IGVFDS6704KMTH

released

File Set Type

experimental data

Summary

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

FAM3C

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Construct Library Sets

IGVFDS1891GTLU guide (sgRNA) library targeting accessible genome regions in many genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_FAM3C_Auxin0hrs-FF1-BinB-PCR1-Rep1_S110

jesse-engreitz:measurement_CRUDO_FAM3C_Auxin0hrs-FF1-BinB-PCR2-Rep1_S116

jesse-engreitz:measurement_CRUDO_FAM3C_Auxin0hrs-FF1-BinB-PCR3-Rep1_S164

jesse-engreitz:measurement_CRUDO_FAM3C_Auxin0hrs-FF1-BinB-PCR4-Rep1_S170

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM0069TVXJ

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS6449NYRM

AuxiliarySet

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFFI5340ERRF, IGVFFI3781TWXY, IGVFFI7194FCZO, IGVFFI5756XBGF

jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin0hrs-FF1-BinB-PCR1-Rep1_S110
jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin0hrs-FF1-BinB-PCR2-Rep1_S116
jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin0hrs-FF1-BinB-PCR3-Rep1_S164
jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin0hrs-FF1-BinB-PCR4-Rep1_S170

Jesse Engreitz, Stanford

IGVFDS7833JNUC

AuxiliarySet

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

IGVFFI2396MZVU