Homo sapiens (male) Jurkat cell line treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)
Homo sapiens (male) Jurkat cell line treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)
CRISPR prime editing Variant-EFFECTS sorted on the expression of IL2RA integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
CRISPR prime editing Variant-EFFECTS sorted on the expression of IL2RA integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
CRISPR prime editing Variant-EFFECTS sorted on the expression of IL2RA integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
CRISPR prime editing Variant-EFFECTS sorted on the expression of IL2RA integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
CRISPR prime editing Variant-EFFECTS sorted on the expression of IL2RA integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
CRISPR prime editing Variant-EFFECTS sorted on the expression of IL2RA integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
CRISPR prime editing Variant-EFFECTS sorted on the expression of IL2RA integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
CRISPR prime editing Variant-EFFECTS sorted on the expression of IL2RA integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
CRISPR prime editing Variant-EFFECTS sorted on the expression of IL2RA integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
CRISPR prime editing Variant-EFFECTS sorted on the expression of IL2RA integrating a guide (sgRNA) library targeting sequence variants in a genomic locus