{
"@context": "/terms/",
"@id": "/measurement-sets/IGVFDS6517MOFU/",
"@type": [
"MeasurementSet",
"FileSet",
"Item"
],
"accession": "IGVFDS6517MOFU",
"aliases": [
"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR1_Rep1_S112",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR1_Rep2_S167",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR2_Rep1_S118",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR2_Rep2_S173",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR3_Rep1_S124",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR3_Rep2_S179",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR4_Rep1_S130",
"jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR4_Rep2_S185"
],
"assay_term": {
"@id": "/assay-terms/OBI_0003661/",
"assay_slims": [
"CRISPR screens"
],
"status": "released",
"term_name": "in vitro CRISPR screen using flow cytometry"
},
"audit": {
"INTERNAL_ACTION": [
{
"category": "missing publication",
"detail": "Measurement set [IGVFDS6517MOFU](/measurement-sets/IGVFDS6517MOFU/) has no `publications`. Released and archived file sets are expected to be associated with a publication.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_file_set_missing_publication",
"path": "/measurement-sets/IGVFDS6517MOFU/"
},
{
"category": "missing sequencing kit",
"detail": "Measurement set [IGVFDS6517MOFU](/measurement-sets/IGVFDS6517MOFU/) has sequence file(s) [IGVFFI7993WRUA](/sequence-files/IGVFFI7993WRUA/), [IGVFFI8689TPHH](/sequence-files/IGVFFI8689TPHH/), [IGVFFI1193HDXG](/sequence-files/IGVFFI1193HDXG/), [IGVFFI6277LDPG](/sequence-files/IGVFFI6277LDPG/), [IGVFFI1593YXMS](/sequence-files/IGVFFI1593YXMS/), [IGVFFI8232UPNC](/sequence-files/IGVFFI8232UPNC/), [IGVFFI3058GAYK](/sequence-files/IGVFFI3058GAYK/), [IGVFFI4264KJXK](/sequence-files/IGVFFI4264KJXK/) which lack specification of a `sequencing_kit`. Sequence files in a file set associated wtih bulk data should specify a sequencing kit.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_inconsistent_sequencing_kit",
"path": "/measurement-sets/IGVFDS6517MOFU/"
},
{
"category": "missing publication",
"detail": "Auxiliary set [IGVFDS9055BZJI](/auxiliary-sets/IGVFDS9055BZJI/) has no `publications`. Released and archived file sets are expected to be associated with a publication.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_file_set_missing_publication",
"path": "/auxiliary-sets/IGVFDS9055BZJI/"
},
{
"category": "missing publication",
"detail": "In vitro system [IGVFSM0361IFQU](/in-vitro-systems/IGVFSM0361IFQU/) has no `publications`. Released and archived samples are expected to be associated with a publication.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_sample_missing_publication",
"path": "/in-vitro-systems/IGVFSM0361IFQU/"
}
],
"WARNING": [
{
"category": "missing nucleic acid delivery",
"detail": "In vitro system [IGVFSM0361IFQU](/in-vitro-systems/IGVFSM0361IFQU/) has `construct_library_sets` but is missing `nucleic_acid_delivery`. Samples linked to construct library sets are expected to specify nucleic acid delivery method.",
"level": 40,
"level_name": "WARNING",
"name": "audit_missing_nucleic_acid_delivery",
"path": "/in-vitro-systems/IGVFSM0361IFQU/"
}
]
},
"auxiliary_sets": [
{
"@id": "/auxiliary-sets/IGVFDS9055BZJI/",
"accession": "IGVFDS9055BZJI",
"aliases": [
"jesse-engreitz:auxiliary_sortparams_CRUDO_MYC-Auxin6hrs-FF1"
],
"file_set_type": "cell sorting",
"status": "released"
}
],
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
"title": "Jesse Engreitz"
},
"title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
},
"construct_library_sets": [
{
"@id": "/construct-library-sets/IGVFDS7857SYCK/",
"accession": "IGVFDS7857SYCK",
"file_set_type": "guide library",
"integrated_content_files": [
{
"@id": "/tabular-files/IGVFFI3782KCTX/",
"accession": "IGVFFI3782KCTX",
"content_type": "guide RNA sequences",
"status": "released",
"summary": "guide RNA sequences"
}
],
"status": "released",
"summary": "guide (sgRNA) library targeting accessible genome regions in many genomic loci"
}
],
"control_for": [],
"creation_timestamp": "2024-05-21T17:34:56.935973+00:00",
"documents": [
"/documents/74d53b9c-ab1d-42e3-b679-d3a00ae3d842/"
],
"donors": [
{
"@id": "/human-donors/IGVFDO0384OZRW/",
"accession": "IGVFDO0384OZRW",
"aliases": [
"igvf:donor_of_HCT116"
],
"sex": "male",
"status": "released",
"taxa": "Homo sapiens"
}
],
"externally_hosted": false,
"file_set_type": "experimental data",
"files": [
{
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"accession": "IGVFFI7482TIZK",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR4_Rep1_S130"
],
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:50:52.183375+00:00",
"file_format": "yaml",
"file_size": 911,
"href": "/configuration-files/IGVFFI7482TIZK/@@download/IGVFFI7482TIZK.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/a0e7114f-79cf-48e3-8d5a-363f130d514a/IGVFFI7482TIZK.yaml.gz",
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.MYC-Auxin6hrs-FF1-BinB-PCR4_Rep1_S130.yaml",
"upload_status": "validation exempted"
},
{
"@id": "/configuration-files/IGVFFI8825EPCR/",
"accession": "IGVFFI8825EPCR",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR2_Rep1_S118"
],
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:49:53.020446+00:00",
"file_format": "yaml",
"file_size": 913,
"href": "/configuration-files/IGVFFI8825EPCR/@@download/IGVFFI8825EPCR.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/e714acf7-a2ba-4805-b619-48fd45dff607/IGVFFI8825EPCR.yaml.gz",
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.MYC-Auxin6hrs-FF1-BinB-PCR2_Rep1_S118.yaml",
"upload_status": "validation exempted"
},
{
"@id": "/sequence-files/IGVFFI7993WRUA/",
"accession": "IGVFFI7993WRUA",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR1_Rep2_S167"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-06-01T02:03:58.458025+00:00",
"file_format": "fastq",
"file_size": 21538815,
"href": "/sequence-files/IGVFFI7993WRUA/@@download/IGVFFI7993WRUA.fastq.gz",
"s3_uri": "s3://igvf-files/2024/06/01/4339bcca-60ac-4719-aabe-0d7a5fe625d3/IGVFFI7993WRUA.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008565/",
"status": "released",
"term_name": "Illumina HiSeq 2500"
},
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin6hrs-FF1-BinB-PCR1-Rep2_S167_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/configuration-files/IGVFFI4823SDRE/",
"accession": "IGVFFI4823SDRE",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR1_Rep1_S112"
],
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:49:23.590667+00:00",
"file_format": "yaml",
"file_size": 913,
"href": "/configuration-files/IGVFFI4823SDRE/@@download/IGVFFI4823SDRE.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/2d4ae808-d73e-4b46-a167-014645b2c367/IGVFFI4823SDRE.yaml.gz",
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.MYC-Auxin6hrs-FF1-BinB-PCR1_Rep1_S112.yaml",
"upload_status": "validation exempted"
},
{
"@id": "/configuration-files/IGVFFI1475ALYJ/",
"accession": "IGVFFI1475ALYJ",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR3_Rep1_S124"
],
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:50:22.664598+00:00",
"file_format": "yaml",
"file_size": 911,
"href": "/configuration-files/IGVFFI1475ALYJ/@@download/IGVFFI1475ALYJ.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/84c02af7-d303-4bae-8fb1-65868b34d378/IGVFFI1475ALYJ.yaml.gz",
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.MYC-Auxin6hrs-FF1-BinB-PCR3_Rep1_S124.yaml",
"upload_status": "validation exempted"
},
{
"@id": "/configuration-files/IGVFFI1900MCOG/",
"accession": "IGVFFI1900MCOG",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR3_Rep2_S179"
],
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:50:37.651034+00:00",
"file_format": "yaml",
"file_size": 912,
"href": "/configuration-files/IGVFFI1900MCOG/@@download/IGVFFI1900MCOG.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/606dbf87-6f89-4e31-89b3-a07ac71f9946/IGVFFI1900MCOG.yaml.gz",
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.MYC-Auxin6hrs-FF1-BinB-PCR3_Rep2_S179.yaml",
"upload_status": "validation exempted"
},
{
"@id": "/configuration-files/IGVFFI5156TDWH/",
"accession": "IGVFFI5156TDWH",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR2_Rep2_S173"
],
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:50:08.092968+00:00",
"file_format": "yaml",
"file_size": 911,
"href": "/configuration-files/IGVFFI5156TDWH/@@download/IGVFFI5156TDWH.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/e0b22325-638a-4be8-8757-964099f0a983/IGVFFI5156TDWH.yaml.gz",
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.MYC-Auxin6hrs-FF1-BinB-PCR2_Rep2_S173.yaml",
"upload_status": "validation exempted"
},
{
"@id": "/sequence-files/IGVFFI8689TPHH/",
"accession": "IGVFFI8689TPHH",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR2_Rep2_S173"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-06-01T02:04:38.923088+00:00",
"file_format": "fastq",
"file_size": 20088225,
"href": "/sequence-files/IGVFFI8689TPHH/@@download/IGVFFI8689TPHH.fastq.gz",
"s3_uri": "s3://igvf-files/2024/06/01/aae540ce-7caf-4b9a-898e-fecc5e12e527/IGVFFI8689TPHH.fastq.gz",
"sequencing_platform": {
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"status": "released",
"term_name": "Illumina HiSeq 2500"
},
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin6hrs-FF1-BinB-PCR2-Rep2_S173_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/configuration-files/IGVFFI9689CPCW/",
"accession": "IGVFFI9689CPCW",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR4_Rep2_S185"
],
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T20:51:08.781958+00:00",
"file_format": "yaml",
"file_size": 912,
"href": "/configuration-files/IGVFFI9689CPCW/@@download/IGVFFI9689CPCW.yaml.gz",
"s3_uri": "s3://igvf-files/2024/06/11/e4e6671a-9269-42d8-8e46-ba32e2c7d1a1/IGVFFI9689CPCW.yaml.gz",
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"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.MYC-Auxin6hrs-FF1-BinB-PCR4_Rep2_S185.yaml",
"upload_status": "validation exempted"
},
{
"@id": "/sequence-files/IGVFFI1193HDXG/",
"accession": "IGVFFI1193HDXG",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR4_Rep2_S185"
],
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"controlled_access": false,
"creation_timestamp": "2024-06-01T02:05:56.450179+00:00",
"file_format": "fastq",
"file_size": 15438986,
"href": "/sequence-files/IGVFFI1193HDXG/@@download/IGVFFI1193HDXG.fastq.gz",
"s3_uri": "s3://igvf-files/2024/06/01/4a388e7d-5326-4275-ac2f-a82847edf780/IGVFFI1193HDXG.fastq.gz",
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},
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},
{
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],
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"creation_timestamp": "2024-06-11T20:49:38.515876+00:00",
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},
{
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"href": "/sequence-files/IGVFFI6277LDPG/@@download/IGVFFI6277LDPG.fastq.gz",
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},
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},
{
"@id": "/sequence-files/IGVFFI1593YXMS/",
"accession": "IGVFFI1593YXMS",
"aliases": [
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],
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},
{
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],
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"href": "/sequence-files/IGVFFI8232UPNC/@@download/IGVFFI8232UPNC.fastq.gz",
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},
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"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI3058GAYK/",
"accession": "IGVFFI3058GAYK",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR3_Rep2_S179"
],
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"href": "/sequence-files/IGVFFI3058GAYK/@@download/IGVFFI3058GAYK.fastq.gz",
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},
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"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI4264KJXK/",
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"aliases": [
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},
"status": "released",
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin6hrs-FF1-BinB-PCR1-Rep1_S112_R1_001.fastq.gz",
"upload_status": "validated"
}
],
"input_for": [
"/analysis-sets/IGVFDS4544YEQM/"
],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"notes": "The readout 2e41e624-f6c7-4f0c-a07e-bc36cefdca39 was removed from this measurement set.",
"preferred_assay_title": "CRISPR FlowFISH screen",
"protocols": [
"https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1"
],
"release_timestamp": "2024-07-10T19:47:46.139324+00:00",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM0361IFQU/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM0361IFQU",
"aliases": [
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR1_Rep1_S112",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR1_Rep2_S167",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR2_Rep1_S118",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR2_Rep2_S173",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR3_Rep1_S124",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR3_Rep2_S179",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR4_Rep1_S130",
"jesse-engreitz:in-vitro-system_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR4_Rep2_S185"
],
"classifications": [
"cell line"
],
"institutional_certificates": [],
"modifications": [
{
"@id": "/degron-modifications/95081d1b-08b4-49ed-b324-e53fc43d8456/",
"modality": "degradation",
"status": "released"
},
{
"@id": "/crispr-modifications/8543fa06-1720-4e7d-8e8f-7e24c1b1cf79/",
"modality": "interference",
"status": "released"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002824/",
"@type": [
"SampleTerm",
"OntologyTerm",
"Item"
],
"aliases": [
"igvf:HCT116"
],
"status": "released",
"summary": "HCT116",
"term_name": "HCT116"
}
],
"status": "released",
"summary": "Homo sapiens (male) HCT116 cell line (sorting details: FACS bin B for 10-20% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library",
"taxa": "Homo sapiens",
"treatments": [
{
"@id": "/treatments/d35868d1-c45b-49ae-8ff3-a9c0c22551c2/",
"purpose": "activation",
"status": "released",
"summary": "Treatment of 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours",
"treatment_type": "chemical"
}
]
}
],
"schema_version": "26",
"status": "released",
"submitted_by": {
"@id": "/users/3bd6f74c-8cf3-4c35-a94d-0c7c11654815/",
"title": "Chongyon Park"
},
"submitted_files_timestamp": "2024-06-01T02:03:38.334076+00:00",
"summary": "mixed CRISPR FlowFISH screen sorted on the expression of MYC integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci",
"targeted_genes": [
{
"@id": "/genes/ENSG00000136997/",
"geneid": "ENSG00000136997",
"name": "MYC proto-oncogene, bHLH transcription factor",
"status": "released",
"symbol": "MYC",
"synonyms": [
"bHLHe39",
"myc-related translation/localization regulatory factor",
"MYC proto-oncogene, bHLH transcription factor",
"MYC",
"c-Myc",
"v-myc avian myelocytomatosis viral oncogene homolog",
"proto-oncogene c-Myc",
"transcription factor p64",
"myc proto-oncogene protein",
"avian myelocytomatosis viral oncogene homolog",
"MRTL",
"class E basic helix-loop-helix protein 39",
"MYCC",
"v-myc myelocytomatosis viral oncogene homolog"
]
}
],
"uuid": "234860e2-bf47-44a6-8ae4-b59a796a9dcf"
}