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INDEXED
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Measurement Set
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IGVFDS6710CDSE
IGVFDS6710CDSE
released
Json view
File Set Type
experimental data
Summary
cas mediated mutagenesis (Variant FlowFISH) integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Assay Term
cas mediated mutagenesis
Sample Summaries
Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with a guide library
Construct Library Sets
IGVFDS2385DHZO
Guide (sgRNA) library targeting sequence variants in a genomic locus
Protocols
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
Aliases
jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep1-FFrep2-BinD_AmpliconSequencing
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM6665OGEE
InVitroSystem
Jurkat
Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with a guide library
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO1080XFGV
igvf:donor_of_Jurkat
male
Homo sapiens
Sequencing Results (Illumina)
48 items
1
2
3
4
5
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Associated seqspec Files
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI0637AJLX
fastq
reads
I2
1
Illumina NextSeq 550
AAGGATGT:CAGT
2.0 MB
Jesse Engreitz, Stanford
validated
IGVFFI0765HJEP
fastq
reads
I1
1
Illumina NextSeq 550
TGCTGCTG:CAGT
1.8 MB
Jesse Engreitz, Stanford
validated
IGVFFI0814ISRC
fastq
reads
R1
1
Illumina NextSeq 550
TCTGCAAG:CAGT
6.8 MB
Jesse Engreitz, Stanford
validated
IGVFFI0836ZQNB
fastq
reads
R2
1
Illumina NextSeq 550
TGCTGCTG:CAGT
9.4 MB
Jesse Engreitz, Stanford
validated
IGVFFI0847GSDH
fastq
reads
R1
1
Illumina NextSeq 550
TGTAACTC:CAGT
6.8 MB
Jesse Engreitz, Stanford
validated
IGVFFI0848OKJK
fastq
reads
I2
1
Illumina NextSeq 550
TAATGAAC:CAGT
2.1 MB
Jesse Engreitz, Stanford
validated
IGVFFI0880ZYUS
fastq
reads
R2
1
Illumina NextSeq 550
TTGTCTAT:CAGT
9.6 MB
Jesse Engreitz, Stanford
validated
IGVFFI1286YIFX
fastq
reads
R1
1
Illumina NextSeq 550
TGCTGCTG:CAGT
6.8 MB
Jesse Engreitz, Stanford
validated
IGVFFI1287KXUV
fastq
reads
R1
1
Illumina NextSeq 550
TTGAGCCT:CAGT
6.9 MB
Jesse Engreitz, Stanford
validated
IGVFFI1345NBXM
fastq
reads
I1
1
Illumina NextSeq 550
TAATGAAC:CAGT
2.1 MB
Jesse Engreitz, Stanford
validated
Control File Sets
1 item
Accession
Summary
Aliases
Lab
IGVFDS4869FXYY
cas mediated mutagenesis (Variant FlowFISH)
jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-Jurkat-PE2_Exp2_NegControl_AmpliconSequencing
Jesse Engreitz, Stanford
Attribution
Award
HG011972
Principal Investigator(s)
Anshul Kundaje
,
Jesse Engreitz
,
Marlene Rabinovitch
,
Lars Steinmetz
,
Michael Bassik
,
Thomas Quertermous
,
Will Greenleaf
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford