IGVFDS6407EOOV

released

File Set Type

experimental data

Summary

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

KITLG

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Construct Library Sets

IGVFDS7846UISG guide (sgRNA) library targeting accessible genome regions in many genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinC-PCR1_S51

jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinC-PCR2_S57

jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinC-PCR3_S147

jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF1-BinC-PCR4_S153

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM0623GQRJ

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS1630KVRE

AuxiliarySet

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFFI7346SZCE, IGVFFI5577IRJO, IGVFFI0388ZLUI, IGVFFI9213RYPE

jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin6hrs-FF1-BinC-PCR1_S51
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin6hrs-FF1-BinC-PCR2_S57
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin6hrs-FF1-BinC-PCR3_S147
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin6hrs-FF1-BinC-PCR4_S153

Jesse Engreitz, Stanford

IGVFDS4521OAKZ

AuxiliarySet

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

IGVFFI3484IZUF