IGVFDS6323AUPJ

released

File Set Type

experimental data

Summary

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

MYC

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Construct Library Sets

IGVFDS7857SYCK guide (sgRNA) library targeting accessible genome regions in many genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR1_Rep1_S5

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR1_Rep2_S60

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR2_Rep1_S11

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR2_Rep2_S66

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR3_Rep1_S17

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR3_Rep2_S72

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR4_Rep1_S23

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR4_Rep2_S78

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM7084WNRY

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS5581ABLR

AuxiliarySet

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFFI9575IHNR, IGVFFI7367BUVJ, IGVFFI1014ZQSZ, IGVFFI8734SFUZ, IGVFFI4836TTVK, IGVFFI1666IWMV, IGVFFI0132JLWC, IGVFFI5386XIKZ

jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR1_Rep1_S5
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR1_Rep2_S60
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR2_Rep1_S11
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR2_Rep2_S66
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR3_Rep1_S17
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR3_Rep2_S72
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR4_Rep1_S23
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR4_Rep2_S78

Jesse Engreitz, Stanford

IGVFDS5951VSHM

AuxiliarySet

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

IGVFFI2212HXUF