IGVFDS9080CILW

released
{
    "@context": "/terms/",
    "@id": "/measurement-sets/IGVFDS9080CILW/",
    "@type": [
        "MeasurementSet",
        "FileSet",
        "Item"
    ],
    "accession": "IGVFDS9080CILW",
    "aliases": [
        "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep2-FFrep2-BinD_AmpliconSequencing"
    ],
    "assay_term": {
        "@id": "/assay-terms/OBI_0003661/",
        "status": "released",
        "term_name": "in vitro CRISPR screen using flow cytometry"
    },
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "missing sequencing kit",
                "detail": "Measurement set [IGVFDS9080CILW](/measurement-sets/IGVFDS9080CILW/) has sequence file(s) [IGVFFI2734EQMX](/sequence-files/IGVFFI2734EQMX/), [IGVFFI5745TWPY](/sequence-files/IGVFFI5745TWPY/), [IGVFFI3887XUHJ](/sequence-files/IGVFFI3887XUHJ/), [IGVFFI2777SWYW](/sequence-files/IGVFFI2777SWYW/), [IGVFFI0784JLER](/sequence-files/IGVFFI0784JLER/), [IGVFFI9548OWQS](/sequence-files/IGVFFI9548OWQS/), [IGVFFI8103TUQD](/sequence-files/IGVFFI8103TUQD/), [IGVFFI6978CSMX](/sequence-files/IGVFFI6978CSMX/), [IGVFFI9970EBKS](/sequence-files/IGVFFI9970EBKS/), [IGVFFI8239KYGI](/sequence-files/IGVFFI8239KYGI/), [IGVFFI4313OJHH](/sequence-files/IGVFFI4313OJHH/), [IGVFFI5268OCZE](/sequence-files/IGVFFI5268OCZE/), [IGVFFI1914GPLU](/sequence-files/IGVFFI1914GPLU/), [IGVFFI5958TAXN](/sequence-files/IGVFFI5958TAXN/), [IGVFFI9638URWZ](/sequence-files/IGVFFI9638URWZ/), [IGVFFI1528OAKE](/sequence-files/IGVFFI1528OAKE/), [IGVFFI2209NQUW](/sequence-files/IGVFFI2209NQUW/), [IGVFFI8517DEBM](/sequence-files/IGVFFI8517DEBM/), [IGVFFI2536UMHT](/sequence-files/IGVFFI2536UMHT/), [IGVFFI6965TRNF](/sequence-files/IGVFFI6965TRNF/), [IGVFFI8102YJHH](/sequence-files/IGVFFI8102YJHH/), [IGVFFI6911LHPR](/sequence-files/IGVFFI6911LHPR/), [IGVFFI9077FLFQ](/sequence-files/IGVFFI9077FLFQ/), [IGVFFI9857GLTM](/sequence-files/IGVFFI9857GLTM/), [IGVFFI6725YAKE](/sequence-files/IGVFFI6725YAKE/), [IGVFFI7007URDN](/sequence-files/IGVFFI7007URDN/), [IGVFFI9729UPTH](/sequence-files/IGVFFI9729UPTH/), [IGVFFI5138PGXZ](/sequence-files/IGVFFI5138PGXZ/), [IGVFFI3026SPYK](/sequence-files/IGVFFI3026SPYK/), [IGVFFI9588DRGV](/sequence-files/IGVFFI9588DRGV/), [IGVFFI0965VKXK](/sequence-files/IGVFFI0965VKXK/), [IGVFFI8387JJGS](/sequence-files/IGVFFI8387JJGS/), [IGVFFI2780WSBU](/sequence-files/IGVFFI2780WSBU/), [IGVFFI2290PFOF](/sequence-files/IGVFFI2290PFOF/), [IGVFFI4489LORS](/sequence-files/IGVFFI4489LORS/), [IGVFFI8171WIRK](/sequence-files/IGVFFI8171WIRK/), [IGVFFI4105XFFR](/sequence-files/IGVFFI4105XFFR/), [IGVFFI6943NLVX](/sequence-files/IGVFFI6943NLVX/), [IGVFFI0291WWOF](/sequence-files/IGVFFI0291WWOF/), [IGVFFI1660TKRZ](/sequence-files/IGVFFI1660TKRZ/), [IGVFFI1771JIAJ](/sequence-files/IGVFFI1771JIAJ/), [IGVFFI6466OUUC](/sequence-files/IGVFFI6466OUUC/), [IGVFFI7773FRKU](/sequence-files/IGVFFI7773FRKU/), [IGVFFI0301XWXP](/sequence-files/IGVFFI0301XWXP/), [IGVFFI7855EYVT](/sequence-files/IGVFFI7855EYVT/), [IGVFFI6225EMSE](/sequence-files/IGVFFI6225EMSE/), [IGVFFI2340ACGJ](/sequence-files/IGVFFI2340ACGJ/), [IGVFFI7618CLSB](/sequence-files/IGVFFI7618CLSB/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/measurement-sets/IGVFDS9080CILW/"
            },
            {
                "category": "missing sequence specification",
                "detail": "Measurement set [IGVFDS9080CILW](/measurement-sets/IGVFDS9080CILW/) has sequence file(s): [IGVFFI2734EQMX](/sequence-files/IGVFFI2734EQMX/), [IGVFFI5745TWPY](/sequence-files/IGVFFI5745TWPY/), [IGVFFI3887XUHJ](/sequence-files/IGVFFI3887XUHJ/), [IGVFFI2777SWYW](/sequence-files/IGVFFI2777SWYW/), [IGVFFI0784JLER](/sequence-files/IGVFFI0784JLER/), [IGVFFI9548OWQS](/sequence-files/IGVFFI9548OWQS/), [IGVFFI8103TUQD](/sequence-files/IGVFFI8103TUQD/), [IGVFFI6978CSMX](/sequence-files/IGVFFI6978CSMX/), [IGVFFI9970EBKS](/sequence-files/IGVFFI9970EBKS/), [IGVFFI8239KYGI](/sequence-files/IGVFFI8239KYGI/), [IGVFFI4313OJHH](/sequence-files/IGVFFI4313OJHH/), [IGVFFI5268OCZE](/sequence-files/IGVFFI5268OCZE/), [IGVFFI1914GPLU](/sequence-files/IGVFFI1914GPLU/), [IGVFFI5958TAXN](/sequence-files/IGVFFI5958TAXN/), [IGVFFI9638URWZ](/sequence-files/IGVFFI9638URWZ/), [IGVFFI1528OAKE](/sequence-files/IGVFFI1528OAKE/), [IGVFFI2209NQUW](/sequence-files/IGVFFI2209NQUW/), [IGVFFI8517DEBM](/sequence-files/IGVFFI8517DEBM/), [IGVFFI2536UMHT](/sequence-files/IGVFFI2536UMHT/), [IGVFFI6965TRNF](/sequence-files/IGVFFI6965TRNF/), [IGVFFI8102YJHH](/sequence-files/IGVFFI8102YJHH/), [IGVFFI6911LHPR](/sequence-files/IGVFFI6911LHPR/), [IGVFFI9077FLFQ](/sequence-files/IGVFFI9077FLFQ/), [IGVFFI9857GLTM](/sequence-files/IGVFFI9857GLTM/), [IGVFFI6725YAKE](/sequence-files/IGVFFI6725YAKE/), [IGVFFI7007URDN](/sequence-files/IGVFFI7007URDN/), [IGVFFI9729UPTH](/sequence-files/IGVFFI9729UPTH/), [IGVFFI5138PGXZ](/sequence-files/IGVFFI5138PGXZ/), [IGVFFI3026SPYK](/sequence-files/IGVFFI3026SPYK/), [IGVFFI9588DRGV](/sequence-files/IGVFFI9588DRGV/), [IGVFFI0965VKXK](/sequence-files/IGVFFI0965VKXK/), [IGVFFI8387JJGS](/sequence-files/IGVFFI8387JJGS/), [IGVFFI2780WSBU](/sequence-files/IGVFFI2780WSBU/), [IGVFFI2290PFOF](/sequence-files/IGVFFI2290PFOF/), [IGVFFI4489LORS](/sequence-files/IGVFFI4489LORS/), [IGVFFI8171WIRK](/sequence-files/IGVFFI8171WIRK/), [IGVFFI4105XFFR](/sequence-files/IGVFFI4105XFFR/), [IGVFFI6943NLVX](/sequence-files/IGVFFI6943NLVX/), [IGVFFI0291WWOF](/sequence-files/IGVFFI0291WWOF/), [IGVFFI1660TKRZ](/sequence-files/IGVFFI1660TKRZ/), [IGVFFI1771JIAJ](/sequence-files/IGVFFI1771JIAJ/), [IGVFFI6466OUUC](/sequence-files/IGVFFI6466OUUC/), [IGVFFI7773FRKU](/sequence-files/IGVFFI7773FRKU/), [IGVFFI0301XWXP](/sequence-files/IGVFFI0301XWXP/), [IGVFFI7855EYVT](/sequence-files/IGVFFI7855EYVT/), [IGVFFI6225EMSE](/sequence-files/IGVFFI6225EMSE/), [IGVFFI2340ACGJ](/sequence-files/IGVFFI2340ACGJ/), [IGVFFI7618CLSB](/sequence-files/IGVFFI7618CLSB/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_missing_seqspec",
                "path": "/measurement-sets/IGVFDS9080CILW/"
            },
            {
                "category": "mismatched status",
                "detail": "Released in vitro system [IGVFSM9682UDFK](/in-vitro-systems/IGVFSM9682UDFK/) has in progress subobject construct library set [IGVFDS2385DHZO](/construct-library-sets/IGVFDS2385DHZO/).",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_item_status",
                "path": "/in-vitro-systems/IGVFSM9682UDFK/"
            }
        ],
        "WARNING": [
            {
                "category": "missing auxiliary set",
                "detail": "Measurement set [IGVFDS9080CILW](/measurement-sets/IGVFDS9080CILW/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
                "level": 40,
                "level_name": "WARNING",
                "name": "audit_missing_auxiliary_set",
                "path": "/measurement-sets/IGVFDS9080CILW/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization",
        "contact_pi": {
            "@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
            "title": "Jesse Engreitz"
        },
        "title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
    },
    "control_file_sets": [
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            "@id": "/measurement-sets/IGVFDS4869FXYY/",
            "accession": "IGVFDS4869FXYY",
            "aliases": [
                "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-Jurkat-PE2_Exp2_NegControl_AmpliconSequencing"
            ],
            "status": "released"
        }
    ],
    "control_for": [],
    "creation_timestamp": "2023-12-13T08:48:12.412120+00:00",
    "donors": [
        {
            "@id": "/human-donors/IGVFDO1080XFGV/",
            "accession": "IGVFDO1080XFGV",
            "aliases": [
                "igvf:donor_of_Jurkat"
            ],
            "sex": "male",
            "status": "released",
            "taxa": "Homo sapiens"
        }
    ],
    "externally_hosted": false,
    "file_set_type": "experimental data",
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            "@id": "/sequence-files/IGVFFI4313OJHH/",
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            "s3_uri": "s3://igvf-files/2023/12/13/0fb1ae54-5d3c-4911-80d4-d8e2227525bf/IGVFFI7618CLSB.fastq.gz",
            "sequencing_platform": {
                "@id": "/platform-terms/EFO_0008566/",
                "status": "released",
                "term_name": "Illumina NextSeq 550"
            },
            "status": "released",
            "submitted_file_name": "230423pPPIFEnformerJurkat-Jurkat-PE-pPPIF_Enformer-Amp-pPPIF-BioRep2-FFRep2_BinD-PCRrep10_S286_I1_001.fastq.gz",
            "upload_status": "validated"
        }
    ],
    "input_for": [
        "/analysis-sets/IGVFDS6299IJHG/"
    ],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "notes": "The readout 2e41e624-f6c7-4f0c-a07e-bc36cefdca39 was removed from this measurement set. This measurement set previously used Variant FlowFISH as a preferred_assay_title, but this enum is now removed. So it has been defaulted to Variant-EFFECTS.",
    "preferred_assay_title": "Variant-EFFECTS",
    "protocols": [
        "https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
    ],
    "release_timestamp": "2023-12-20T23:08:15.792663+00:00",
    "samples": [
        {
            "@id": "/in-vitro-systems/IGVFSM9682UDFK/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM9682UDFK",
            "aliases": [
                "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep2-FFrep2-BinD"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                {
                    "@id": "/construct-library-sets/IGVFDS2385DHZO/",
                    "accession": "IGVFDS2385DHZO",
                    "file_set_type": "guide library",
                    "status": "in progress",
                    "summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
                }
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0002796/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:Jurkat"
                    ],
                    "status": "released",
                    "summary": "Jurkat",
                    "term_name": "Jurkat"
                }
            ],
            "status": "released",
            "summary": "Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        }
    ],
    "schema_version": "23",
    "status": "released",
    "submitted_by": {
        "@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
        "title": "Michael Montgomery"
    },
    "submitted_files_timestamp": "2023-12-13T10:08:50.257279+00:00",
    "summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
    "targeted_genes": [
        {
            "@id": "/genes/ENSG00000108179/",
            "geneid": "ENSG00000108179",
            "name": "peptidylprolyl isomerase F",
            "status": "released",
            "symbol": "PPIF",
            "synonyms": [
                "rotamase F",
                "CYP3",
                "cyclophilin F",
                "mitochondrial cyclophilin",
                "peptidyl-prolyl cis-trans isomerase, mitochondrial",
                "CypD",
                "Cyp-D",
                "cyclophilin 3",
                "cyclophilin D",
                "CyP-M",
                "peptidyl-prolyl cis-trans isomerase F, mitochondrial",
                "PPIase F"
            ]
        }
    ],
    "uuid": "718b62e6-a01c-4a01-b802-98e4a09c0f68"
}