{
"@context": "/terms/",
"@id": "/measurement-sets/IGVFDS7547SYUP/",
"@type": [
"MeasurementSet",
"FileSet",
"Item"
],
"accession": "IGVFDS7547SYUP",
"aliases": [
"jay-shendure:dev_CRE_mEBs_repA_bulk_RNA_day8_reads"
],
"assay_term": {
"@id": "/assay-terms/OBI_0002675/",
"assay_slims": [
"reporter"
],
"status": "released",
"term_name": "massively parallel reporter assay"
},
"audit": {},
"auxiliary_sets": [
{
"@id": "/auxiliary-sets/IGVFDS1683YYFI/",
"accession": "IGVFDS1683YYFI",
"aliases": [
"jay-shendure:dev_CRE_mEBs_repA_bulk_DNA_day8_reads"
],
"file_set_type": "quantification DNA barcode sequencing",
"status": "released"
}
],
"award": {
"@id": "/awards/HG011966/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/eb543cda-67b7-493c-84eb-187d0dac13ba/",
"title": "Jay Shendure"
},
"title": "Massively parallel characterization of variants and elements impacting transcriptional regulation in dynamic cellular systems"
},
"construct_library_sets": [
{
"@id": "/construct-library-sets/IGVFDS7655GRFI/",
"accession": "IGVFDS7655GRFI",
"file_set_type": "reporter library",
"integrated_content_files": [
{
"@id": "/tabular-files/IGVFFI8904VCYX/",
"accession": "IGVFFI8904VCYX",
"content_type": "MPRA sequence designs",
"status": "released",
"summary": "MPRA sequence designs"
}
],
"status": "released",
"summary": "reporter library targeting accessible genome regions in many genomic loci"
}
],
"control_for": [],
"creation_timestamp": "2023-12-08T23:53:54.817824+00:00",
"donors": [
{
"@id": "/rodent-donors/IGVFDO3898MNLZ/",
"accession": "IGVFDO3898MNLZ",
"aliases": [
"jay-shendure:WD44_donor"
],
"sex": "male",
"status": "released",
"strain_background": "C57BL/6J (B6)",
"taxa": "Mus musculus"
}
],
"externally_hosted": false,
"file_set_type": "experimental data",
"files": [
{
"@id": "/sequence-files/IGVFFI1640ZLRF/",
"accession": "IGVFFI1640ZLRF",
"aliases": [
"jay-shendure:bulkMPRA_devCRE_mEBs_repA_day8_RNA_R1"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-09T00:54:40.528725+00:00",
"file_format": "fastq",
"file_size": 228141341,
"href": "/sequence-files/IGVFFI1640ZLRF/@@download/IGVFFI1640ZLRF.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/09/bff3ffc6-eef2-4e6c-9c4e-bf9dbdc62aaa/IGVFFI1640ZLRF.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0009173/",
"status": "released",
"term_name": "Illumina NextSeq 500"
},
"status": "released",
"submitted_file_name": "/net/shendure/vol8/projects/JBL/GEO_sc_rep_fastqs_nobackup/geo_submission_20221108/bulkMPRA_devCRE_mEBs/bulk_MPRA_mEB_series_mBC_repA_d08_RNA_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI5663GQLI/",
"accession": "IGVFFI5663GQLI",
"aliases": [
"jay-shendure:bulkMPRA_devCRE_mEBs_repA_day8_RNA_R2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-09T00:54:42.337830+00:00",
"file_format": "fastq",
"file_size": 114024917,
"href": "/sequence-files/IGVFFI5663GQLI/@@download/IGVFFI5663GQLI.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/09/960ad8ea-e90d-4e70-a7cf-2a45dbb5badb/IGVFFI5663GQLI.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0009173/",
"status": "released",
"term_name": "Illumina NextSeq 500"
},
"status": "released",
"submitted_file_name": "/net/shendure/vol8/projects/JBL/GEO_sc_rep_fastqs_nobackup/geo_submission_20221108/bulkMPRA_devCRE_mEBs/bulk_MPRA_mEB_series_mBC_repA_d08_RNA_R2_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI9283XQMD/",
"accession": "IGVFFI9283XQMD",
"aliases": [
"jay-shendure:bulkMPRA_devCRE_mEBs_repA_day8_RNA_R3"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-09T00:54:43.651448+00:00",
"file_format": "fastq",
"file_size": 328069543,
"href": "/sequence-files/IGVFFI9283XQMD/@@download/IGVFFI9283XQMD.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/09/cb133e0b-ac2d-4363-bc29-921ecd007306/IGVFFI9283XQMD.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0009173/",
"status": "released",
"term_name": "Illumina NextSeq 500"
},
"status": "released",
"submitted_file_name": "/net/shendure/vol8/projects/JBL/GEO_sc_rep_fastqs_nobackup/geo_submission_20221108/bulkMPRA_devCRE_mEBs/bulk_MPRA_mEB_series_mBC_repA_d08_RNA_R3_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/configuration-files/IGVFFI3631ESRA/",
"accession": "IGVFFI3631ESRA",
"aliases": [
"jay-shendure:seqspec_of_dev_CRE_mEBs_repA_bulk_RNA_day8_reads"
],
"content_type": "seqspec",
"creation_timestamp": "2025-04-03T22:49:55.693649+00:00",
"file_format": "yaml",
"file_size": 1152,
"href": "/configuration-files/IGVFFI3631ESRA/@@download/IGVFFI3631ESRA.yaml.gz",
"s3_uri": "s3://igvf-files/2025/04/03/e9801ecf-6166-4284-9444-983fc39d2531/IGVFFI3631ESRA.yaml.gz",
"status": "in progress",
"submitted_file_name": "/net/shendure/vol1/home/martin91/IGVF/forupload/seqspecs/scQers-bulkMPRA/scQers_IGVFDS7547SYUP_seqspec_v3.yaml.gz",
"upload_status": "validated"
}
],
"input_for": [
"/analysis-sets/IGVFDS2622CKLA/"
],
"lab": {
"@id": "/labs/jay-shendure/",
"title": "Jay Shendure, UW"
},
"notes": "The readout b59a5259-3a53-482b-a7a5-6f401bdad220 was removed from this measurement set.",
"preferred_assay_title": "MPRA",
"protocols": [
"https://www.protocols.io/file/n92rb7r27.pdf"
],
"publications": [
{
"@id": "/publications/398edd7f-1ebb-40a0-a04b-450a2565667c/",
"publication_identifiers": [
"doi:10.1038/s41592-024-02260-3",
"PMID:38724692"
],
"status": "released"
}
],
"release_timestamp": "2024-06-27T00:41:13.628611+00:00",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM5894TCUC/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM5894TCUC",
"aliases": [
"jay-shendure:dev_CRE_mEBs_repA_bulk_day8"
],
"classifications": [
"differentiated cell specimen"
],
"institutional_certificates": [],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0004038/",
"@type": [
"SampleTerm",
"OntologyTerm",
"Item"
],
"status": "released",
"summary": "mouse embryonic stem cell",
"term_name": "mouse embryonic stem cell"
}
],
"status": "released",
"summary": "Mus musculus WD44 (male) mouse embryonic stem differentiated cell specimen induced to embryoid body for 8 days transfected with a reporter library",
"targeted_sample_term": {
"@id": "/sample-terms/UBERON_0014374/",
"status": "released",
"term_name": "embryoid body"
},
"taxa": "Mus musculus"
}
],
"schema_version": "26",
"status": "released",
"submitted_by": {
"@id": "/users/73f58e9f-8f5d-4fee-a7cd-dd63c0167e4b/",
"title": "Beth Martin"
},
"submitted_files_timestamp": "2023-12-09T00:54:35.127306+00:00",
"summary": "MPRA integrating a reporter library targeting accessible genome regions in many genomic loci",
"uuid": "9968169e-5ab5-4955-b905-565ff7204c53"
}