IGVFDS7687LEGW

released

File Set Type

experimental data

Summary

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

ITPRID2

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of SSFA2) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Construct Library Sets

IGVFDS5413RKIA guide (sgRNA) library targeting accessible genome regions in many genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinF-PCR1-Rep1_S30

jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinF-PCR2-Rep1_S36

jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinF-PCR3-Rep1_S84

jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinF-PCR4-Rep1_S90

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM6010ONZP

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of SSFA2) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS4138DUVP

AuxiliarySet

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFFI5487DYXT, IGVFFI3423LGND, IGVFFI1411ZGTO, IGVFFI0044PLIJ

jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin6hrs-FF1-BinF-PCR1-Rep1_S30
jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin6hrs-FF1-BinF-PCR2-Rep1_S36
jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin6hrs-FF1-BinF-PCR3-Rep1_S84
jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin6hrs-FF1-BinF-PCR4-Rep1_S90

Jesse Engreitz, Stanford

IGVFDS6020CDHF

AuxiliarySet

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

IGVFFI8558XTCT