IGVFDS8517JVOP

released

File Set Type

experimental data

Summary

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

KITLG

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Construct Library Sets

IGVFDS7846UISG guide (sgRNA) library targeting accessible genome regions in many genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF1-BinC-PCR1_S3

jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF1-BinC-PCR2_S9

jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF1-BinC-PCR3_S99

jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF1-BinC-PCR4_S105

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM2684MUBP

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS8917HRRE

AuxiliarySet

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFFI5868RORR, IGVFFI5595DRFH, IGVFFI9296GYRI, IGVFFI3826HBLM

jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF1-BinC-PCR1_S3
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF1-BinC-PCR2_S9
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF1-BinC-PCR3_S99
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF1-BinC-PCR4_S105

Jesse Engreitz, Stanford

IGVFDS9621FJCZ

AuxiliarySet

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

IGVFFI8500GKDJ