IGVFDS6992SRVM

released

File Set Type

experimental data

Summary

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

FAM3C

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Construct Library Sets

IGVFDS1891GTLU guide (sgRNA) library targeting accessible genome regions in many genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_FAM3C_Auxin6hrs-FF1-BinC-PCR1-Rep1_S135

jesse-engreitz:measurement_CRUDO_FAM3C_Auxin6hrs-FF1-BinC-PCR2-Rep1_S141

jesse-engreitz:measurement_CRUDO_FAM3C_Auxin6hrs-FF1-BinC-PCR3-Rep1_S189

jesse-engreitz:measurement_CRUDO_FAM3C_Auxin6hrs-FF1-BinC-PCR4-Rep1_S195

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM5752WTYA

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS7308SSJS

AuxiliarySet

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFFI6247BYIS, IGVFFI3369KHSA, IGVFFI1726SABC, IGVFFI6913STDU

jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin6hrs-FF1-BinC-PCR1-Rep1_S135
jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin6hrs-FF1-BinC-PCR2-Rep1_S141
jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin6hrs-FF1-BinC-PCR3-Rep1_S189
jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin6hrs-FF1-BinC-PCR4-Rep1_S195

Jesse Engreitz, Stanford

IGVFDS9190KTAF

AuxiliarySet

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

IGVFFI8850HGLT