Home
Home
Search (⌘K or ⌃K)
Data
Methodology
Data Model
About
Help
Sign In
INDEXED
Home
/
MeasurementSet
/
IGVFDS6764PIJI
IGVFDS6764PIJI
released
Json view
File Set Type
experimental data
Summary
activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
Assay Term
in vitro CRISPR screen using single-cell RNA-seq
Sample Summaries
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
Construct Library Sets
IGVFDS0821YMMV
Guide (sgRNA) library targeting transcription start sites in 24 genes
Protocols
https://www.protocols.io/view/perturb-seq-characterizing-regulators-of-t-cell-fu-c5s9y6h6
https://www.protocols.io/view/crispra-tiling-screens-c5rfy53n
Aliases
charles-gersbach:cd8-tcells-perturb-seq-crispra-gex-rep1
Publications
Transcriptional and epigenetic regulators of human CD8+ T cell function identified through orthogonal CRISPR screens
Sequencing Results (Illumina)
8 items
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Associated seqspec Files
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI1893OEIP
fastq
reads
R1
1
IGVFFI4202MHFC
Illumina NovaSeq 6000
2
8.3 GB
Charles Gersbach, Duke
validated
IGVFFI2514FKSS
fastq
reads
R1
1
IGVFFI3921CPXY
Illumina NovaSeq 6000
4
8.4 GB
Charles Gersbach, Duke
validated
IGVFFI3522PYTD
fastq
reads
R2
1
IGVFFI4202MHFC
Illumina NovaSeq 6000
2
21 GB
Charles Gersbach, Duke
validated
IGVFFI5126PDVA
fastq
reads
R2
1
IGVFFI3921CPXY
Illumina NovaSeq 6000
4
21 GB
Charles Gersbach, Duke
validated
IGVFFI6949VPVV
fastq
reads
R2
1
IGVFFI2121DSKN
Illumina NovaSeq 6000
1
21 GB
Charles Gersbach, Duke
validated
IGVFFI7715PXEN
fastq
reads
R1
1
IGVFFI4327JRXJ
Illumina NovaSeq 6000
3
8.4 GB
Charles Gersbach, Duke
validated
IGVFFI7791HIRG
fastq
reads
R1
1
IGVFFI2121DSKN
Illumina NovaSeq 6000
1
8.4 GB
Charles Gersbach, Duke
validated
IGVFFI9683XENS
fastq
reads
R2
1
IGVFFI4327JRXJ
Illumina NovaSeq 6000
3
21 GB
Charles Gersbach, Duke
validated
Samples
Donors
File Sets Using This Measurement Set as an Input
Auxiliary Datasets
Attribution
Award
HG012053
Principal Investigator(s)
Charles Gersbach
,
Gregory Crawford
,
Tim Reddy
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke