IGVFDS7147ANAC

released

File Set Type

experimental data

Summary

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

KITLG

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Construct Library Sets

IGVFDS7846UISG guide (sgRNA) library targeting accessible genome regions in many genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF2-BinB-PCR1_S14

jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF2-BinB-PCR2_S20

jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF2-BinB-PCR3_S110

jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF2-BinB-PCR4_S116

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM3263KXLP

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS1409CHZY

AuxiliarySet

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFFI6322UNED, IGVFFI2295FIDE, IGVFFI1480XPQY, IGVFFI3742XLEW

jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF2-BinB-PCR1_S14
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF2-BinB-PCR2_S20
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF2-BinB-PCR3_S110
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF2-BinB-PCR4_S116

Jesse Engreitz, Stanford

IGVFDS4522DABT

AuxiliarySet

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

IGVFFI5182VLZN