{
"@context": "/terms/",
"@id": "/measurement-sets/IGVFDS7182DGDS/",
"@type": [
"MeasurementSet",
"FileSet",
"Item"
],
"accession": "IGVFDS7182DGDS",
"aliases": [
"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR1_Rep1_S111",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR1_Rep2_S166",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR2_Rep1_S117",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR2_Rep2_S172",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR3_Rep1_S123",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR3_Rep2_S178",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR4_Rep1_S129",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR4_Rep2_S184"
],
"assay_term": {
"@id": "/assay-terms/OBI_0003661/",
"assay_slims": [
"CRISPR screens"
],
"status": "released",
"term_name": "in vitro CRISPR screen using flow cytometry"
},
"audit": {
"INTERNAL_ACTION": [
{
"category": "missing publication",
"detail": "Measurement set [IGVFDS7182DGDS](/measurement-sets/IGVFDS7182DGDS/) has no `publications`. Released and archived file sets are expected to be associated with a publication.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_file_set_missing_publication",
"path": "/measurement-sets/IGVFDS7182DGDS/"
},
{
"category": "missing sequencing kit",
"detail": "Measurement set [IGVFDS7182DGDS](/measurement-sets/IGVFDS7182DGDS/) has sequence file(s) [IGVFFI2985VSGE](/sequence-files/IGVFFI2985VSGE/), [IGVFFI9039RXBK](/sequence-files/IGVFFI9039RXBK/), [IGVFFI5075LZIL](/sequence-files/IGVFFI5075LZIL/), [IGVFFI8727LXUE](/sequence-files/IGVFFI8727LXUE/), [IGVFFI2289LZDO](/sequence-files/IGVFFI2289LZDO/), [IGVFFI2814SFWJ](/sequence-files/IGVFFI2814SFWJ/), [IGVFFI3563HVLI](/sequence-files/IGVFFI3563HVLI/), [IGVFFI7757VRPV](/sequence-files/IGVFFI7757VRPV/) which lack specification of a `sequencing_kit`. Sequence files in a file set associated wtih bulk data should specify a sequencing kit.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_inconsistent_sequencing_kit",
"path": "/measurement-sets/IGVFDS7182DGDS/"
},
{
"category": "missing publication",
"detail": "Auxiliary set [IGVFDS9055BZJI](/auxiliary-sets/IGVFDS9055BZJI/) has no `publications`. Released and archived file sets are expected to be associated with a publication.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_file_set_missing_publication",
"path": "/auxiliary-sets/IGVFDS9055BZJI/"
},
{
"category": "missing publication",
"detail": "In vitro system [IGVFSM3056RYCY](/in-vitro-systems/IGVFSM3056RYCY/) has no `publications`. Released and archived samples are expected to be associated with a publication.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_sample_missing_publication",
"path": "/in-vitro-systems/IGVFSM3056RYCY/"
}
],
"WARNING": [
{
"category": "missing nucleic acid delivery",
"detail": "In vitro system [IGVFSM3056RYCY](/in-vitro-systems/IGVFSM3056RYCY/) has `construct_library_sets` but is missing `nucleic_acid_delivery`. Samples linked to construct library sets are expected to specify nucleic acid delivery method.",
"level": 40,
"level_name": "WARNING",
"name": "audit_missing_nucleic_acid_delivery",
"path": "/in-vitro-systems/IGVFSM3056RYCY/"
}
]
},
"auxiliary_sets": [
{
"@id": "/auxiliary-sets/IGVFDS9055BZJI/",
"accession": "IGVFDS9055BZJI",
"aliases": [
"jesse-engreitz:auxiliary_sortparams_CRUDO_MYC-Auxin6hrs-FF1"
],
"file_set_type": "cell sorting",
"status": "released"
}
],
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
"title": "Jesse Engreitz"
},
"title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
},
"construct_library_sets": [
{
"@id": "/construct-library-sets/IGVFDS7857SYCK/",
"accession": "IGVFDS7857SYCK",
"file_set_type": "guide library",
"integrated_content_files": [
{
"@id": "/tabular-files/IGVFFI3782KCTX/",
"accession": "IGVFFI3782KCTX",
"content_type": "guide RNA sequences",
"status": "released",
"summary": "guide RNA sequences"
}
],
"status": "released",
"summary": "guide (sgRNA) library targeting accessible genome regions in many genomic loci"
}
],
"control_for": [],
"creation_timestamp": "2024-05-21T17:34:55.266042+00:00",
"documents": [
"/documents/74d53b9c-ab1d-42e3-b679-d3a00ae3d842/"
],
"donors": [
{
"@id": "/human-donors/IGVFDO0384OZRW/",
"accession": "IGVFDO0384OZRW",
"aliases": [
"igvf:donor_of_HCT116"
],
"sex": "male",
"status": "released",
"taxa": "Homo sapiens"
}
],
"externally_hosted": false,
"file_set_type": "experimental data",
"files": [
{
"@id": "/configuration-files/IGVFFI9696GNVY/",
"accession": "IGVFFI9696GNVY",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR2_Rep2_S172"
],
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:50:05.733641+00:00",
"file_format": "yaml",
"file_size": 911,
"href": "/configuration-files/IGVFFI9696GNVY/@@download/IGVFFI9696GNVY.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/16b59842-5b12-4467-b04d-103cf5877cc6/IGVFFI9696GNVY.yaml.gz",
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.MYC-Auxin6hrs-FF1-BinA-PCR2_Rep2_S172.yaml",
"upload_status": "validation exempted"
},
{
"@id": "/configuration-files/IGVFFI9950ZGFX/",
"accession": "IGVFFI9950ZGFX",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR2_Rep1_S117"
],
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:49:50.556606+00:00",
"file_format": "yaml",
"file_size": 911,
"href": "/configuration-files/IGVFFI9950ZGFX/@@download/IGVFFI9950ZGFX.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/3cfd845a-c1f2-4494-8fec-d75ced5f1402/IGVFFI9950ZGFX.yaml.gz",
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.MYC-Auxin6hrs-FF1-BinA-PCR2_Rep1_S117.yaml",
"upload_status": "validation exempted"
},
{
"@id": "/configuration-files/IGVFFI9250ZNHJ/",
"accession": "IGVFFI9250ZNHJ",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR3_Rep2_S178"
],
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:50:35.297458+00:00",
"file_format": "yaml",
"file_size": 913,
"href": "/configuration-files/IGVFFI9250ZNHJ/@@download/IGVFFI9250ZNHJ.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/d780fed7-d3ce-4055-816a-5b755d5edbd7/IGVFFI9250ZNHJ.yaml.gz",
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.MYC-Auxin6hrs-FF1-BinA-PCR3_Rep2_S178.yaml",
"upload_status": "validation exempted"
},
{
"@id": "/configuration-files/IGVFFI8520ASYZ/",
"accession": "IGVFFI8520ASYZ",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR4_Rep1_S129"
],
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:50:49.625867+00:00",
"file_format": "yaml",
"file_size": 911,
"href": "/configuration-files/IGVFFI8520ASYZ/@@download/IGVFFI8520ASYZ.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/29ecfba9-0f25-4ffa-b1a1-26f36759d0e3/IGVFFI8520ASYZ.yaml.gz",
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.MYC-Auxin6hrs-FF1-BinA-PCR4_Rep1_S129.yaml",
"upload_status": "validation exempted"
},
{
"@id": "/configuration-files/IGVFFI5421EMLL/",
"accession": "IGVFFI5421EMLL",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR4_Rep2_S184"
],
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:51:06.039760+00:00",
"file_format": "yaml",
"file_size": 912,
"href": "/configuration-files/IGVFFI5421EMLL/@@download/IGVFFI5421EMLL.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/a45824f1-eaf9-4ea8-9a93-af99f63d677b/IGVFFI5421EMLL.yaml.gz",
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.MYC-Auxin6hrs-FF1-BinA-PCR4_Rep2_S184.yaml",
"upload_status": "validation exempted"
},
{
"@id": "/configuration-files/IGVFFI3540RMKW/",
"accession": "IGVFFI3540RMKW",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR3_Rep1_S123"
],
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:50:20.108502+00:00",
"file_format": "yaml",
"file_size": 914,
"href": "/configuration-files/IGVFFI3540RMKW/@@download/IGVFFI3540RMKW.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/2644eb3f-f700-4f26-9b31-3dc63808901e/IGVFFI3540RMKW.yaml.gz",
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.MYC-Auxin6hrs-FF1-BinA-PCR3_Rep1_S123.yaml",
"upload_status": "validation exempted"
},
{
"@id": "/sequence-files/IGVFFI2985VSGE/",
"accession": "IGVFFI2985VSGE",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR2_Rep1_S117"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-06-01T02:04:15.581021+00:00",
"file_format": "fastq",
"file_size": 16118182,
"href": "/sequence-files/IGVFFI2985VSGE/@@download/IGVFFI2985VSGE.fastq.gz",
"s3_uri": "s3://igvf-files/2024/06/01/a6b56fed-bb6c-499d-9084-39a73689bb26/IGVFFI2985VSGE.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008565/",
"status": "released",
"term_name": "Illumina HiSeq 2500"
},
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin6hrs-FF1-BinA-PCR2-Rep1_S117_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/configuration-files/IGVFFI4492TGXP/",
"accession": "IGVFFI4492TGXP",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR1_Rep1_S111"
],
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:49:20.902143+00:00",
"file_format": "yaml",
"file_size": 913,
"href": "/configuration-files/IGVFFI4492TGXP/@@download/IGVFFI4492TGXP.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/38c0812c-8a82-4c9c-9228-309afa49488f/IGVFFI4492TGXP.yaml.gz",
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.MYC-Auxin6hrs-FF1-BinA-PCR1_Rep1_S111.yaml",
"upload_status": "validation exempted"
},
{
"@id": "/sequence-files/IGVFFI9039RXBK/",
"accession": "IGVFFI9039RXBK",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR1_Rep2_S166"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-06-01T02:03:55.093307+00:00",
"file_format": "fastq",
"file_size": 25923298,
"href": "/sequence-files/IGVFFI9039RXBK/@@download/IGVFFI9039RXBK.fastq.gz",
"s3_uri": "s3://igvf-files/2024/06/01/5681d9d5-5614-49f6-8e1d-6b0d614ca272/IGVFFI9039RXBK.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008565/",
"status": "released",
"term_name": "Illumina HiSeq 2500"
},
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin6hrs-FF1-BinA-PCR1-Rep2_S166_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI5075LZIL/",
"accession": "IGVFFI5075LZIL",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR1_Rep1_S111"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-06-01T02:03:35.207316+00:00",
"file_format": "fastq",
"file_size": 15403805,
"href": "/sequence-files/IGVFFI5075LZIL/@@download/IGVFFI5075LZIL.fastq.gz",
"s3_uri": "s3://igvf-files/2024/06/01/03b3c416-bc75-4e50-8fd1-5fefa2fb4df7/IGVFFI5075LZIL.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008565/",
"status": "released",
"term_name": "Illumina HiSeq 2500"
},
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin6hrs-FF1-BinA-PCR1-Rep1_S111_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/configuration-files/IGVFFI0706VNBX/",
"accession": "IGVFFI0706VNBX",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR1_Rep2_S166"
],
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:49:35.867212+00:00",
"file_format": "yaml",
"file_size": 913,
"href": "/configuration-files/IGVFFI0706VNBX/@@download/IGVFFI0706VNBX.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/982d36a1-b66a-4d43-8c8f-1a989543e9e0/IGVFFI0706VNBX.yaml.gz",
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.MYC-Auxin6hrs-FF1-BinA-PCR1_Rep2_S166.yaml",
"upload_status": "validation exempted"
},
{
"@id": "/sequence-files/IGVFFI8727LXUE/",
"accession": "IGVFFI8727LXUE",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR3_Rep2_S178"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-06-01T02:05:15.834295+00:00",
"file_format": "fastq",
"file_size": 22446173,
"href": "/sequence-files/IGVFFI8727LXUE/@@download/IGVFFI8727LXUE.fastq.gz",
"s3_uri": "s3://igvf-files/2024/06/01/1dbb4680-b3a7-4df7-bc34-e0f832b7b518/IGVFFI8727LXUE.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008565/",
"status": "released",
"term_name": "Illumina HiSeq 2500"
},
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin6hrs-FF1-BinA-PCR3-Rep2_S178_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI2289LZDO/",
"accession": "IGVFFI2289LZDO",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR3_Rep1_S123"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-06-01T02:04:56.240190+00:00",
"file_format": "fastq",
"file_size": 22839579,
"href": "/sequence-files/IGVFFI2289LZDO/@@download/IGVFFI2289LZDO.fastq.gz",
"s3_uri": "s3://igvf-files/2024/06/01/dc46f206-0ff4-416a-943d-8a8fd2aab8a9/IGVFFI2289LZDO.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008565/",
"status": "released",
"term_name": "Illumina HiSeq 2500"
},
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin6hrs-FF1-BinA-PCR3-Rep1_S123_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI2814SFWJ/",
"accession": "IGVFFI2814SFWJ",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR4_Rep2_S184"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-06-01T02:05:53.312070+00:00",
"file_format": "fastq",
"file_size": 16750214,
"href": "/sequence-files/IGVFFI2814SFWJ/@@download/IGVFFI2814SFWJ.fastq.gz",
"s3_uri": "s3://igvf-files/2024/06/01/f07a5a48-6608-4996-a15f-0c7816ec5a9b/IGVFFI2814SFWJ.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008565/",
"status": "released",
"term_name": "Illumina HiSeq 2500"
},
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin6hrs-FF1-BinA-PCR4-Rep2_S184_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI3563HVLI/",
"accession": "IGVFFI3563HVLI",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR2_Rep2_S172"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-06-01T02:04:35.598955+00:00",
"file_format": "fastq",
"file_size": 15745670,
"href": "/sequence-files/IGVFFI3563HVLI/@@download/IGVFFI3563HVLI.fastq.gz",
"s3_uri": "s3://igvf-files/2024/06/01/37e8bbaf-bff1-4a6e-8cc3-810a4f5b6398/IGVFFI3563HVLI.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008565/",
"status": "released",
"term_name": "Illumina HiSeq 2500"
},
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin6hrs-FF1-BinA-PCR2-Rep2_S172_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI7757VRPV/",
"accession": "IGVFFI7757VRPV",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR4_Rep1_S129"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-06-01T02:05:34.600953+00:00",
"file_format": "fastq",
"file_size": 18966292,
"href": "/sequence-files/IGVFFI7757VRPV/@@download/IGVFFI7757VRPV.fastq.gz",
"s3_uri": "s3://igvf-files/2024/06/01/3315e005-e284-46a5-941b-3e1be1a8f03c/IGVFFI7757VRPV.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008565/",
"status": "released",
"term_name": "Illumina HiSeq 2500"
},
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin6hrs-FF1-BinA-PCR4-Rep1_S129_R1_001.fastq.gz",
"upload_status": "validated"
}
],
"input_for": [
"/analysis-sets/IGVFDS4544YEQM/"
],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"notes": "The readout 2e41e624-f6c7-4f0c-a07e-bc36cefdca39 was removed from this measurement set.",
"preferred_assay_title": "CRISPR FlowFISH screen",
"protocols": [
"https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1"
],
"release_timestamp": "2024-07-10T19:47:37.767701+00:00",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM3056RYCY/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM3056RYCY",
"aliases": [
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR1_Rep1_S111",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR1_Rep2_S166",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR2_Rep1_S117",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR2_Rep2_S172",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR3_Rep1_S123",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR3_Rep2_S178",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR4_Rep1_S129",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR4_Rep2_S184"
],
"classifications": [
"cell line"
],
"institutional_certificates": [],
"modifications": [
{
"@id": "/degron-modifications/95081d1b-08b4-49ed-b324-e53fc43d8456/",
"modality": "degradation",
"status": "released"
},
{
"@id": "/crispr-modifications/8543fa06-1720-4e7d-8e8f-7e24c1b1cf79/",
"modality": "interference",
"status": "released"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002824/",
"@type": [
"SampleTerm",
"OntologyTerm",
"Item"
],
"aliases": [
"igvf:HCT116"
],
"status": "released",
"summary": "HCT116",
"term_name": "HCT116"
}
],
"status": "released",
"summary": "Homo sapiens (male) HCT116 cell line (sorting details: FACS bin A for 0-10% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library",
"taxa": "Homo sapiens",
"treatments": [
{
"@id": "/treatments/d35868d1-c45b-49ae-8ff3-a9c0c22551c2/",
"purpose": "activation",
"status": "released",
"summary": "Treatment of 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours",
"treatment_type": "chemical"
}
]
}
],
"schema_version": "26",
"status": "released",
"submitted_by": {
"@id": "/users/3bd6f74c-8cf3-4c35-a94d-0c7c11654815/",
"title": "Chongyon Park"
},
"submitted_files_timestamp": "2024-06-01T02:03:35.207316+00:00",
"summary": "mixed CRISPR FlowFISH screen sorted on the expression of MYC integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci",
"targeted_genes": [
{
"@id": "/genes/ENSG00000136997/",
"geneid": "ENSG00000136997",
"name": "MYC proto-oncogene, bHLH transcription factor",
"status": "released",
"symbol": "MYC",
"synonyms": [
"bHLHe39",
"myc-related translation/localization regulatory factor",
"MYC proto-oncogene, bHLH transcription factor",
"MYC",
"c-Myc",
"v-myc avian myelocytomatosis viral oncogene homolog",
"proto-oncogene c-Myc",
"transcription factor p64",
"myc proto-oncogene protein",
"avian myelocytomatosis viral oncogene homolog",
"MRTL",
"class E basic helix-loop-helix protein 39",
"MYCC",
"v-myc myelocytomatosis viral oncogene homolog"
]
}
],
"uuid": "b8c80420-0ea3-4204-889e-b35885e9fb88"
}