",
    "submitted_by": {
        "@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
        "title": "Michael Montgomery"
    },
    "submitted_files_timestamp": "2024-11-26T07:11:29.306955+00:00",
    "summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
    "targeted_genes": [
        {
            "@id": "/genes/ENSG00000108179/",
            "geneid": "ENSG00000108179",
            "name": "peptidylprolyl isomerase F",
            "status": "released",
            "symbol": "PPIF",
            "synonyms": [
                "rotamase F",
                "CYP3",
                "cyclophilin F",
                "mitochondrial cyclophilin",
                "peptidyl-prolyl cis-trans isomerase, mitochondrial",
                "CypD",
                "Cyp-D",
                "cyclophilin 3",
                "cyclophilin D",
                "CyP-M",
                "peptidyl-prolyl cis-trans isomerase F, mitochondrial",
                "PPIase F"
            ]
        }
    ],
    "uuid": "50759b3a-071c-4207-874a-f2800dfc3870"
}