{
"@context": "/terms/",
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],
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],
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"term_name": "in vitro CRISPR screen using flow cytometry"
},
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"INTERNAL_ACTION": [
{
"category": "missing sequencing kit",
"detail": "Measurement set [IGVFDS8217QYTZ](/measurement-sets/IGVFDS8217QYTZ/) has sequence file(s) [IGVFFI2217GEXV](/sequence-files/IGVFFI2217GEXV/), [IGVFFI4171LDUJ](/sequence-files/IGVFFI4171LDUJ/), [IGVFFI6750EGCQ](/sequence-files/IGVFFI6750EGCQ/), [IGVFFI4720REHO](/sequence-files/IGVFFI4720REHO/), [IGVFFI3295KMEK](/sequence-files/IGVFFI3295KMEK/), [IGVFFI7368BEKM](/sequence-files/IGVFFI7368BEKM/), [IGVFFI6606LPVT](/sequence-files/IGVFFI6606LPVT/), [IGVFFI6575ODQJ](/sequence-files/IGVFFI6575ODQJ/), [IGVFFI0639RCJW](/sequence-files/IGVFFI0639RCJW/), [IGVFFI8817IBGO](/sequence-files/IGVFFI8817IBGO/), [IGVFFI0082QOVU](/sequence-files/IGVFFI0082QOVU/), [IGVFFI4251UYMH](/sequence-files/IGVFFI4251UYMH/), [IGVFFI5684RZTU](/sequence-files/IGVFFI5684RZTU/), [IGVFFI1661NQPT](/sequence-files/IGVFFI1661NQPT/), [IGVFFI0277FTFT](/sequence-files/IGVFFI0277FTFT/), [IGVFFI3344AXUV](/sequence-files/IGVFFI3344AXUV/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_inconsistent_sequencing_kit",
"path": "/measurement-sets/IGVFDS8217QYTZ/"
},
{
"category": "missing sequence specification",
"detail": "Measurement set [IGVFDS8217QYTZ](/measurement-sets/IGVFDS8217QYTZ/) has sequence file(s): [IGVFFI2217GEXV](/sequence-files/IGVFFI2217GEXV/), [IGVFFI4171LDUJ](/sequence-files/IGVFFI4171LDUJ/), [IGVFFI6750EGCQ](/sequence-files/IGVFFI6750EGCQ/), [IGVFFI4720REHO](/sequence-files/IGVFFI4720REHO/), [IGVFFI3295KMEK](/sequence-files/IGVFFI3295KMEK/), [IGVFFI7368BEKM](/sequence-files/IGVFFI7368BEKM/), [IGVFFI6606LPVT](/sequence-files/IGVFFI6606LPVT/), [IGVFFI6575ODQJ](/sequence-files/IGVFFI6575ODQJ/), [IGVFFI0639RCJW](/sequence-files/IGVFFI0639RCJW/), [IGVFFI8817IBGO](/sequence-files/IGVFFI8817IBGO/), [IGVFFI0082QOVU](/sequence-files/IGVFFI0082QOVU/), [IGVFFI4251UYMH](/sequence-files/IGVFFI4251UYMH/), [IGVFFI5684RZTU](/sequence-files/IGVFFI5684RZTU/), [IGVFFI1661NQPT](/sequence-files/IGVFFI1661NQPT/), [IGVFFI0277FTFT](/sequence-files/IGVFFI0277FTFT/), [IGVFFI3344AXUV](/sequence-files/IGVFFI3344AXUV/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_missing_seqspec",
"path": "/measurement-sets/IGVFDS8217QYTZ/"
}
],
"WARNING": [
{
"category": "missing auxiliary set",
"detail": "Measurement set [IGVFDS8217QYTZ](/measurement-sets/IGVFDS8217QYTZ/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
"level": 40,
"level_name": "WARNING",
"name": "audit_missing_auxiliary_set",
"path": "/measurement-sets/IGVFDS8217QYTZ/"
}
]
},
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"submitted_file_name": "240908-S029-5splice-THP1-5splicesite_C12-Amp_PPIF_5splicesite-BioRep2-FFrep2-BinC-PCRrep3_S949_I1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI3344AXUV/",
"accession": "IGVFFI3344AXUV",
"aliases": [
"jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep2-FFrep2-BinC-PCRrep3_I2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-12-12T00:51:19.957341+00:00",
"file_format": "fastq",
"file_size": 11152214,
"href": "/sequence-files/IGVFFI3344AXUV/@@download/IGVFFI3344AXUV.fastq.gz",
"s3_uri": "s3://igvf-files/2024/12/12/3c8310fc-73cd-4933-9794-2ae86213d299/IGVFFI3344AXUV.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0022840/",
"status": "released",
"term_name": "Illumina NovaSeq X"
},
"status": "preview",
"submitted_file_name": "240908-S029-5splice-THP1-5splicesite_C12-Amp_PPIF_5splicesite-BioRep2-FFrep2-BinC-PCRrep3_S949_I2_001.fastq.gz",
"upload_status": "validated"
}
],
"input_for": [
"/analysis-sets/IGVFDS8174BPPS/"
],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"preferred_assay_title": "Variant-EFFECTS",
"protocols": [
"https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
],
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM2418RCAK/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM2418RCAK",
"aliases": [
"jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep2-FFrep2-BinC"
],
"classifications": [
"cell line"
],
"construct_library_sets": [
{
"@id": "/construct-library-sets/IGVFDS6555EOZU/",
"accession": "IGVFDS6555EOZU",
"file_set_type": "guide library",
"status": "preview",
"summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
}
],
"modifications": [
{
"@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
"modality": "prime editing",
"status": "released"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0001253/",
"@type": [
"SampleTerm",
"OntologyTerm",
"Item"
],
"aliases": [
"igvf:THP-1"
],
"status": "released",
"summary": "THP-1",
"term_name": "THP-1"
}
],
"status": "preview",
"summary": "THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
"taxa": "Homo sapiens"
}
],
"schema_version": "23",
"status": "preview",
"submitted_by": {
"@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
"title": "Michael Montgomery"
},
"submitted_files_timestamp": "2024-12-11T16:49:57.097104+00:00",
"summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
"targeted_genes": [
{
"@id": "/genes/ENSG00000108179/",
"geneid": "ENSG00000108179",
"name": "peptidylprolyl isomerase F",
"status": "released",
"symbol": "PPIF",
"synonyms": [
"rotamase F",
"CYP3",
"cyclophilin F",
"mitochondrial cyclophilin",
"peptidyl-prolyl cis-trans isomerase, mitochondrial",
"CypD",
"Cyp-D",
"cyclophilin 3",
"cyclophilin D",
"CyP-M",
"peptidyl-prolyl cis-trans isomerase F, mitochondrial",
"PPIase F"
]
}
],
"uuid": "d1b02e88-7dfc-49f2-86ac-17d03e469a66"
}