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IGVFDS8329NAOV

released
File Set Type
experimental data
Summary
CRISPR interference FlowFISH screen sorted on the expression of TLNRD1 integrating a guide (sgRNA) library targeting candidate cis-regulatory elements in a genomic locus
Assay Term
in vitro CRISPR screen using flow cytometry
Targeted Genes
Sample Summaries
  • Homo sapiens (female) TeloHAEC cell line (sorting details: FACS bin for 26-50% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
Protocols
Aliases
jesse-engreitz:measurement_set_eRZ10_teloHAEC_TLNRD1_enhancer_CRISPRi_FlowFISH_Rep1_bin2
Publications
Attribution
Jesse Engreitz, Stanford

Sequencing Results (Illumina)

1 item
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Read Names
Associated seqspec Files
Sequence Specification Document
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
fastq
reads
R1
1
70 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Associated Construct Library Sets
Summary
InVitroSystem
Homo sapiens (female) TeloHAEC cell line (sorting details: FACS bin for 26-50% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_teloHAEC
female
Homo sapiens

Associated Construct Library Sets

1 item
Accession
Summary
Library Design Files
guide (sgRNA) library targeting candidate cis-regulatory elements in a genomic locus

File Sets Using This Measurement Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
CRISPR interference FlowFISH screen targeting TLNRD1 integrating a guide (sgRNA) library targeting candidate cis-regulatory elements in a genomic locus
  • jesse-engreitz:analysis_set_eRZ10_teloHAEC_TLNRD1_enhancer_CRISPRi_FlowFISH_analysis
Jesse Engreitz, Stanford