IGVFDS7163VHFR

released
{
    "@context": "/terms/",
    "@id": "/measurement-sets/IGVFDS7163VHFR/",
    "@type": [
        "MeasurementSet",
        "FileSet",
        "Item"
    ],
    "accession": "IGVFDS7163VHFR",
    "aliases": [
        "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep2-FFrep4-BinD_AmpliconSequencing"
    ],
    "assay_term": {
        "@id": "/assay-terms/OBI_0003661/",
        "status": "released",
        "term_name": "in vitro CRISPR screen using flow cytometry"
    },
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "mismatched status",
                "detail": "Released in vitro system [IGVFSM8534PHPO](/in-vitro-systems/IGVFSM8534PHPO/) has in progress subobject construct library set [IGVFDS2385DHZO](/construct-library-sets/IGVFDS2385DHZO/).",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_item_status",
                "path": "/in-vitro-systems/IGVFSM8534PHPO/"
            },
            {
                "category": "missing sequencing kit",
                "detail": "Measurement set [IGVFDS7163VHFR](/measurement-sets/IGVFDS7163VHFR/) has sequence file(s) [IGVFFI2749KREX](/sequence-files/IGVFFI2749KREX/), [IGVFFI2460WNXJ](/sequence-files/IGVFFI2460WNXJ/), [IGVFFI3807TGBU](/sequence-files/IGVFFI3807TGBU/), [IGVFFI6807MGIS](/sequence-files/IGVFFI6807MGIS/), [IGVFFI7392YHPQ](/sequence-files/IGVFFI7392YHPQ/), [IGVFFI1780KKOS](/sequence-files/IGVFFI1780KKOS/), [IGVFFI4236FZZI](/sequence-files/IGVFFI4236FZZI/), [IGVFFI1200UTGE](/sequence-files/IGVFFI1200UTGE/), [IGVFFI8878EWVW](/sequence-files/IGVFFI8878EWVW/), [IGVFFI5638URJE](/sequence-files/IGVFFI5638URJE/), [IGVFFI0663MSMB](/sequence-files/IGVFFI0663MSMB/), [IGVFFI2074UPYM](/sequence-files/IGVFFI2074UPYM/), [IGVFFI5367AKQK](/sequence-files/IGVFFI5367AKQK/), [IGVFFI0931WHQH](/sequence-files/IGVFFI0931WHQH/), [IGVFFI8076ZQWY](/sequence-files/IGVFFI8076ZQWY/), [IGVFFI3293BVNE](/sequence-files/IGVFFI3293BVNE/), [IGVFFI7495CDOL](/sequence-files/IGVFFI7495CDOL/), [IGVFFI3329KGJC](/sequence-files/IGVFFI3329KGJC/), [IGVFFI5082NRLZ](/sequence-files/IGVFFI5082NRLZ/), [IGVFFI9875MXGG](/sequence-files/IGVFFI9875MXGG/), [IGVFFI7654ERMN](/sequence-files/IGVFFI7654ERMN/), [IGVFFI5274IPRI](/sequence-files/IGVFFI5274IPRI/), [IGVFFI1524NZVU](/sequence-files/IGVFFI1524NZVU/), [IGVFFI5539VTNZ](/sequence-files/IGVFFI5539VTNZ/), [IGVFFI0108HIDZ](/sequence-files/IGVFFI0108HIDZ/), [IGVFFI4483PGZB](/sequence-files/IGVFFI4483PGZB/), [IGVFFI8464HAXP](/sequence-files/IGVFFI8464HAXP/), [IGVFFI4594DJSZ](/sequence-files/IGVFFI4594DJSZ/), [IGVFFI2052JUGS](/sequence-files/IGVFFI2052JUGS/), [IGVFFI8900NOBX](/sequence-files/IGVFFI8900NOBX/), [IGVFFI0145AGBT](/sequence-files/IGVFFI0145AGBT/), [IGVFFI0742VHSS](/sequence-files/IGVFFI0742VHSS/), [IGVFFI4636SPYA](/sequence-files/IGVFFI4636SPYA/), [IGVFFI8292TRDJ](/sequence-files/IGVFFI8292TRDJ/), [IGVFFI4330GXJW](/sequence-files/IGVFFI4330GXJW/), [IGVFFI4702ZRCF](/sequence-files/IGVFFI4702ZRCF/), [IGVFFI5983UTXX](/sequence-files/IGVFFI5983UTXX/), [IGVFFI2202IWAB](/sequence-files/IGVFFI2202IWAB/), [IGVFFI6210ICND](/sequence-files/IGVFFI6210ICND/), [IGVFFI3045PEFU](/sequence-files/IGVFFI3045PEFU/), [IGVFFI9633YUAH](/sequence-files/IGVFFI9633YUAH/), [IGVFFI7742QOLR](/sequence-files/IGVFFI7742QOLR/), [IGVFFI4634CIEF](/sequence-files/IGVFFI4634CIEF/), [IGVFFI7881HDXU](/sequence-files/IGVFFI7881HDXU/), [IGVFFI8163LZNL](/sequence-files/IGVFFI8163LZNL/), [IGVFFI0749NNEU](/sequence-files/IGVFFI0749NNEU/), [IGVFFI8982OHCR](/sequence-files/IGVFFI8982OHCR/), [IGVFFI2373CBGF](/sequence-files/IGVFFI2373CBGF/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/measurement-sets/IGVFDS7163VHFR/"
            },
            {
                "category": "missing sequence specification",
                "detail": "Measurement set [IGVFDS7163VHFR](/measurement-sets/IGVFDS7163VHFR/) has sequence file(s): [IGVFFI2749KREX](/sequence-files/IGVFFI2749KREX/), [IGVFFI2460WNXJ](/sequence-files/IGVFFI2460WNXJ/), [IGVFFI3807TGBU](/sequence-files/IGVFFI3807TGBU/), [IGVFFI6807MGIS](/sequence-files/IGVFFI6807MGIS/), [IGVFFI7392YHPQ](/sequence-files/IGVFFI7392YHPQ/), [IGVFFI1780KKOS](/sequence-files/IGVFFI1780KKOS/), [IGVFFI4236FZZI](/sequence-files/IGVFFI4236FZZI/), [IGVFFI1200UTGE](/sequence-files/IGVFFI1200UTGE/), [IGVFFI8878EWVW](/sequence-files/IGVFFI8878EWVW/), [IGVFFI5638URJE](/sequence-files/IGVFFI5638URJE/), [IGVFFI0663MSMB](/sequence-files/IGVFFI0663MSMB/), [IGVFFI2074UPYM](/sequence-files/IGVFFI2074UPYM/), [IGVFFI5367AKQK](/sequence-files/IGVFFI5367AKQK/), [IGVFFI0931WHQH](/sequence-files/IGVFFI0931WHQH/), [IGVFFI8076ZQWY](/sequence-files/IGVFFI8076ZQWY/), [IGVFFI3293BVNE](/sequence-files/IGVFFI3293BVNE/), [IGVFFI7495CDOL](/sequence-files/IGVFFI7495CDOL/), [IGVFFI3329KGJC](/sequence-files/IGVFFI3329KGJC/), [IGVFFI5082NRLZ](/sequence-files/IGVFFI5082NRLZ/), [IGVFFI9875MXGG](/sequence-files/IGVFFI9875MXGG/), [IGVFFI7654ERMN](/sequence-files/IGVFFI7654ERMN/), [IGVFFI5274IPRI](/sequence-files/IGVFFI5274IPRI/), [IGVFFI1524NZVU](/sequence-files/IGVFFI1524NZVU/), [IGVFFI5539VTNZ](/sequence-files/IGVFFI5539VTNZ/), [IGVFFI0108HIDZ](/sequence-files/IGVFFI0108HIDZ/), [IGVFFI4483PGZB](/sequence-files/IGVFFI4483PGZB/), [IGVFFI8464HAXP](/sequence-files/IGVFFI8464HAXP/), [IGVFFI4594DJSZ](/sequence-files/IGVFFI4594DJSZ/), [IGVFFI2052JUGS](/sequence-files/IGVFFI2052JUGS/), [IGVFFI8900NOBX](/sequence-files/IGVFFI8900NOBX/), [IGVFFI0145AGBT](/sequence-files/IGVFFI0145AGBT/), [IGVFFI0742VHSS](/sequence-files/IGVFFI0742VHSS/), [IGVFFI4636SPYA](/sequence-files/IGVFFI4636SPYA/), [IGVFFI8292TRDJ](/sequence-files/IGVFFI8292TRDJ/), [IGVFFI4330GXJW](/sequence-files/IGVFFI4330GXJW/), [IGVFFI4702ZRCF](/sequence-files/IGVFFI4702ZRCF/), [IGVFFI5983UTXX](/sequence-files/IGVFFI5983UTXX/), [IGVFFI2202IWAB](/sequence-files/IGVFFI2202IWAB/), [IGVFFI6210ICND](/sequence-files/IGVFFI6210ICND/), [IGVFFI3045PEFU](/sequence-files/IGVFFI3045PEFU/), [IGVFFI9633YUAH](/sequence-files/IGVFFI9633YUAH/), [IGVFFI7742QOLR](/sequence-files/IGVFFI7742QOLR/), [IGVFFI4634CIEF](/sequence-files/IGVFFI4634CIEF/), [IGVFFI7881HDXU](/sequence-files/IGVFFI7881HDXU/), [IGVFFI8163LZNL](/sequence-files/IGVFFI8163LZNL/), [IGVFFI0749NNEU](/sequence-files/IGVFFI0749NNEU/), [IGVFFI8982OHCR](/sequence-files/IGVFFI8982OHCR/), [IGVFFI2373CBGF](/sequence-files/IGVFFI2373CBGF/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_missing_seqspec",
                "path": "/measurement-sets/IGVFDS7163VHFR/"
            }
        ],
        "WARNING": [
            {
                "category": "missing auxiliary set",
                "detail": "Measurement set [IGVFDS7163VHFR](/measurement-sets/IGVFDS7163VHFR/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
                "level": 40,
                "level_name": "WARNING",
                "name": "audit_missing_auxiliary_set",
                "path": "/measurement-sets/IGVFDS7163VHFR/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization",
        "contact_pi": {
            "@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
            "title": "Jesse Engreitz"
        },
        "title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
    },
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            "@id": "/measurement-sets/IGVFDS4869FXYY/",
            "accession": "IGVFDS4869FXYY",
            "aliases": [
                "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-Jurkat-PE2_Exp2_NegControl_AmpliconSequencing"
            ],
            "status": "released"
        }
    ],
    "control_for": [],
    "creation_timestamp": "2023-12-13T08:48:15.791376+00:00",
    "donors": [
        {
            "@id": "/human-donors/IGVFDO1080XFGV/",
            "accession": "IGVFDO1080XFGV",
            "aliases": [
                "igvf:donor_of_Jurkat"
            ],
            "sex": "male",
            "status": "released",
            "taxa": "Homo sapiens"
        }
    ],
    "externally_hosted": false,
    "file_set_type": "experimental data",
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        },
        {
            "@id": "/sequence-files/IGVFFI0663MSMB/",
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            "s3_uri": "s3://igvf-files/2023/12/13/858404b6-d06d-4582-a2fe-3faae1d263da/IGVFFI2373CBGF.fastq.gz",
            "sequencing_platform": {
                "@id": "/platform-terms/EFO_0008566/",
                "status": "released",
                "term_name": "Illumina NextSeq 550"
            },
            "status": "released",
            "submitted_file_name": "230423pPPIFEnformerJurkat-Jurkat-PE-pPPIF_Enformer-Amp-pPPIF-BioRep2-FFRep4_BinD-PCRrep8_S380_R1_001.fastq.gz",
            "upload_status": "validated"
        }
    ],
    "input_for": [
        "/analysis-sets/IGVFDS6299IJHG/"
    ],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "notes": "The readout 2e41e624-f6c7-4f0c-a07e-bc36cefdca39 was removed from this measurement set. This measurement set previously used Variant FlowFISH as a preferred_assay_title, but this enum is now removed. So it has been defaulted to Variant-EFFECTS.",
    "preferred_assay_title": "Variant-EFFECTS",
    "protocols": [
        "https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
    ],
    "release_timestamp": "2023-12-20T23:08:31.765727+00:00",
    "samples": [
        {
            "@id": "/in-vitro-systems/IGVFSM8534PHPO/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM8534PHPO",
            "aliases": [
                "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep2-FFrep4-BinD"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                {
                    "@id": "/construct-library-sets/IGVFDS2385DHZO/",
                    "accession": "IGVFDS2385DHZO",
                    "file_set_type": "guide library",
                    "status": "in progress",
                    "summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
                }
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0002796/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:Jurkat"
                    ],
                    "status": "released",
                    "summary": "Jurkat",
                    "term_name": "Jurkat"
                }
            ],
            "status": "released",
            "summary": "Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        }
    ],
    "schema_version": "23",
    "status": "released",
    "submitted_by": {
        "@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
        "title": "Michael Montgomery"
    },
    "submitted_files_timestamp": "2023-12-13T10:24:58.816683+00:00",
    "summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
    "targeted_genes": [
        {
            "@id": "/genes/ENSG00000108179/",
            "geneid": "ENSG00000108179",
            "name": "peptidylprolyl isomerase F",
            "status": "released",
            "symbol": "PPIF",
            "synonyms": [
                "rotamase F",
                "CYP3",
                "cyclophilin F",
                "mitochondrial cyclophilin",
                "peptidyl-prolyl cis-trans isomerase, mitochondrial",
                "CypD",
                "Cyp-D",
                "cyclophilin 3",
                "cyclophilin D",
                "CyP-M",
                "peptidyl-prolyl cis-trans isomerase F, mitochondrial",
                "PPIase F"
            ]
        }
    ],
    "uuid": "db72b3c4-daf7-4c01-ae5a-27a4a79c197f"
}