{
"@context": "/terms/",
"@id": "/measurement-sets/IGVFDS6865SDZA/",
"@type": [
"MeasurementSet",
"FileSet",
"Item"
],
"accession": "IGVFDS6865SDZA",
"aliases": [
"jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF1-BinC-PCR1-Rep1_S243",
"jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF1-BinC-PCR2-Rep1_S249",
"jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF1-BinC-PCR3-Rep1_S285",
"jesse-engreitz:measurement_CRUDO_CCND1_Auxin6hrs-FF1-BinC-PCR4-Rep1_S291"
],
"assay_term": {
"@id": "/assay-terms/OBI_0003661/",
"assay_slims": [
"CRISPR screens"
],
"status": "released",
"term_name": "in vitro CRISPR screen using flow cytometry"
},
"audit": {
"INTERNAL_ACTION": [
{
"category": "missing publication",
"detail": "Measurement set [IGVFDS6865SDZA](/measurement-sets/IGVFDS6865SDZA/) has no `publications`. Released and archived file sets are expected to be associated with a publication.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_file_set_missing_publication",
"path": "/measurement-sets/IGVFDS6865SDZA/"
},
{
"category": "missing sequencing kit",
"detail": "Measurement set [IGVFDS6865SDZA](/measurement-sets/IGVFDS6865SDZA/) has sequence file(s) [IGVFFI4815RJIT](/sequence-files/IGVFFI4815RJIT/), [IGVFFI9181YPZG](/sequence-files/IGVFFI9181YPZG/), [IGVFFI5154USHE](/sequence-files/IGVFFI5154USHE/), [IGVFFI1822HTFR](/sequence-files/IGVFFI1822HTFR/) which lack specification of a `sequencing_kit`. Sequence files in a file set associated wtih bulk data should specify a sequencing kit.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_inconsistent_sequencing_kit",
"path": "/measurement-sets/IGVFDS6865SDZA/"
},
{
"category": "missing publication",
"detail": "In vitro system [IGVFSM2025NTWT](/in-vitro-systems/IGVFSM2025NTWT/) has no `publications`. Released and archived samples are expected to be associated with a publication.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_sample_missing_publication",
"path": "/in-vitro-systems/IGVFSM2025NTWT/"
},
{
"category": "missing publication",
"detail": "Auxiliary set [IGVFDS6842FAIJ](/auxiliary-sets/IGVFDS6842FAIJ/) has no `publications`. Released and archived file sets are expected to be associated with a publication.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_file_set_missing_publication",
"path": "/auxiliary-sets/IGVFDS6842FAIJ/"
}
],
"WARNING": [
{
"category": "missing nucleic acid delivery",
"detail": "In vitro system [IGVFSM2025NTWT](/in-vitro-systems/IGVFSM2025NTWT/) has `construct_library_sets` but is missing `nucleic_acid_delivery`. Samples linked to construct library sets are expected to specify nucleic acid delivery method.",
"level": 40,
"level_name": "WARNING",
"name": "audit_missing_nucleic_acid_delivery",
"path": "/in-vitro-systems/IGVFSM2025NTWT/"
}
]
},
"auxiliary_sets": [
{
"@id": "/auxiliary-sets/IGVFDS6842FAIJ/",
"accession": "IGVFDS6842FAIJ",
"aliases": [
"jesse-engreitz:auxiliary_sortparams_CRUDO_CCND1_Auxin6hrs-FF1"
],
"file_set_type": "cell sorting",
"status": "released"
}
],
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
"title": "Jesse Engreitz"
},
"title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
},
"construct_library_sets": [
{
"@id": "/construct-library-sets/IGVFDS6351FGNG/",
"accession": "IGVFDS6351FGNG",
"file_set_type": "guide library",
"integrated_content_files": [
{
"@id": "/tabular-files/IGVFFI5924HOIN/",
"accession": "IGVFFI5924HOIN",
"content_type": "guide RNA sequences",
"status": "released",
"summary": "guide RNA sequences"
}
],
"status": "released",
"summary": "guide (sgRNA) library targeting accessible genome regions in many genomic loci"
}
],
"control_for": [],
"creation_timestamp": "2024-05-13T22:16:37.385966+00:00",
"documents": [
"/documents/74d53b9c-ab1d-42e3-b679-d3a00ae3d842/"
],
"donors": [
{
"@id": "/human-donors/IGVFDO0384OZRW/",
"accession": "IGVFDO0384OZRW",
"aliases": [
"igvf:donor_of_HCT116"
],
"sex": "male",
"status": "released",
"taxa": "Homo sapiens"
}
],
"externally_hosted": false,
"file_set_type": "experimental data",
"files": [
{
"@id": "/configuration-files/IGVFFI5770SSOD/",
"accession": "IGVFFI5770SSOD",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_CCND1_Auxin6hrs-FF1-BinC-PCR2-Rep1_S249"
],
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:30:11.057070+00:00",
"file_format": "yaml",
"file_size": 913,
"href": "/configuration-files/IGVFFI5770SSOD/@@download/IGVFFI5770SSOD.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/f04bd725-7b38-4c75-9e45-1041c1287673/IGVFFI5770SSOD.yaml.gz",
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.CCND1_Auxin6hrs-FF1-BinC-PCR2-Rep1_S249.yaml",
"upload_status": "validation exempted"
},
{
"@id": "/configuration-files/IGVFFI6283ZAYL/",
"accession": "IGVFFI6283ZAYL",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_CCND1_Auxin6hrs-FF1-BinC-PCR4-Rep1_S291"
],
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:30:39.608599+00:00",
"file_format": "yaml",
"file_size": 915,
"href": "/configuration-files/IGVFFI6283ZAYL/@@download/IGVFFI6283ZAYL.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/4b331ff2-0360-49a1-b4d0-ea10586e2b22/IGVFFI6283ZAYL.yaml.gz",
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.CCND1_Auxin6hrs-FF1-BinC-PCR4-Rep1_S291.yaml",
"upload_status": "validation exempted"
},
{
"@id": "/configuration-files/IGVFFI7447YRKM/",
"accession": "IGVFFI7447YRKM",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_CCND1_Auxin6hrs-FF1-BinC-PCR3-Rep1_S285"
],
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:30:25.190772+00:00",
"file_format": "yaml",
"file_size": 914,
"href": "/configuration-files/IGVFFI7447YRKM/@@download/IGVFFI7447YRKM.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/cdfc9713-a646-4d6f-b627-9aaaf1374519/IGVFFI7447YRKM.yaml.gz",
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.CCND1_Auxin6hrs-FF1-BinC-PCR3-Rep1_S285.yaml",
"upload_status": "validation exempted"
},
{
"@id": "/configuration-files/IGVFFI2287BUST/",
"accession": "IGVFFI2287BUST",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_CCND1_Auxin6hrs-FF1-BinC-PCR1-Rep1_S243"
],
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:29:56.774806+00:00",
"file_format": "yaml",
"file_size": 913,
"href": "/configuration-files/IGVFFI2287BUST/@@download/IGVFFI2287BUST.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/e6b0b27a-30ec-450a-bdc9-fb75a14aa630/IGVFFI2287BUST.yaml.gz",
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.CCND1_Auxin6hrs-FF1-BinC-PCR1-Rep1_S243.yaml",
"upload_status": "validation exempted"
},
{
"@id": "/sequence-files/IGVFFI4815RJIT/",
"accession": "IGVFFI4815RJIT",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_CCND1_Auxin6hrs-FF1-BinC-PCR2-Rep1_S249"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-06-01T01:42:24.307093+00:00",
"file_format": "fastq",
"file_size": 13692997,
"href": "/sequence-files/IGVFFI4815RJIT/@@download/IGVFFI4815RJIT.fastq.gz",
"s3_uri": "s3://igvf-files/2024/06/01/92d90ecc-e4ed-4599-b198-f2e193df4f18/IGVFFI4815RJIT.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008565/",
"status": "released",
"term_name": "Illumina HiSeq 2500"
},
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/CCND1-Auxin6hrs-FF1-BinC-PCR2-Rep1_S249_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI9181YPZG/",
"accession": "IGVFFI9181YPZG",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_CCND1_Auxin6hrs-FF1-BinC-PCR4-Rep1_S291"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-06-01T01:42:59.603037+00:00",
"file_format": "fastq",
"file_size": 29718656,
"href": "/sequence-files/IGVFFI9181YPZG/@@download/IGVFFI9181YPZG.fastq.gz",
"s3_uri": "s3://igvf-files/2024/06/01/508139dd-33f5-408e-ad6e-0a64c0e2e5b0/IGVFFI9181YPZG.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008565/",
"status": "released",
"term_name": "Illumina HiSeq 2500"
},
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/CCND1-Auxin6hrs-FF1-BinC-PCR4-Rep1_S291_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI5154USHE/",
"accession": "IGVFFI5154USHE",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_CCND1_Auxin6hrs-FF1-BinC-PCR3-Rep1_S285"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-06-01T01:42:40.824548+00:00",
"file_format": "fastq",
"file_size": 25660281,
"href": "/sequence-files/IGVFFI5154USHE/@@download/IGVFFI5154USHE.fastq.gz",
"s3_uri": "s3://igvf-files/2024/06/01/ac96ddec-539f-4aae-b14a-36bbc1b59a5a/IGVFFI5154USHE.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008565/",
"status": "released",
"term_name": "Illumina HiSeq 2500"
},
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/CCND1-Auxin6hrs-FF1-BinC-PCR3-Rep1_S285_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI1822HTFR/",
"accession": "IGVFFI1822HTFR",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_CCND1_Auxin6hrs-FF1-BinC-PCR1-Rep1_S243"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-06-01T01:42:06.604856+00:00",
"file_format": "fastq",
"file_size": 14278365,
"href": "/sequence-files/IGVFFI1822HTFR/@@download/IGVFFI1822HTFR.fastq.gz",
"s3_uri": "s3://igvf-files/2024/06/01/f3127739-e58d-4a5a-9c33-8e7d7558119e/IGVFFI1822HTFR.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008565/",
"status": "released",
"term_name": "Illumina HiSeq 2500"
},
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/CCND1-Auxin6hrs-FF1-BinC-PCR1-Rep1_S243_R1_001.fastq.gz",
"upload_status": "validated"
}
],
"input_for": [
"/analysis-sets/IGVFDS1569RNPD/"
],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"notes": "The readout 2e41e624-f6c7-4f0c-a07e-bc36cefdca39 was removed from this measurement set.",
"preferred_assay_title": "CRISPR FlowFISH screen",
"protocols": [
"https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1"
],
"release_timestamp": "2024-07-10T19:39:13.605231+00:00",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM2025NTWT/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM2025NTWT",
"aliases": [
"jesse-engreitz:in-vitro-system_CRUDO_CCND1_Auxin6hrs-FF1-BinC-PCR1-Rep1_S243",
"jesse-engreitz:in-vitro-system_CRUDO_CCND1_Auxin6hrs-FF1-BinC-PCR2-Rep1_S249",
"jesse-engreitz:in-vitro-system_CRUDO_CCND1_Auxin6hrs-FF1-BinC-PCR3-Rep1_S285",
"jesse-engreitz:in-vitro-system_CRUDO_CCND1_Auxin6hrs-FF1-BinC-PCR4-Rep1_S291"
],
"classifications": [
"cell line"
],
"institutional_certificates": [],
"modifications": [
{
"@id": "/degron-modifications/95081d1b-08b4-49ed-b324-e53fc43d8456/",
"modality": "degradation",
"status": "released"
},
{
"@id": "/crispr-modifications/8543fa06-1720-4e7d-8e8f-7e24c1b1cf79/",
"modality": "interference",
"status": "released"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002824/",
"@type": [
"SampleTerm",
"OntologyTerm",
"Item"
],
"aliases": [
"igvf:HCT116"
],
"status": "released",
"summary": "HCT116",
"term_name": "HCT116"
}
],
"status": "released",
"summary": "Homo sapiens (male) HCT116 cell line (sorting details: FACS bin C for 35-45% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library",
"taxa": "Homo sapiens",
"treatments": [
{
"@id": "/treatments/d35868d1-c45b-49ae-8ff3-a9c0c22551c2/",
"purpose": "activation",
"status": "released",
"summary": "Treatment of 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours",
"treatment_type": "chemical"
}
]
}
],
"schema_version": "26",
"status": "released",
"submitted_by": {
"@id": "/users/3bd6f74c-8cf3-4c35-a94d-0c7c11654815/",
"title": "Chongyon Park"
},
"submitted_files_timestamp": "2024-06-01T01:42:06.604856+00:00",
"summary": "mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci",
"targeted_genes": [
{
"@id": "/genes/ENSG00000110092/",
"geneid": "ENSG00000110092",
"name": "cyclin D1",
"status": "released",
"symbol": "CCND1",
"synonyms": [
"U21B31",
"cyclin D1",
"B-cell CLL/lymphoma 1",
"PRAD1 oncogene",
"PRAD1",
"CCND1",
"G1/S-specific cyclin-D1",
"BCL-1 oncogene",
"BCL1",
"D11S287E",
"B-cell lymphoma 1 protein"
]
}
],
"uuid": "c41aeae3-fcfa-4697-b87c-5fec5f42e1a2"
}