IGVFDS7173YRIO

released

File Set Type

experimental data

Summary

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

CCND1

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Construct Library Sets

IGVFDS6351FGNG guide (sgRNA) library targeting accessible genome regions in many genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_CCND1_Auxin0hrs-FF1-BinB-PCR1-Rep1_S218

jesse-engreitz:measurement_CRUDO_CCND1_Auxin0hrs-FF1-BinB-PCR2-Rep1_S224

jesse-engreitz:measurement_CRUDO_CCND1_Auxin0hrs-FF1-BinB-PCR3-Rep1_S260

jesse-engreitz:measurement_CRUDO_CCND1_Auxin0hrs-FF1-BinB-PCR4-Rep1_S266

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM4779RXHT

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS3348ANJX

AuxiliarySet

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFFI2449YPRX, IGVFFI2197JQZH, IGVFFI7504AYGF, IGVFFI8736GAXG

jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin0hrs-FF1-BinB-PCR1-Rep1_S218
jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin0hrs-FF1-BinB-PCR2-Rep1_S224
jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin0hrs-FF1-BinB-PCR3-Rep1_S260
jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin0hrs-FF1-BinB-PCR4-Rep1_S266

Jesse Engreitz, Stanford

IGVFDS5464XLTY

AuxiliarySet

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

IGVFFI0860RTJY