{
"@context": "/terms/",
"@id": "/measurement-sets/IGVFDS6464FKSV/",
"@type": [
"MeasurementSet",
"FileSet",
"Item"
],
"accession": "IGVFDS6464FKSV",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep1-FFrep1-BinD_AmpliconSequencing"
],
"assay_term": {
"@id": "/assay-terms/OBI_0003661/",
"status": "released",
"term_name": "in vitro CRISPR screen using flow cytometry"
},
"audit": {
"INTERNAL_ACTION": [
{
"category": "mismatched status",
"detail": "Released in vitro system [IGVFSM3005IBKC](/in-vitro-systems/IGVFSM3005IBKC/) has in progress subobject construct library set [IGVFDS6377KNCH](/construct-library-sets/IGVFDS6377KNCH/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/in-vitro-systems/IGVFSM3005IBKC/"
},
{
"category": "missing sequencing kit",
"detail": "Measurement set [IGVFDS6464FKSV](/measurement-sets/IGVFDS6464FKSV/) has sequence file(s) [IGVFFI7038TVZR](/sequence-files/IGVFFI7038TVZR/), [IGVFFI7067YEEY](/sequence-files/IGVFFI7067YEEY/), [IGVFFI1220EQNX](/sequence-files/IGVFFI1220EQNX/), [IGVFFI4319QLFW](/sequence-files/IGVFFI4319QLFW/), [IGVFFI4422FYMH](/sequence-files/IGVFFI4422FYMH/), [IGVFFI5343CBUO](/sequence-files/IGVFFI5343CBUO/), [IGVFFI8119DTEV](/sequence-files/IGVFFI8119DTEV/), [IGVFFI9565FPSM](/sequence-files/IGVFFI9565FPSM/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_inconsistent_sequencing_kit",
"path": "/measurement-sets/IGVFDS6464FKSV/"
},
{
"category": "missing sequence specification",
"detail": "Measurement set [IGVFDS6464FKSV](/measurement-sets/IGVFDS6464FKSV/) has sequence file(s): [IGVFFI7038TVZR](/sequence-files/IGVFFI7038TVZR/), [IGVFFI7067YEEY](/sequence-files/IGVFFI7067YEEY/), [IGVFFI1220EQNX](/sequence-files/IGVFFI1220EQNX/), [IGVFFI4319QLFW](/sequence-files/IGVFFI4319QLFW/), [IGVFFI4422FYMH](/sequence-files/IGVFFI4422FYMH/), [IGVFFI5343CBUO](/sequence-files/IGVFFI5343CBUO/), [IGVFFI8119DTEV](/sequence-files/IGVFFI8119DTEV/), [IGVFFI9565FPSM](/sequence-files/IGVFFI9565FPSM/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_missing_seqspec",
"path": "/measurement-sets/IGVFDS6464FKSV/"
}
],
"WARNING": [
{
"category": "missing auxiliary set",
"detail": "Measurement set [IGVFDS6464FKSV](/measurement-sets/IGVFDS6464FKSV/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
"level": 40,
"level_name": "WARNING",
"name": "audit_missing_auxiliary_set",
"path": "/measurement-sets/IGVFDS6464FKSV/"
}
]
},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
"title": "Jesse Engreitz"
},
"title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
},
"control_file_sets": [
{
"@id": "/measurement-sets/IGVFDS9477ARWI/",
"accession": "IGVFDS9477ARWI",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-Jurkat-PE2_Exp1_NegControl_AmpliconSequencing",
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-Jurkat-PE2_Exp1_NegControl_AmpliconSequencing"
],
"status": "released"
}
],
"control_for": [],
"creation_timestamp": "2023-11-30T22:47:49.259585+00:00",
"donors": [
{
"@id": "/human-donors/IGVFDO1080XFGV/",
"accession": "IGVFDO1080XFGV",
"aliases": [
"igvf:donor_of_Jurkat"
],
"sex": "male",
"status": "released",
"taxa": "Homo sapiens"
}
],
"externally_hosted": false,
"file_set_type": "experimental data",
"files": [
{
"@id": "/sequence-files/IGVFFI7038TVZR/",
"accession": "IGVFFI7038TVZR",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep1-FFrep1-BinD-PCRrep2_R1"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T20:04:24.565011+00:00",
"file_format": "fastq",
"file_size": 7395999,
"href": "/sequence-files/IGVFFI7038TVZR/@@download/IGVFFI7038TVZR.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/eff4e856-eec8-4191-9123-83c52d236419/IGVFFI7038TVZR.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S12-BioRep1-FFRep1-BinD-PCRrep2_S173_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI7067YEEY/",
"accession": "IGVFFI7067YEEY",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep1-FFrep1-BinD-PCRrep1_R2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T19:39:31.737889+00:00",
"file_format": "fastq",
"file_size": 13444080,
"href": "/sequence-files/IGVFFI7067YEEY/@@download/IGVFFI7067YEEY.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/3aa0eedc-e74b-4188-9ec1-6045ca930d8b/IGVFFI7067YEEY.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S12-BioRep1-FFRep1-BinD-PCRrep1_S116_R2_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI1220EQNX/",
"accession": "IGVFFI1220EQNX",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep1-FFrep1-BinD-PCRrep2_R2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T20:12:42.990148+00:00",
"file_format": "fastq",
"file_size": 13704580,
"href": "/sequence-files/IGVFFI1220EQNX/@@download/IGVFFI1220EQNX.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/a06d9457-7133-4f20-afac-32fc56d0b8e7/IGVFFI1220EQNX.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S12-BioRep1-FFRep1-BinD-PCRrep2_S173_R2_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI4319QLFW/",
"accession": "IGVFFI4319QLFW",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep1-FFrep1-BinD-PCRrep1_I1"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T19:48:04.664251+00:00",
"file_format": "fastq",
"file_size": 3063609,
"href": "/sequence-files/IGVFFI4319QLFW/@@download/IGVFFI4319QLFW.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/8d815d1a-64b2-4ef2-8858-7eb2da7c700e/IGVFFI4319QLFW.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S12-BioRep1-FFRep1-BinD-PCRrep1_S116_I1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI4422FYMH/",
"accession": "IGVFFI4422FYMH",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep1-FFrep1-BinD-PCRrep1_R1"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T19:31:10.830006+00:00",
"file_format": "fastq",
"file_size": 7273669,
"href": "/sequence-files/IGVFFI4422FYMH/@@download/IGVFFI4422FYMH.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/0b85bc21-100e-41d2-a2be-8e0ca53003c4/IGVFFI4422FYMH.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S12-BioRep1-FFRep1-BinD-PCRrep1_S116_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI5343CBUO/",
"accession": "IGVFFI5343CBUO",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep1-FFrep1-BinD-PCRrep2_I2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T20:29:30.689566+00:00",
"file_format": "fastq",
"file_size": 3098098,
"href": "/sequence-files/IGVFFI5343CBUO/@@download/IGVFFI5343CBUO.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/4c1e8b8b-6523-4889-b7a4-97889a8a6f27/IGVFFI5343CBUO.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S12-BioRep1-FFRep1-BinD-PCRrep2_S173_I2_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI8119DTEV/",
"accession": "IGVFFI8119DTEV",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep1-FFrep1-BinD-PCRrep2_I1"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T20:21:14.073842+00:00",
"file_format": "fastq",
"file_size": 3243819,
"href": "/sequence-files/IGVFFI8119DTEV/@@download/IGVFFI8119DTEV.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/b3927e15-1b67-4e1b-93f9-9cb5a9011a7c/IGVFFI8119DTEV.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S12-BioRep1-FFRep1-BinD-PCRrep2_S173_I1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI9565FPSM/",
"accession": "IGVFFI9565FPSM",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep1-FFrep1-BinD-PCRrep1_I2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T19:56:16.973687+00:00",
"file_format": "fastq",
"file_size": 3035198,
"href": "/sequence-files/IGVFFI9565FPSM/@@download/IGVFFI9565FPSM.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/71859fbd-87d8-4eab-b12f-0a869befa1e1/IGVFFI9565FPSM.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S12-BioRep1-FFRep1-BinD-PCRrep1_S116_I2_001.fastq.gz",
"upload_status": "validated"
}
],
"input_for": [
"/analysis-sets/IGVFDS1863TJOE/"
],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"notes": "The readout 2e41e624-f6c7-4f0c-a07e-bc36cefdca39 was removed from this measurement set. This measurement set previously used Variant FlowFISH as a preferred_assay_title, but this enum is now removed. So it has been defaulted to Variant-EFFECTS.",
"preferred_assay_title": "Variant-EFFECTS",
"protocols": [
"https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
],
"release_timestamp": "2023-12-20T23:10:57.271829+00:00",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM3005IBKC/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM3005IBKC",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep1-FFrep1-BinD"
],
"classifications": [
"cell line"
],
"construct_library_sets": [
{
"@id": "/construct-library-sets/IGVFDS6377KNCH/",
"accession": "IGVFDS6377KNCH",
"file_set_type": "guide library",
"status": "in progress",
"summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
}
],
"modifications": [
{
"@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
"modality": "prime editing",
"status": "released"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"@type": [
"SampleTerm",
"OntologyTerm",
"Item"
],
"aliases": [
"igvf:Jurkat"
],
"status": "released",
"summary": "Jurkat",
"term_name": "Jurkat"
}
],
"status": "released",
"summary": "Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 55-65% expression of PPIF) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
"taxa": "Homo sapiens",
"treatments": [
{
"@id": "/treatments/df02c0d4-f796-4f77-bfbc-388cdbfcc615/",
"status": "released",
"treatment_term_name": "T-cell surface glycoprotein CD3 gamma chain"
},
{
"@id": "/treatments/42b3c61d-38de-4bd2-bb39-33c125a417cb/",
"status": "released",
"treatment_term_name": "phorbol 13-acetate 12-myristate"
}
]
}
],
"schema_version": "23",
"status": "released",
"submitted_by": {
"@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
"title": "Michael Montgomery"
},
"submitted_files_timestamp": "2023-12-05T19:31:10.830006+00:00",
"summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
"targeted_genes": [
{
"@id": "/genes/ENSG00000108179/",
"geneid": "ENSG00000108179",
"name": "peptidylprolyl isomerase F",
"status": "released",
"symbol": "PPIF",
"synonyms": [
"rotamase F",
"CYP3",
"cyclophilin F",
"mitochondrial cyclophilin",
"peptidyl-prolyl cis-trans isomerase, mitochondrial",
"CypD",
"Cyp-D",
"cyclophilin 3",
"cyclophilin D",
"CyP-M",
"peptidyl-prolyl cis-trans isomerase F, mitochondrial",
"PPIase F"
]
}
],
"uuid": "cf27489d-35e4-4623-b244-154494996039"
}