IGVFDS8445NSWZ

released

File Set Type

experimental data

Summary

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

KITLG

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Construct Library Sets

IGVFDS7846UISG guide (sgRNA) library targeting accessible genome regions in many genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF2-BinF-PCR1_S66

jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF2-BinF-PCR2_S72

jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF2-BinF-PCR3_S162

jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF2-BinF-PCR4_S168

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM4713PHAB

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS0078EAAP

AuxiliarySet

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFFI0574QGWX, IGVFFI7101MEEN, IGVFFI2836GXUE, IGVFFI8827QTFJ

jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin6hrs-FF2-BinF-PCR1_S66
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin6hrs-FF2-BinF-PCR2_S72
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin6hrs-FF2-BinF-PCR3_S162
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin6hrs-FF2-BinF-PCR4_S168

Jesse Engreitz, Stanford

IGVFDS8781ZUPM

AuxiliarySet

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

IGVFFI9594ERWN