IGVFDS7960YGMN

released

File Set Type

experimental data

Summary

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

KITLG

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with a guide library

Construct Library Sets

IGVFDS7846UISG Guide (sgRNA) library targeting accessible genome regions in many genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinD-PCR1_S52

jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinD-PCR2_S58

jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinD-PCR3_S148

jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinD-PCR4_S154

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM8244VKAL

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS2452BXCK

AuxiliarySet

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFFI3469NFLH, IGVFFI6997WQGE, IGVFFI4752YNUH, IGVFFI5272HZEO

jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin6hrs-FF1-BinD-PCR1_S52
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin6hrs-FF1-BinD-PCR2_S58
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin6hrs-FF1-BinD-PCR3_S148
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin6hrs-FF1-BinD-PCR4_S154

Jesse Engreitz, Stanford

IGVFDS4521OAKZ

AuxiliarySet

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

IGVFFI3484IZUF