IGVFDS7600PZYR

preview

This object is in preview status. Metadata is subject to change until this object is released.

File Set Type

experimental data

Summary

CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci

Assay Term

in vitro CRISPR screen using single-cell RNA-seq

Sample Summaries

Homo sapiens (female) TeloHAEC cell line modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library

Protocols

https://www.protocols.io/view/systematic-perturb-seq-to-discover-endothelial-cel-261ged73yv47/v1

Aliases

jesse-engreitz:measurement_set-scRNAseq_2kG_2PMDox2_1

Publications

Convergence of coronary artery disease genes onto endothelial cell programs

Attribution

Jesse Engreitz, Stanford

(

HG011972 — Jesse Engreitz

)

Sequencing Results (Illumina)

15 items

Read Names

IGVFFI1024CKHO

fastq

reads

Illumina NovaSeq 6000

4.8 GB

Jesse Engreitz, Stanford

validated

IGVFFI1492IMBA

fastq

reads

Illumina NovaSeq 6000

5.0 GB

Jesse Engreitz, Stanford

validated

IGVFFI2157LCVI

fastq

reads

Illumina NovaSeq 6000

1.0 GB

Jesse Engreitz, Stanford

validated

IGVFFI2426GZJV

fastq

reads

Illumina NovaSeq 6000

3.5 GB

Jesse Engreitz, Stanford

validated

IGVFFI2912EVSN

fastq

reads

Illumina NovaSeq 6000

3.5 GB

Jesse Engreitz, Stanford

validated

IGVFFI4487DHRU

fastq

reads

Illumina NovaSeq 6000

3.0 GB

Jesse Engreitz, Stanford

validated

IGVFFI4544QBUW

fastq

reads

Illumina NovaSeq 6000

824 MB

Jesse Engreitz, Stanford

validated

IGVFFI4775GVGW

fastq

reads

Illumina NovaSeq 6000

952 MB

Jesse Engreitz, Stanford

validated

IGVFFI5607YHVQ

fastq

reads

Illumina NovaSeq 6000

3.6 GB

Jesse Engreitz, Stanford

validated

IGVFFI6125WLFU

fastq

reads

Illumina NovaSeq 6000

2.9 GB

Jesse Engreitz, Stanford

validated

Samples

1 item

Accession

Type

Sample Terms

Associated Construct Library Sets

Summary

IGVFSM0862BZMH

InVitroSystem

TeloHAEC

IGVFDS1298TUYG

Homo sapiens (female) TeloHAEC cell line modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO3459DFTW

igvf:donor_of_teloHAEC

female

Homo sapiens

Associated Construct Library Sets

1 item

Accession

Summary

Library Design Files

IGVFDS1298TUYG

guide (sgRNA) library targeting genes in many genomic loci

IGVFFI9754AGFB

File Sets Using This Measurement Set as an Input

1 item

Accession

Type

Summary

Aliases

Lab

IGVFDS7943IIWZ

AnalysisSet

CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci

jesse-engreitz:analysis_set_TeloHAEC_Perturb-seq_2kG

Jesse Engreitz, Stanford

Auxiliary Datasets

1 item

Accession

Type

Summary

Files

Aliases

Lab

IGVFDS4784DDTU

AuxiliarySet

gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci

IGVFFI4092GRZQ, IGVFFI1378RGUV, IGVFFI7575DTDH, IGVFFI4619DNJG

jesse-engreitz:auxiliary_sets-scRNAseq_2kG_2PMDox2_1

Jesse Engreitz, Stanford