IGVFDS6475UHMP

released

File Set Type

experimental data

Summary

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

MYC

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Construct Library Sets

IGVFDS7857SYCK guide (sgRNA) library targeting accessible genome regions in many genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR1_Rep1_S116

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR1_Rep2_S171

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR2_Rep1_S122

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR2_Rep2_S177

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR3_Rep1_S128

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR3_Rep2_S183

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR4_Rep1_S134

jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR4_Rep2_S189

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM3998EEZS

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS2372JFBI

AuxiliarySet

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFFI7923UEBL, IGVFFI7683TXPK, IGVFFI5849CCCH, IGVFFI3283FABB, IGVFFI1366IKMD, IGVFFI1641ICFG, IGVFFI9101IEYD, IGVFFI3695WJWY

jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR1_Rep1_S116
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR1_Rep2_S171
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR2_Rep1_S122
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR2_Rep2_S177
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR3_Rep1_S128
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR3_Rep2_S183
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR4_Rep1_S134
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinF-PCR4_Rep2_S189

Jesse Engreitz, Stanford

IGVFDS9055BZJI

AuxiliarySet

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

IGVFFI6885TVYB