IGVFDS8782KHRJ

released
File Set Type
experimental data
Summary
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Assay Term
Targeted Genes
Sample Summaries
  • Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 26-50% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
Construct Library Sets
  • IGVFDS2385DHZO guide (sgRNA) library targeting sequence variants in a genomic locus
Protocols
Aliases
jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep1-FFrep2-BinB_AmpliconSequencing

Sequencing Results (Illumina)

48 items
fastq
reads
I2
1
AGTTGCTT:CAGT
2.0 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
CTATGCGT:CAGT
1.8 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
ATTATGTT:CAGT
2.0 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
CAGCGGTA:CAGT
9.5 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
CACATCCT:CAGT
1.8 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
AGTTGCTT:CAGT
9.6 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
CGCTATGT:CAGT
1.9 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
ATTGTCTG:CAGT
2.0 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
AGTTGCTT:CAGT
6.8 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
CTGTGGCG:CAGT
6.0 MB
Jesse Engreitz, Stanford
validated

Attribution