IGVFDS8267KGLQ

released

File Set Type

experimental data

Summary

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

MYC

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Construct Library Sets

IGVFDS7857SYCK guide (sgRNA) library targeting accessible genome regions in many genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR1_Rep1_S139

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR1_Rep2_S194

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR2_Rep1_S145

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR2_Rep2_S200

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR3_Rep1_S151

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR3_Rep2_S206

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR4_Rep1_S157

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR4_Rep2_S212

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM2548FJWP

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS1748WPZO

AuxiliarySet

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFFI7918JLEC, IGVFFI4124LZQS, IGVFFI1556PTKF, IGVFFI6201YBUU, IGVFFI0138SEGI, IGVFFI7692ITME, IGVFFI7499BCPY, IGVFFI4194ZWJO

jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR1_Rep1_S139
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR1_Rep2_S194
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR2_Rep1_S145
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR2_Rep2_S200
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR3_Rep1_S151
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR3_Rep2_S206
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR4_Rep1_S157
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR4_Rep2_S212

Jesse Engreitz, Stanford

IGVFDS5394TNHK

AuxiliarySet

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

IGVFFI9629PGSJ