{
"@context": "/terms/",
"@id": "/measurement-sets/IGVFDS8377HOWJ/",
"@type": [
"MeasurementSet",
"FileSet",
"Item"
],
"accession": "IGVFDS8377HOWJ",
"aliases": [
"jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep2-FFrep2-BinF_AmpliconSequencing"
],
"assay_term": {
"@id": "/assay-terms/OBI_0003661/",
"assay_slims": [
"CRISPR screens"
],
"status": "released",
"term_name": "in vitro CRISPR screen using flow cytometry"
},
"audit": {
"INTERNAL_ACTION": [
{
"category": "missing publication",
"detail": "Measurement set [IGVFDS8377HOWJ](/measurement-sets/IGVFDS8377HOWJ/) has no `publications`. Released and archived file sets are expected to be associated with a publication.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_file_set_missing_publication",
"path": "/measurement-sets/IGVFDS8377HOWJ/"
},
{
"category": "missing sequencing kit",
"detail": "Measurement set [IGVFDS8377HOWJ](/measurement-sets/IGVFDS8377HOWJ/) has sequence file(s) [IGVFFI0145AONP](/sequence-files/IGVFFI0145AONP/), [IGVFFI2223NBIY](/sequence-files/IGVFFI2223NBIY/), [IGVFFI8465NTRP](/sequence-files/IGVFFI8465NTRP/), [IGVFFI2202YHUR](/sequence-files/IGVFFI2202YHUR/), [IGVFFI3285YJPI](/sequence-files/IGVFFI3285YJPI/), [IGVFFI1870HAJX](/sequence-files/IGVFFI1870HAJX/), [IGVFFI0629YNMY](/sequence-files/IGVFFI0629YNMY/), [IGVFFI5778PVSW](/sequence-files/IGVFFI5778PVSW/) which lack specification of a `sequencing_kit`. Sequence files in a file set associated wtih bulk data should specify a sequencing kit.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_inconsistent_sequencing_kit",
"path": "/measurement-sets/IGVFDS8377HOWJ/"
},
{
"category": "missing sequence specification",
"detail": "Measurement set [IGVFDS8377HOWJ](/measurement-sets/IGVFDS8377HOWJ/) has sequence file(s): [IGVFFI0145AONP](/sequence-files/IGVFFI0145AONP/), [IGVFFI2223NBIY](/sequence-files/IGVFFI2223NBIY/), [IGVFFI8465NTRP](/sequence-files/IGVFFI8465NTRP/), [IGVFFI2202YHUR](/sequence-files/IGVFFI2202YHUR/), [IGVFFI3285YJPI](/sequence-files/IGVFFI3285YJPI/), [IGVFFI1870HAJX](/sequence-files/IGVFFI1870HAJX/), [IGVFFI0629YNMY](/sequence-files/IGVFFI0629YNMY/), [IGVFFI5778PVSW](/sequence-files/IGVFFI5778PVSW/) which do not have any `seqspecs`. Sequence files in a file set associated with bulk data are expected to link to a sequence specification file.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_missing_seqspec",
"path": "/measurement-sets/IGVFDS8377HOWJ/"
},
{
"category": "mismatched status",
"detail": "Released in vitro system [IGVFSM4295RBJG](/in-vitro-systems/IGVFSM4295RBJG/) has in progress subobject construct library set [IGVFDS6377KNCH](/construct-library-sets/IGVFDS6377KNCH/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/in-vitro-systems/IGVFSM4295RBJG/"
},
{
"category": "missing publication",
"detail": "In vitro system [IGVFSM4295RBJG](/in-vitro-systems/IGVFSM4295RBJG/) has no `publications`. Released and archived samples are expected to be associated with a publication.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_sample_missing_publication",
"path": "/in-vitro-systems/IGVFSM4295RBJG/"
}
],
"WARNING": [
{
"category": "missing auxiliary set",
"detail": "Measurement set [IGVFDS8377HOWJ](/measurement-sets/IGVFDS8377HOWJ/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
"level": 40,
"level_name": "WARNING",
"name": "audit_missing_auxiliary_set",
"path": "/measurement-sets/IGVFDS8377HOWJ/"
}
]
},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
"title": "Jesse Engreitz"
},
"title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
},
"construct_library_sets": [
{
"@id": "/construct-library-sets/IGVFDS6377KNCH/",
"accession": "IGVFDS6377KNCH",
"file_set_type": "guide library",
"status": "in progress",
"summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
}
],
"control_file_sets": [
{
"@id": "/measurement-sets/IGVFDS6385JXFZ/",
"accession": "IGVFDS6385JXFZ",
"aliases": [
"jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-THP1-PE2_Exp3_NegControl_AmpliconSequencing"
],
"status": "released"
}
],
"control_for": [],
"creation_timestamp": "2023-11-30T22:46:21.271326+00:00",
"donors": [
{
"@id": "/human-donors/IGVFDO7359LVPI/",
"accession": "IGVFDO7359LVPI",
"aliases": [
"igvf:donor_of_THP1"
],
"sex": "male",
"status": "released",
"taxa": "Homo sapiens"
}
],
"externally_hosted": false,
"file_set_type": "experimental data",
"files": [
{
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"accession": "IGVFFI0145AONP",
"aliases": [
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],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T18:52:31.563637+00:00",
"file_format": "fastq",
"file_size": 15503670,
"href": "/sequence-files/IGVFFI0145AONP/@@download/IGVFFI0145AONP.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/db021eb2-c09a-4a2a-9173-9e5b321f46b3/IGVFFI0145AONP.fastq.gz",
"sequencing_platform": {
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"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220228pPPIF-FFSample-8-D21-pPPIF-Amp-pPPIF-2-2-1-BinF_I1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI2223NBIY/",
"accession": "IGVFFI2223NBIY",
"aliases": [
"jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep2-FFrep2-BinF-PCRrep1_R2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T18:40:59.712296+00:00",
"file_format": "fastq",
"file_size": 75915443,
"href": "/sequence-files/IGVFFI2223NBIY/@@download/IGVFFI2223NBIY.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/6dc01308-13e4-4667-a609-812572b7ec0e/IGVFFI2223NBIY.fastq.gz",
"sequencing_platform": {
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"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220228pPPIF-FFSample-8-D21-pPPIF-Amp-pPPIF-2-2-1-BinF_R2_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI8465NTRP/",
"accession": "IGVFFI8465NTRP",
"aliases": [
"jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep2-FFrep2-BinF-PCRrep2_R2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T18:46:57.780526+00:00",
"file_format": "fastq",
"file_size": 68958866,
"href": "/sequence-files/IGVFFI8465NTRP/@@download/IGVFFI8465NTRP.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/8d636d29-632c-405a-aad8-ca1507fcd876/IGVFFI8465NTRP.fastq.gz",
"sequencing_platform": {
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"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220228pPPIF-FFSample-8-D21-pPPIF-Amp-pPPIF-2-2-2-BinF_R2_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI2202YHUR/",
"accession": "IGVFFI2202YHUR",
"aliases": [
"jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep2-FFrep2-BinF-PCRrep2_R1"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T18:34:55.815348+00:00",
"file_format": "fastq",
"file_size": 38082722,
"href": "/sequence-files/IGVFFI2202YHUR/@@download/IGVFFI2202YHUR.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/3a07293e-d58e-46c1-9858-eb9adc5a177a/IGVFFI2202YHUR.fastq.gz",
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"status": "released",
"term_name": "Illumina NextSeq 550"
},
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"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI3285YJPI/",
"accession": "IGVFFI3285YJPI",
"aliases": [
"jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep2-FFrep2-BinF-PCRrep2_I2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T19:09:18.611576+00:00",
"file_format": "fastq",
"file_size": 13123820,
"href": "/sequence-files/IGVFFI3285YJPI/@@download/IGVFFI3285YJPI.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/e5c0f0b6-49ff-401d-baa3-d6ee72a0491c/IGVFFI3285YJPI.fastq.gz",
"sequencing_platform": {
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"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220228pPPIF-FFSample-8-D21-pPPIF-Amp-pPPIF-2-2-2-BinF_I2_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI1870HAJX/",
"accession": "IGVFFI1870HAJX",
"aliases": [
"jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep2-FFrep2-BinF-PCRrep1_R1"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T18:29:15.302696+00:00",
"file_format": "fastq",
"file_size": 41981587,
"href": "/sequence-files/IGVFFI1870HAJX/@@download/IGVFFI1870HAJX.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/1718c4b6-d123-42bc-b8cc-7eb75b519128/IGVFFI1870HAJX.fastq.gz",
"sequencing_platform": {
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"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220228pPPIF-FFSample-8-D21-pPPIF-Amp-pPPIF-2-2-1-BinF_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI0629YNMY/",
"accession": "IGVFFI0629YNMY",
"aliases": [
"jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep2-FFrep2-BinF-PCRrep2_I1"
],
"content_type": "reads",
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"creation_timestamp": "2023-12-05T18:58:11.318458+00:00",
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"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220228pPPIF-FFSample-8-D21-pPPIF-Amp-pPPIF-2-2-2-BinF_I1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI5778PVSW/",
"accession": "IGVFFI5778PVSW",
"aliases": [
"jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep2-FFrep2-BinF-PCRrep1_I2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T19:03:43.143314+00:00",
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"sequencing_platform": {
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"status": "released",
"term_name": "Illumina NextSeq 550"
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"submitted_file_name": "220228pPPIF-FFSample-8-D21-pPPIF-Amp-pPPIF-2-2-1-BinF_I2_001.fastq.gz",
"upload_status": "validated"
}
],
"input_for": [
"/analysis-sets/IGVFDS5056OAGR/"
],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"notes": "The readout 2e41e624-f6c7-4f0c-a07e-bc36cefdca39 was removed from this measurement set. This measurement set previously used Variant FlowFISH as a preferred_assay_title, but this enum is now removed. So it has been defaulted to Variant-EFFECTS.",
"preferred_assay_title": "Variant-EFFECTS",
"protocols": [
"https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
],
"release_timestamp": "2023-12-20T23:09:38.315537+00:00",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM4295RBJG/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM4295RBJG",
"aliases": [
"jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep2-FFrep2-BinF"
],
"classifications": [
"cell line"
],
"institutional_certificates": [],
"modifications": [
{
"@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
"modality": "prime editing",
"status": "released"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0001253/",
"@type": [
"SampleTerm",
"OntologyTerm",
"Item"
],
"aliases": [
"igvf:THP-1"
],
"status": "released",
"summary": "THP-1",
"term_name": "THP-1"
}
],
"status": "released",
"summary": "Homo sapiens (male) THP-1 cell line (sorting details: FACS bin for 90-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library",
"taxa": "Homo sapiens"
}
],
"schema_version": "26",
"status": "released",
"submitted_by": {
"@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
"title": "Michael Montgomery"
},
"submitted_files_timestamp": "2023-12-05T18:29:15.302696+00:00",
"summary": "CRISPR prime editing Variant-EFFECTS sorted on the expression of PPIF integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
"targeted_genes": [
{
"@id": "/genes/ENSG00000108179/",
"geneid": "ENSG00000108179",
"name": "peptidylprolyl isomerase F",
"status": "released",
"symbol": "PPIF",
"synonyms": [
"PPIase F",
"peptidylprolyl isomerase F",
"Cyp-D",
"peptidyl-prolyl cis-trans isomerase F, mitochondrial",
"PPIF",
"CyP-M",
"cyclophilin F",
"peptidyl-prolyl cis-trans isomerase, mitochondrial",
"cyclophilin 3",
"cyclophilin D",
"rotamase F",
"mitochondrial cyclophilin",
"CypD",
"CYP3"
]
}
],
"uuid": "ae66c553-951f-4ff8-88cd-7a77404081fe"
}