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MeasurementSet
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IGVFDS7065TFMP
IGVFDS7065TFMP
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This object is in preview status. Metadata is subject to change until this object is released.
File Set Type
experimental data
Summary
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci
Assay Term
in vitro CRISPR screen using single-cell RNA-seq
Sample Summaries
Homo sapiens (female) TeloHAEC cell line modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
Protocols
https://www.protocols.io/view/systematic-perturb-seq-to-discover-endothelial-cel-261ged73yv47/v1
Aliases
jesse-engreitz:measurement_set-scRNAseq_2kG_11AMDox_3
Publications
Convergence of coronary artery disease genes onto endothelial cell programs
Attribution
Jesse Engreitz, Stanford
(
HG011972
—
Jesse Engreitz
)
Sequencing Results (Illumina)
15 items
1
2
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Read Names
Associated seqspec Files
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI0251WYUD
fastq
reads
R1
3
Illumina NovaSeq 6000
1.4 GB
Jesse Engreitz, Stanford
validated
IGVFFI0698WEDS
fastq
reads
I1
1
Illumina NovaSeq 6000
212 MB
Jesse Engreitz, Stanford
validated
IGVFFI0823TQBB
fastq
reads
I1
5
Illumina NovaSeq 6000
2.4 GB
Jesse Engreitz, Stanford
validated
IGVFFI1019PRQV
fastq
reads
R1
1
Illumina NovaSeq 6000
657 MB
Jesse Engreitz, Stanford
validated
IGVFFI1655PXYJ
fastq
reads
I1
3
Illumina NovaSeq 6000
421 MB
Jesse Engreitz, Stanford
validated
IGVFFI2941VXJV
fastq
reads
I1
4
Illumina NovaSeq 6000
543 MB
Jesse Engreitz, Stanford
validated
IGVFFI5087LXYZ
fastq
reads
R1
2
Illumina NovaSeq 6000
1.5 GB
Jesse Engreitz, Stanford
validated
IGVFFI5551REWL
fastq
reads
R2
1
Illumina NovaSeq 6000
915 MB
Jesse Engreitz, Stanford
validated
IGVFFI5728PPFM
fastq
reads
I1
2
Illumina NovaSeq 6000
424 MB
Jesse Engreitz, Stanford
validated
IGVFFI6437DQSS
fastq
reads
R2
5
Illumina NovaSeq 6000
12 GB
Jesse Engreitz, Stanford
validated
Samples
1 item
Accession
Type
Sample Terms
Associated Construct Library Sets
Summary
IGVFSM6249JKCM
InVitroSystem
TeloHAEC
IGVFDS1298TUYG
Homo sapiens (female) TeloHAEC cell line modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO3459DFTW
igvf:donor_of_teloHAEC
female
Homo sapiens
Associated Construct Library Sets
1 item
Accession
Summary
Library Design Files
IGVFDS1298TUYG
guide (sgRNA) library targeting genes in many genomic loci
IGVFFI9754AGFB
File Sets Using This Measurement Set as an Input
1 item
Accession
Type
Summary
Aliases
Lab
IGVFDS7943IIWZ
AnalysisSet
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci
jesse-engreitz:analysis_set_TeloHAEC_Perturb-seq_2kG
Jesse Engreitz, Stanford
Auxiliary Datasets
1 item
Accession
Type
Summary
Files
Aliases
Lab
IGVFDS8448SPRJ
AuxiliarySet
gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci
IGVFFI2577DAKQ
,
IGVFFI2560BMOQ
,
IGVFFI2599AGWT
,
IGVFFI7121UVOD
jesse-engreitz:auxiliary_sets-scRNAseq_2kG_11AMDox_3
Jesse Engreitz, Stanford