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IGVFDS6859ZHTQ

released
File Set Type
experimental data
Summary
cas mediated mutagenesis (Variant FlowFISH) integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Assay Term
cas mediated mutagenesis
Sample Summaries
  • THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 0-10% expression of PPIF) modified with a guide library
Construct Library Sets
  • IGVFDS6377KNCH Guide (sgRNA) library targeting sequence variants in a genomic locus
Protocols
Aliases
jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep1-FFrep2-BinA_AmpliconSequencing

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 0-10% expression of PPIF) modified with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_THP1
male
Homo sapiens

Sequencing Results (Illumina)

8 items
fastq
reads
I1
1
Illumina NextSeq 550
ACTAAGAC:TGAC
15 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
Illumina NextSeq 550
ACAGGTAT:TGAC
78 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
Illumina NextSeq 550
ACTAAGAC:TGAC
41 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
Illumina NextSeq 550
ACTAAGAC:TGAC
74 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
Illumina NextSeq 550
ACAGGTAT:TGAC
16 MB
Jesse Engreitz, Stanford
validated
fastq
reads
R1
1
Illumina NextSeq 550
ACAGGTAT:TGAC
43 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
Illumina NextSeq 550
ACAGGTAT:TGAC
15 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I2
1
Illumina NextSeq 550
ACTAAGAC:TGAC
14 MB
Jesse Engreitz, Stanford
validated

Control File Sets

1 item
Accession
Summary
Aliases
Lab
cas mediated mutagenesis (Variant FlowFISH)
  • jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-THP1-PE2_Exp3_NegControl_AmpliconSequencing
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford