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IGVFDS6874YIXA

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File Set Type
experimental data
Summary
prime editing Variant-EFFECTS
Assay Term
in vitro CRISPR screen using flow cytometry
Targeted Genes
Sample Summaries
  • Jurkat cell line, male, Homo sapiens treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)
Protocols
Aliases
jesse-engreitz:Jurkat_Stim-IL2RA_prom-NegControl_AmpliconSequencing

Sequencing Results (Illumina)

16 items
fastq
reads
R2
1
TCCTTGGT:CCTG
7.5 KB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
CGTTACCA:CCTG
6.7 KB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
AACGCATT:CCTG
4.6 KB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
AACGCATT:CCTG
2.2 KB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
CGTTACCA:CCTG
6.8 KB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
AACGCATT:CCTG
2.3 KB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
ACAGGTAT:CCTG
2.9 KB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
ACAGGTAT:CCTG
2.8 KB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
ACAGGTAT:CCTG
6.6 KB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
TCCTTGGT:CCTG
3.4 KB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Jurkat cell line, male, Homo sapiens treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_Jurkat
male
Homo sapiens

File Sets Using This Measurement Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
prime editing Variant-EFFECTS targeting IL2RA: variant effects
  • jesse-engreitz:IL2RA_promoter_tiling_Jurkat-Stimulation_Variant-EFFECTS_analysis
Jesse Engreitz, Stanford

File Sets Controlled by This Measurement Set

24 items
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep2-FFRep3-BinC_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep1-BinD_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep2-BinD_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep3-BinD_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep2-BinB_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep1-BinB_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep2-FFRep2-BinC_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep2-FFRep3-BinB_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep3-BinB_AmpliconSequencing
Jesse Engreitz, Stanford
MeasurementSet
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep2-FFRep2-BinD_AmpliconSequencing
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford