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IGVFDS6376TFTJ

released
File Set Type
experimental data
Summary
mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Assay Term
in vitro CRISPR screen using flow cytometry
Targeted Genes
Sample Summaries
  • HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
Protocols
Aliases
jesse-engreitz:measurement_CRUDO_CCND1_Auxin0hrs-FF1-BinA-PCR1-Rep1_S217
jesse-engreitz:measurement_CRUDO_CCND1_Auxin0hrs-FF1-BinA-PCR2-Rep1_S223
jesse-engreitz:measurement_CRUDO_CCND1_Auxin0hrs-FF1-BinA-PCR3-Rep1_S259
jesse-engreitz:measurement_CRUDO_CCND1_Auxin0hrs-FF1-BinA-PCR4-Rep1_S265

Sequencing Results

4 items
Read Names
fastq
reads
1
GACCAGGA:GCCA
25 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
AACGCATT:TTGA
22 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
AACGCATT:GCCA
30 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
GACCAGGA:TTGA
17 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Associated Construct Library Sets
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Associated Construct Library Sets

1 item
Accession
Summary
Library Design Files
guide (sgRNA) library targeting accessible genome regions in many genomic loci

Auxiliary Datasets

1 item
Accession
Type
Summary
Aliases
Lab
AuxiliarySet
gRNA sequencing for mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin0hrs-FF1-BinA-PCR1-Rep1_S217
  • jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin0hrs-FF1-BinA-PCR2-Rep1_S223
  • jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin0hrs-FF1-BinA-PCR3-Rep1_S259
  • jesse-engreitz:auxiliary_CRUDO_CCND1_Auxin0hrs-FF1-BinA-PCR4-Rep1_S265
Jesse Engreitz, Stanford

Documents

1 item
Page
Description
Type
Download
Preview
overview of experimental design
experimental protocol

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford