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IGVFDS7733OTCA

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File Set Type
experimental data
Summary
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Assay Term
in vitro CRISPR screen using single-cell RNA-seq
Sample Summaries
  • GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW279), male, Homo sapiens modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
Construct Library Sets
  • IGVFDS2302LCEO guide (sgRNA) library targeting accessible genome regions in many genomic loci
Protocols
Aliases
gary-hon:TF_Perturb_Seq_Pilot_Lenti_CM_LW279
Submitter Comment
Transcriptiome for LW279

Sequencing Results (Illumina)

16 items
fastq
reads
I2
1
AAC7VLGHV
1
75 MB
Gary Hon, UT
validated
fastq
reads
R1
1
AAC7VLGHV
2
193 MB
Gary Hon, UT
validated
fastq
reads
R1
1
AAC7VLGHV
1
194 MB
Gary Hon, UT
validated
fastq
reads
I1
1
AAC5W2WHV
2
667 MB
Gary Hon, UT
validated
fastq
reads
R2
1
AAC5W2WHV
1
6.7 GB
Gary Hon, UT
validated
fastq
reads
I1
1
AAC7VLGHV
2
64 MB
Gary Hon, UT
validated
fastq
reads
I2
1
AAC5W2WHV
1
752 MB
Gary Hon, UT
validated
fastq
reads
R1
1
AAC5W2WHV
2
2.6 GB
Gary Hon, UT
validated
fastq
reads
R2
1
AAC7VLGHV
2
452 MB
Gary Hon, UT
validated
fastq
reads
I2
1
AAC7VLGHV
2
73 MB
Gary Hon, UT
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW279), male, Homo sapiens modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_WTC11
male
Homo sapiens

File Sets Using This Measurement Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
interference Perturb-seq: filtered feature barcode matrix, raw feature barcode matrix, sparse gene count matrix, unfiltered global differential expression, unfiltered local differential expression
  • gary-hon:TF_Perturb_Seq_Pilot_Lenti_CM_PrimaryAnalysis
Gary Hon, UT

Auxiliary Datasets

2 items
Files
AuxiliarySet
gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • gary-hon:TF_Perturb_Seq_Pilot_Lenti_CM_sgRNA_LW279
Gary Hon, UT
AuxiliarySet
cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • gary-hon:TF_Perturb_Seq_Pilot_Lenti_CM_HTO_LW279
Gary Hon, UT

Attribution

Award
HG011996
Principal Investigator(s)
Contact P.I.
Gary Hon
Lab
Gary Hon, UT