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INDEXED
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MeasurementSet
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IGVFDS8727LMYM
IGVFDS8727LMYM
released
Json view
File Set Type
experimental data
Summary
CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3
Assay Term
in vitro CRISPR screen using flow cytometry
Sample Summaries
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin B for 26-50% expression of NOS3) modified with a guide library
Construct Library Sets
IGVFDS8953DFJK
Guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3
Protocols
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
Aliases
jesse-engreitz:measurement_NOS3_CRISPRi_FF_R2_FFrep2_binB_PCR1_S138
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM5877PFFV
InVitroSystem
TeloHAEC
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin B for 26-50% expression of NOS3) modified with a guide library
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO3459DFTW
igvf:donor_of_teloHAEC
female
Homo sapiens
Auxiliary Datasets
1 item
Accession
Type
Summary
Files
Aliases
Lab
IGVFDS5941SDGS
AuxiliarySet
gRNA sequencing for CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3
IGVFFI2953ILAY
,
IGVFFI5283HKQE
,
IGVFFI3233KRJO
jesse-engreitz:auxiliary_NOS3_CRISPRi_FF_R2_FFrep2_binB_PCR1_S138
Jesse Engreitz, Stanford
Documents
1 item
Page
Description
Type
Download
Preview
experimental overview
experimental protocol
NOS3_VFF_design.pdf
Download NOS3_VFF_design.pdf
Download NOS3_VFF_design.pdf
Attribution
Award
HG011972
Principal Investigator(s)
Anshul Kundaje
,
Jesse Engreitz
,
Marlene Rabinovitch
,
Lars Steinmetz
,
Michael Bassik
,
Thomas Quertermous
,
Will Greenleaf
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford