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IGVFDS7729SUIF
IGVFDS7729SUIF
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This object is in preview status. Metadata is subject to change until this object is released.
File Set Type
experimental data
Summary
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci
Assay Term
in vitro CRISPR screen using single-cell RNA-seq
Sample Summaries
Homo sapiens (female) TeloHAEC cell line modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
Protocols
https://www.protocols.io/view/systematic-perturb-seq-to-discover-endothelial-cel-261ged73yv47/v1
Aliases
jesse-engreitz:measurement_set-scRNAseq_2kG_2PMDox2_4
Publications
Convergence of coronary artery disease genes onto endothelial cell programs
Attribution
Jesse Engreitz, Stanford
(
HG011972
—
Jesse Engreitz
)
Sequencing Results (Illumina)
15 items
1
2
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Read Names
Associated seqspec Files
Sequence Specification Document
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI0137BUSM
fastq
reads
I1
2
Illumina NovaSeq 6000
989 MB
Jesse Engreitz, Stanford
validated
IGVFFI0253SJFQ
fastq
reads
I1
4
Illumina NovaSeq 6000
898 MB
Jesse Engreitz, Stanford
validated
IGVFFI0468IETI
fastq
reads
R2
4
Illumina NovaSeq 6000
4.5 GB
Jesse Engreitz, Stanford
validated
IGVFFI0469KBZO
fastq
reads
R2
2
Illumina NovaSeq 6000
4.9 GB
Jesse Engreitz, Stanford
validated
IGVFFI1096ZNTL
fastq
reads
R2
1
Illumina NovaSeq 6000
5.0 GB
Jesse Engreitz, Stanford
validated
IGVFFI2446JWJN
fastq
reads
I1
5
Illumina NovaSeq 6000
2.6 GB
Jesse Engreitz, Stanford
validated
IGVFFI2453FDMJ
fastq
reads
R1
3
Illumina NovaSeq 6000
3.6 GB
Jesse Engreitz, Stanford
validated
IGVFFI3194TNMB
fastq
reads
I1
3
Illumina NovaSeq 6000
990 MB
Jesse Engreitz, Stanford
validated
IGVFFI4040TILV
fastq
reads
R1
5
Illumina NovaSeq 6000
9.1 GB
Jesse Engreitz, Stanford
validated
IGVFFI4525LELV
fastq
reads
R2
5
Illumina NovaSeq 6000
12 GB
Jesse Engreitz, Stanford
validated
Samples
1 item
Accession
Type
Sample Terms
Associated Construct Library Sets
Summary
IGVFSM7987ARAX
InVitroSystem
TeloHAEC
IGVFDS1298TUYG
Homo sapiens (female) TeloHAEC cell line modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO3459DFTW
igvf:donor_of_teloHAEC
female
Homo sapiens
Associated Construct Library Sets
1 item
Accession
Summary
Library Design Files
IGVFDS1298TUYG
guide (sgRNA) library targeting genes in many genomic loci
IGVFFI9754AGFB
File Sets Using This Measurement Set as an Input
1 item
Accession
Type
Summary
Aliases
Lab
IGVFDS7943IIWZ
AnalysisSet
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci
jesse-engreitz:analysis_set_TeloHAEC_Perturb-seq_2kG
Jesse Engreitz, Stanford
Auxiliary Datasets
1 item
Accession
Type
Summary
Files
Aliases
Lab
IGVFDS0515YYBH
AuxiliarySet
gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci
IGVFFI1080VUNA
,
IGVFFI2584CVDW
,
IGVFFI1429WWYJ
,
IGVFFI0801SADR
jesse-engreitz:auxiliary_sets-scRNAseq_2kG_2PMDox2_4
Jesse Engreitz, Stanford