{
"@context": "/terms/",
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"Item"
],
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],
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"term_name": "massively parallel reporter assay"
},
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{
"category": "missing sequence specification",
"detail": "Measurement set [IGVFDS8540VFIL](/measurement-sets/IGVFDS8540VFIL/) has sequence file(s): [IGVFFI8769NNTP](/sequence-files/IGVFFI8769NNTP/), [IGVFFI5392VALW](/sequence-files/IGVFFI5392VALW/), [IGVFFI2066NQJI](/sequence-files/IGVFFI2066NQJI/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
"level": 50,
"level_name": "NOT_COMPLIANT",
"name": "audit_missing_seqspec",
"path": "/measurement-sets/IGVFDS8540VFIL/"
},
{
"category": "missing sequence specification",
"detail": "Auxiliary set [IGVFDS8746LSXO](/auxiliary-sets/IGVFDS8746LSXO/) has sequence file(s): [IGVFFI1591CZNR](/sequence-files/IGVFFI1591CZNR/), [IGVFFI7164IWPZ](/sequence-files/IGVFFI7164IWPZ/), [IGVFFI8604OWOL](/sequence-files/IGVFFI8604OWOL/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
"level": 50,
"level_name": "NOT_COMPLIANT",
"name": "audit_missing_seqspec",
"path": "/auxiliary-sets/IGVFDS8746LSXO/"
}
]
},
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{
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],
"file_set_type": "quantification DNA barcode sequencing"
}
],
"award": {
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"component": "functional characterization",
"contact_pi": {
"@id": "/users/eb543cda-67b7-493c-84eb-187d0dac13ba/",
"title": "Jay Shendure"
},
"title": "Massively parallel characterization of variants and elements impacting transcriptional regulation in dynamic cellular systems"
},
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"creation_timestamp": "2023-12-08T23:54:10.667745+00:00",
"donors": [
{
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"accession": "IGVFDO3898MNLZ",
"aliases": [
"jay-shendure:WD44_donor"
],
"sex": "male",
"status": "released",
"taxa": "Mus musculus"
}
],
"externally_hosted": false,
"file_set_type": "experimental data",
"files": [
{
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"accession": "IGVFFI8769NNTP",
"aliases": [
"jay-shendure:bulkMPRA_devCRE_mEBs_rep2B_1_day4_RNA_R3"
],
"content_type": "reads",
"creation_timestamp": "2023-12-09T00:52:26.747617+00:00",
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"href": "/sequence-files/IGVFFI8769NNTP/@@download/IGVFFI8769NNTP.fastq.gz",
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"sequencing_platform": {
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"term_name": "Illumina NextSeq 500"
},
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"upload_status": "validated"
},
{
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"content_type": "reads",
"creation_timestamp": "2023-12-09T00:52:24.382084+00:00",
"file_format": "fastq",
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"href": "/sequence-files/IGVFFI5392VALW/@@download/IGVFFI5392VALW.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/09/f215a147-5f77-4ad6-a0a8-ce33beaad27b/IGVFFI5392VALW.fastq.gz",
"sequencing_platform": {
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"term_name": "Illumina NextSeq 500"
},
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"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI2066NQJI/",
"accession": "IGVFFI2066NQJI",
"aliases": [
"jay-shendure:bulkMPRA_devCRE_mEBs_rep2B_1_day4_RNA_R2"
],
"content_type": "reads",
"creation_timestamp": "2023-12-09T00:52:25.669199+00:00",
"file_format": "fastq",
"file_size": 219770165,
"href": "/sequence-files/IGVFFI2066NQJI/@@download/IGVFFI2066NQJI.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/09/b74a261a-7af1-48c8-8562-722039d8c68d/IGVFFI2066NQJI.fastq.gz",
"sequencing_platform": {
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"term_name": "Illumina NextSeq 500"
},
"submitted_file_name": "/net/shendure/vol8/projects/JBL/GEO_sc_rep_fastqs_nobackup/geo_submission_20221108/bulkMPRA_devCRE_mEBs/bulk_MPRA_mEB_series_mBC_rep2B1_d04_RNA_R2_001.fastq.gz",
"upload_status": "validated"
}
],
"input_for": [
"/analysis-sets/IGVFDS2622CKLA/"
],
"lab": {
"@id": "/labs/jay-shendure/",
"title": "Jay Shendure, UW"
},
"notes": "The readout b59a5259-3a53-482b-a7a5-6f401bdad220 was removed from this measurement set.",
"preferred_assay_title": "MPRA",
"protocols": [
"https://www.protocols.io/file/n92rb7r27.pdf"
],
"publications": [
{
"@id": "/publications/398edd7f-1ebb-40a0-a04b-450a2565667c/",
"publication_identifiers": [
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"PMID:38724692"
]
}
],
"release_timestamp": "2024-06-27T00:41:19.069221+00:00",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM3811HZXX/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM3811HZXX",
"aliases": [
"jay-shendure:dev_CRE_mEBs_rep2B_part1_bulk_day4"
],
"cell_fate_change_treatments": [
{
"@id": "/treatments/9acc7857-c9dd-4b4a-a78b-fdf10c8ae679/",
"purpose": "differentiation",
"summary": "Depletion of Leukemia inhibitory factor",
"treatment_type": "protein"
}
],
"classifications": [
"differentiated cell specimen"
],
"construct_library_sets": [
{
"@id": "/construct-library-sets/IGVFDS7655GRFI/",
"accession": "IGVFDS7655GRFI",
"file_set_type": "reporter library",
"summary": "Reporter library targeting accessible genome regions in many genomic loci"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0004038/",
"@type": [
"SampleTerm",
"OntologyTerm",
"Item"
],
"status": "released",
"summary": "mouse embryonic stem cell",
"term_name": "mouse embryonic stem cell"
}
],
"status": "released",
"summary": "mouse embryonic stem differentiated cell specimen induced to embryoid body for 4 days, male, Mus musculus WD44 transfected with a reporter library",
"targeted_sample_term": {
"@id": "/sample-terms/UBERON_0014374/",
"term_name": "embryoid body"
},
"taxa": "Mus musculus"
}
],
"schema_version": "22",
"status": "released",
"submitted_by": {
"@id": "/users/73f58e9f-8f5d-4fee-a7cd-dd63c0167e4b/",
"title": "Beth Martin"
},
"submitted_files_timestamp": "2023-12-09T00:52:20.581483+00:00",
"summary": "MPRA integrating a reporter library targeting accessible genome regions in many genomic loci",
"uuid": "5ede1c7b-cdb5-4838-b6ea-df1488a9ccdf"
}