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INDEXED
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MeasurementSet
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IGVFDS6764PIJI
IGVFDS6764PIJI
released
Json view
File Set Type
experimental data
Summary
CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
Assay Term
in vitro CRISPR screen using single-cell RNA-seq
Sample Summaries
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
Construct Library Sets
IGVFDS0821YMMV
guide (sgRNA) library targeting transcription start sites in 24 genes
Protocols
https://www.protocols.io/view/perturb-seq-characterizing-regulators-of-t-cell-fu-c5s9y6h6
https://www.protocols.io/view/crispra-tiling-screens-c5rfy53n
Aliases
charles-gersbach:cd8-tcells-perturb-seq-crispra-gex-rep1
Publications
Transcriptional and epigenetic regulators of human CD8+ T cell function identified through orthogonal CRISPR screens
Sequencing Results (Illumina)
8 items
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Associated seqspec Files
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI1893OEIP
fastq
reads
R1
1
IGVFFI4202MHFC
Illumina NovaSeq 6000
2
8.3 GB
Charles Gersbach, Duke
validated
IGVFFI2514FKSS
fastq
reads
R1
1
IGVFFI3921CPXY
Illumina NovaSeq 6000
4
8.4 GB
Charles Gersbach, Duke
validated
IGVFFI3522PYTD
fastq
reads
R2
1
IGVFFI4202MHFC
Illumina NovaSeq 6000
2
21 GB
Charles Gersbach, Duke
validated
IGVFFI5126PDVA
fastq
reads
R2
1
IGVFFI3921CPXY
Illumina NovaSeq 6000
4
21 GB
Charles Gersbach, Duke
validated
IGVFFI6949VPVV
fastq
reads
R2
1
IGVFFI2121DSKN
Illumina NovaSeq 6000
1
21 GB
Charles Gersbach, Duke
validated
IGVFFI7715PXEN
fastq
reads
R1
1
IGVFFI4327JRXJ
Illumina NovaSeq 6000
3
8.4 GB
Charles Gersbach, Duke
validated
IGVFFI7791HIRG
fastq
reads
R1
1
IGVFFI2121DSKN
Illumina NovaSeq 6000
1
8.4 GB
Charles Gersbach, Duke
validated
IGVFFI9683XENS
fastq
reads
R2
1
IGVFFI4327JRXJ
Illumina NovaSeq 6000
3
21 GB
Charles Gersbach, Duke
validated
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM8036VPRU
PrimaryCell
CD8-positive, alpha-beta memory T cell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO7605NZFO
charles-gersbach:cd8-tcells-donor-6
unspecified
Homo sapiens
File Sets Using This Measurement Set as an Input
1 item
Accession
Type
Summary
Aliases
Lab
IGVFDS4011VZXE
AnalysisSet
activation Perturb-seq: filtered feature barcode matrix
charles-gersbach:cd8-tcells-intermediate-analysis-set-perturb-seq-crispra
Charles Gersbach, Duke
Auxiliary Datasets
1 item
Accession
Type
Summary
Files
Aliases
Lab
IGVFDS9108NLTS
AuxiliarySet
gRNA sequencing for CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
IGVFFI9294QFQU
,
IGVFFI5265TJLC
,
IGVFFI3307YHIY
,
IGVFFI8799MGVV
,
IGVFFI2004LGCX
,
IGVFFI9768TMWP
,
IGVFFI3564HSSU
,
IGVFFI9845KVUX
charles-gersbach:cd8-tcells-perturb-seq-crispra-grna-rep1
Charles Gersbach, Duke
Attribution
Award
HG012053
Principal Investigator(s)
Charles Gersbach
,
Gregory Crawford
,
Tim Reddy
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke